Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

Cheong, Jiyong; Yu, Hwanjo; Lee, Chang Yeol; Lee, Jung Uk; Choi, Hyun‐Jung; Lee, Jae Hyun; Lee, Hakho; Cheon, Jinwoo

doi:10.1038/s41551-020-00654-0

Cited by 168 publications

(150 citation statements)

References 53 publications

(52 reference statements)

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“…Very recently, new innovative technologies have been reported, with reliable efficiency in decreasing the overall time of the analysis and in pushing the limits of detection. Some of them are RT-PCR-like such as nano-PCR 25 , multiplex RT-PCR 26 , isothermal amplification 27 ; and others rely on the integration of interesting CRISPR-based detection 28 – 30 .…”

Section: Introductionmentioning

confidence: 99%

A simple and fast spectroscopy-based technique for Covid-19 diagnosis

Kitane

LOUKMAN²,

Marchoudi³

et al. 2021

Sci Rep

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The coronavirus pandemic, which appeared in Wuhan, China, in December 2019, rapidly spread all over the world in only a few weeks. Faster testing techniques requiring less resources are key in managing the pandemic, either to enable larger scale testing or even just provide developing countries with limited resources, particularly in Africa, means to perform tests to manage the crisis. Here, we report an unprecedented, rapid, reagent-free and easy-to-use screening spectroscopic method for the detection of SARS-CoV-2 on RNA extracts. This method, validated on clinical samples collected from 280 patients with quantitative predictive scores on both positive and negative samples, is based on a multivariate analysis of FTIR spectra of RNA extracts. This technique, in agreement with RT-PCR, achieves 97.8% accuracy, 97% sensitivity and 98.3% specificity while reducing the testing time post RNA extraction from hours to minutes. Furthermore, this technique can be used in several laboratories with limited resources.

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Section: Introductionmentioning

confidence: 99%

A simple and fast spectroscopy-based technique for Covid-19 diagnosis

Kitane

LOUKMAN²,

Marchoudi³

et al. 2021

Sci Rep

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“…So, a research team developed a fast-performing portable device named nano-PCR for COVID-19 detection in 17 minutes (using plasmonic heating through magneto-plasmonic nanoparticles). 45 Microscopic images of the magneto-plasmonic nanoparticles with a core/shell construction and also, a schematic of the nano-PCR assay to detect COVID-19, are presented in Fig. 10.…”

Section: The Role Of Iron-based Nanoparticles During the Covid-19 Crisismentioning

confidence: 99%

The latest strategies in the fight against the COVID-19 pandemic: the role of metal and metal oxide nanoparticles

2021

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“…[ 35 ] Another study reported the development of NPs with a magnetic core and a gold plasmonic shell. [ 36 ] Using such magneto‐plasmonic nanoparticles, a portable device was constructed to conduct rapid RT‐PCR amplification and fluorescence detection of viral RNAs from multiple samples within 17 minutes. The detection limit was comparable to standard RT‐PCR.…”

Section: Nanomaterials‐based Diagnostics For Tracking Sars‐cov‐2mentioning

confidence: 99%

Advances of nanomaterials‐based strategies for fighting against COVID‐19

Zeng

Hou

Bohmer

et al. 2021

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The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has infected over 100 million people globally due to its high infectivity. After decades of efforts on the studies of nanomaterials, researchers have applied nanomaterials‐based strategies to combat the pandemic of the coronavirus disease 2019 (COVID‐19). First, nanomaterials facilitate the development of easy, fast, and low‐cost diagnostic assays to detect SARS‐CoV‐2 and related biomarkers. Second, nanomaterials enable the efficient delivery of viral antigens to antigen‐presenting cells or serve as adjuvants in the host, leading to vaccine development at an unprecedented pace. Lastly, nanomaterials‐based treatments may inhibit SARS‐CoV‐2 replication and reduce inflammation. Overall, nanomaterials have played important roles in controlling this COVID‐19 pandemic. Here, we provide a brief overview of the representative examples of nanomaterials‐based diagnostics, vaccines, and therapeutics in the fight against COVID‐19.

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Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

Cited by 168 publications

References 53 publications

A simple and fast spectroscopy-based technique for Covid-19 diagnosis

A simple and fast spectroscopy-based technique for Covid-19 diagnosis

The latest strategies in the fight against the COVID-19 pandemic: the role of metal and metal oxide nanoparticles

Advances of nanomaterials‐based strategies for fighting against COVID‐19

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