Fast generation of forebrain oligodendrocyte spheroids from human embryonic stem cells by transcription factors

Ling, Ma; Mei, Yuting; Xu, Peibo; Cheng, Yan; You, Zhiwen; Ji, Xiaoli; Zhuang, Deyi; Zhou, Wenhao; Chen, Yuejun; Xiong, Man

doi:10.1016/j.isci.2022.105172

Cited by 5 publications

(6 citation statements)

References 37 publications

(50 reference statements)

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“…Neural differentiation was induced using a previously described method. [ 25 ] Briefly, when H9 colonies reached 80% confluence, they were treated with dispase for chemical digestion. The digested cells were then suspended in a T‐25 flask (Corning, NY, USA) containing hESCs medium for the formation of embryoid bodies.…”

Section: Methodsmentioning

confidence: 99%

Human Breast Milk Exosomal miRNAs are Influenced by Premature Delivery and Affect Neurodevelopment

Ma,

Huo,

Tang

et al. 2024

Molecular Nutrition Food Res

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ScopeThis study investigates the exosomal microRNA (miRNA) profiles of term and preterm breast milk, including the most abundant and differentially expressed (DE) miRNAs, and their impact on neurodevelopment in infants.Methods and ResultsMature milk is collected from the mothers of term and preterm infants. Using high‐throughput sequencing and subsequent data analysis, exosomal miRNA profiles of term and preterm human breast milk (HBM) are acquired and it is found that the let‐7 and miR‐148 families are the most abundant miRNAs. Additionally, 23 upregulated and 15 downregulated miRNAs are identified. MiR‐3168 is the most upregulated miRNA in preterm HBM exosome, exhibiting targeting activity toward multiple genes involved in the SMAD and MAPK signaling pathways and playing a crucial role in early neurodevelopment. Additionally, the effects of miR‐3168 on neurodevelopment is confirmed and it is determined that it is an essential factor in the differentiation of neural stem cells (NSCs).ConclusionThis study demonstrates that miRNA expression in breast milk exosomes can be influenced by preterm delivery, thereby potentially impacting neurodevelopment in preterm infants.

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Section: Methodsmentioning

confidence: 99%

Human Breast Milk Exosomal miRNAs are Influenced by Premature Delivery and Affect Neurodevelopment

Ma,

Huo,

Tang

et al. 2024

Molecular Nutrition Food Res

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“…An additional study [67] was carried out to minimize the timing required for OPCs in 2D and 3D cultures. To achieve this goal, they overexpressed two transcription factors SOX10 and OLIG2 through lentiviral transduction.…”

Section: Oligodendrocytes and Myelin In Ipsc-derived Organoidsmentioning

confidence: 99%

Identity and Maturity of iPSC-Derived Oligodendrocytes in 2D and Organoid Systems

Zeldich,

Rajkumar

2024

Cells

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Oligodendrocytes originating in the brain and spinal cord as well as in the ventral and dorsal domains of the neural tube are transcriptomically and functionally distinct. These distinctions are also reflected in the ultrastructure of the produced myelin, and the susceptibility to myelin-related disorders, which highlights the significance of the choice of patterning protocols in the differentiation of induced pluripotent stem cells (iPSCs) into oligodendrocytes. Thus, our first goal was to survey the different approaches applied to the generation of iPSC-derived oligodendrocytes in 2D culture and in organoids, as well as reflect on how these approaches pertain to the regional and spatial fate of the generated oligodendrocyte progenitors and myelinating oligodendrocytes. This knowledge is increasingly important to disease modeling and future therapeutic strategies. Our second goal was to recap the recent advances in the development of oligodendrocyte-enriched organoids, as we explore their relevance to a regional specification alongside their duration, complexity, and maturation stages of oligodendrocytes and myelin biology. Finally, we discuss the shortcomings of the existing protocols and potential future explorations.

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“…Verfaillie and colleagues described that overexpression of a single transcription factor, Sox10, was sufficient to generate similar levels of O4+ cells from human PSCs derived NSCs within 22 days [ 147 ]. In addition to the generation of spinal cord OPCs and oligodendrocytes via the use of caudal morphogen RA, several RA-independent approaches that favor telencephalic OPC generation have been reported [ 143 , 148 , 149 ]. A study accelerated the production of RA-independent telencephalic oligodendrocytes by enhancing neural induction using dual SMAD inhibition in conjunction with the tankase inhibitor XAV 939 (antagonizing WNT signaling) [ 149 ].…”

Section: The Directional Differentiation Of Nscs From Different Sourc...mentioning

confidence: 99%

“…A study accelerated the production of RA-independent telencephalic oligodendrocytes by enhancing neural induction using dual SMAD inhibition in conjunction with the tankase inhibitor XAV 939 (antagonizing WNT signaling) [ 149 ]. More recently, Xiong and colleagues first promoted the generation of ventral forebrain NSCs of ESCs by dual SMAD inhibition and activation SHH signals, and subsequently demonstrated that overexpression of Sox10 and Olig2 in these cells was sufficient to generate forebrain mature oligodendrocytes at day 40 of differentiation [ 148 ].…”

Section: The Directional Differentiation Of Nscs From Different Sourc...mentioning

confidence: 99%

Directional induction of neural stem cells, a new therapy for neurodegenerative diseases and ischemic stroke

et al. 2023

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Due to the limited capacity of the adult mammalian brain to self-repair and regenerate, neurological diseases, especially neurodegenerative disorders and stroke, characterized by irreversible cellular damage are often considered as refractory diseases. Neural stem cells (NSCs) play a unique role in the treatment of neurological diseases for their abilities to self-renew and form different neural lineage cells, such as neurons and glial cells. With the increasing understanding of neurodevelopment and advances in stem cell technology, NSCs can be obtained from different sources and directed to differentiate into a specific neural lineage cell phenotype purposefully, making it possible to replace specific cells lost in some neurological diseases, which provides new approaches to treat neurodegenerative diseases as well as stroke. In this review, we outline the advances in generating several neuronal lineage subtypes from different sources of NSCs. We further summarize the therapeutic effects and possible therapeutic mechanisms of these fated specific NSCs in neurological disease models, with special emphasis on Parkinson’s disease and ischemic stroke. Finally, from the perspective of clinical translation, we compare the strengths and weaknesses of different sources of NSCs and different methods of directed differentiation, and propose future research directions for directed differentiation of NSCs in regenerative medicine.

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Fast generation of forebrain oligodendrocyte spheroids from human embryonic stem cells by transcription factors

Cited by 5 publications

References 37 publications

Human Breast Milk Exosomal miRNAs are Influenced by Premature Delivery and Affect Neurodevelopment

Human Breast Milk Exosomal miRNAs are Influenced by Premature Delivery and Affect Neurodevelopment

Identity and Maturity of iPSC-Derived Oligodendrocytes in 2D and Organoid Systems

Directional induction of neural stem cells, a new therapy for neurodegenerative diseases and ischemic stroke

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