Fast ferritin immunoassay on PDMS microchips

Schrott, Walter; Nebyla, Marek; Meisterová, Lucie; Přibyl, Michal

doi:10.2478/s11696-010-0079-6

Cited by 8 publications

(7 citation statements)

References 48 publications

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“…For signal processing to quantify the analyte, the device uses a laboratory size fluorescence microscope. Another ferritin immunoassay device that uses microfluidic approach was proposed by Schrott et al [ 32 , 33 ] who demonstrated eight-channel poly(dimethylsiloxane) (PDMS)–based ferritin immunoassay [ Figure 1 D]. The ferritin immunoassay was performed using proteins such as rIgG [Rockland 200-401-090, anti-ferritin (human spleen) rabbit IgG], antigen ferritin, secondary anti-body b-rIgG [Rockland 200-406-090, biotin-conjugated anti-ferritin (human spleen) rabbit IgG], and FITC-avidin.…”

Section: State-of-the-art Microfluidic Devices For Iron Deficiencymentioning

confidence: 99%

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Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia

Yap

Soair

Talik

et al. 2018

Sensors

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Over the past 20 years, rapid technological advancement in the field of microfluidics has produced a wide array of microfluidic point-of-care (POC) diagnostic devices for the healthcare industry. However, potential microfluidic applications in the field of nutrition, specifically to diagnose iron deficiency anemia (IDA) detection, remain scarce. Iron deficiency anemia is the most common form of anemia, which affects billions of people globally, especially the elderly, women, and children. This review comprehensively analyzes the current diagnosis technologies that address anemia-related IDA-POC microfluidic devices in the future. This review briefly highlights various microfluidics devices that have the potential to detect IDA and discusses some commercially available devices for blood plasma separation mechanisms. Reagent deposition and integration into microfluidic devices are also explored. Finally, we discuss the challenges of insights into potential portable microfluidic systems, especially for remote IDA detection.

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Section: State-of-the-art Microfluidic Devices For Iron Deficiencymentioning

confidence: 99%

“…( ii ) Architectural diagram of the test column [ 30 ]. ( D ) Eight-channel PDMS microfluidic device for ferritin immunoassay detection demonstrated by Schrott et al [ 32 ].…”

Section: Figurementioning

confidence: 99%

Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia

Yap

Soair

Talik

et al. 2018

Sensors

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“…Steigert et al developed a LOD platform based on colorimetric assay that can directly measure the concentration of hemoglobin from only 2 mL of whole blood within 100 s. 85 Microfluidic detections of serum ferritin have also been reported by several groups using on-chip immunoassays. [86][87][88] Through the integration of pneumatic microvalves, Kartalov et al realized a high-throughput, multi-antigen microfluidic fluorescence immunoassay system, which can simultaneously measure serum ferritin, CRP and several other serum proteins. 88 Recently, the first monolithically fabricated microfluidic electrochemical sensor capable of measuring zinc in serum was reported.…”

Section: Micronutrientsmentioning

confidence: 99%

Microfluidic opportunities in the field of nutrition

Kiehne

Sinoway

et al. 2013

Lab Chip

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Nutrition has always been closely related to human health, which is a constant motivational force driving research in a variety of disciplines. Over the years, the rapidly emerging field of microfluidics has been pushing forward the healthcare industry with the development of microfluidic-based, point-of-care (POC) diagnostic devices. Though a great deal of work has been done in developing microfluidic platforms for disease diagnoses, potential microfluidic applications in the field of nutrition remain largely unexplored. In this Focus article, we would like to investigate the potential chances for microfluidics in the field of nutrition. We will first highlight some of the recent advances in microfluidic blood analysis systems that have the capacity to detect biomarkers of nutrition. Then we will examine existing examples of microfluidic devices for the detection of specific biomarkers of nutrition or nutrient content in food. Finally, we will discuss the challenges in this field and provide some insight into the future of applied microfluidics in nutrition.

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“…wherein fluorescein isothiocyanate was used as the fluorescent probe for the detection purpose. The method was able to detect ferritin at a very high concentration (10 000 ng·mL –1 ) but was not able to sense ferritin at a low concentration range (less than 100 ng·mL –1 ), which is again a drawback . The most recent work on ferritin estimation shows the use of a conjugate of gold and carbon dots for the color as well as a fluorescence-based dual probe for ferritin.…”

Section: Introductionmentioning

confidence: 99%

“…The method was able to detect ferritin at a very high concentration (10 000 ng•mL −1 ) but was not able to sense ferritin at a low concentration range (less than 100 ng•mL −1 ), which is again a drawback. 31 The most recent work on ferritin estimation shows the use of a conjugate of gold and carbon dots for the color as well as a fluorescencebased dual probe for ferritin. The method had a linear range of 1−120 ng•mL −1 which does not fully cover the clinical range making it a demerit.…”

Section: ■ Introductionmentioning

confidence: 99%

Amine-Functionalized Graphene Quantum Dots for Fluorescence-Based Immunosensing of Ferritin

Garg

Vishwakarma

Sharma

et al. 2021

ACS Appl. Nano Mater.

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The current work demonstrates the fabrication and optimization of a fluorescence-based immunosensor for ferritin estimation wherein amine-functionalized graphene quantum dots (afGQDs) and methyl orange are used as a fluorophore–quencher couple. The synthesis of afGQDs is achieved by the hydrothermal method. The synthesized QDs were characterized using analytical techniques such as UV–vis, fluorescence, FTIR, and Raman spectroscopies, XRD diffraction studies, elemental analysis, and morphological studies through transmission electron microscopy. The QDs showed a quantum yield of 51%, which is one of the highest reported for this class of material. Exploiting the high fluorescence of this material, these afGQDs, were conjugated with antiferritin antibodies (Ab) for specific fluorescence-based immunosensing of ferritin. The conjugation was confirmed from contact angle measurements and electrophoresis, which confirmed successful bioconjugation. With methyl orange (MO) as the quencher, the Stern–Volmer plot showed a linear upward trend indicating a static quenching process. After elucidating the quenching mechanism, a nanoprobe-based fluorophore–quencher (Ab@afGQDs-MO) couple was employed for ferritin sensing. Using a dynamic linear range from 10 to 4000 ng·mL–1 with an R 2 value of 0.994, a limit of detection of 0.723 ng·mL–1 is achieved. With optimization of other input parameters, the ferritin is estimated in spiked serum samples as well.

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Fast ferritin immunoassay on PDMS microchips

Cited by 8 publications

References 48 publications

Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia

Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia

Microfluidic opportunities in the field of nutrition

Amine-Functionalized Graphene Quantum Dots for Fluorescence-Based Immunosensing of Ferritin

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