Fast haptoglobin phenotyping based on microchip electrophoresis

Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer’s disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection

“…Additionally, other analytical methods for haptoglobin phenotyping by capillary electrophoresis were published [19][20][21][22]. As noted above, proteins are usually quantified by conventional immunoassays based on the reaction of antibodies and antigens.…”

Microfluidics technology: future prospects for molecular diagnostics

“…Huang et al 78 developed a method based on microchip electrophoresis coupled with laser-induced fluorescence detection to phenotype haptoglobin (Hp), a biomarker for the liver cancer. The microfluidic device was fabricated with glass.…”

Applications of microfluidics and microchip electrophoresis for potential clinical biomarker analysis

“…Different Hp phenotypes have recently been shown to have association with major diseases: the Hp 2-2 phenotype is correlated with higher risk of developing refractory hypertension and schizophrenia [55,56]; in contrast, Hp 2-2 appears to have protective benefit while Hp 1-1 increases the risk for cervical cancer. Huang et al [57] tested microchip electrophoresis for Hp phenotyping and showed that fluorescently labeled Hp from a liver cancer patient's serum had lower Hp 2-2 levels compared to normal serum. Hp was only clearly detected in serum upon depletion of the abundant human serum albumin and immunoglobulins.…”