Fast Gel Permeation Chromatography Part II: A Study of Operational Parameters

Theory of concentration effects in gel permeation chromatography

“…[77] = KMa (17) which under the theta conditions is reduced in Equation 15. In preceding equations, M0 is half the molecular weight of the repeating monomer unit in a vinyl polymer, ß (in Á) is the effective bond length (10). It also holds that gx = / 0 (18) and…”

“…Using the latter observations, it was suggested that the thermodynamic quality of the solvent should be estimated from concentration effects (14). It was also observed that the mutual arrangement of the individual columns affected the concentration dependence of the elution volume (15), and that, with increasing flow of the solvent, the concentration dependence of the elution volume decreased (16,17). An increase in the width of the elution curve with increasing concentration and volume of the injected polymer solution was observed by several authors (18-20).…”