Fast detection of coliform bacteria by means of gas chromatography-differential mobility spectrometry

Saptalena, Lena Ganda; Kuklya, Andriy; Telgheder, Ursula

doi:10.1007/s00216-016-9456-5

Cited by 2 publications

(3 citation statements)

References 30 publications

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“…In this study,4-methylumbelliferone was utilized as an indicator for the occurrence of E. coli. [41] In the favourable and unfavourable ion channels, detection limits of 4.511 ng (on column) were achieved for ONP. [42] These figures correlate to 3.37107 and 3.3107 E. coli mL À 1 cells.…”

Section: Emerging Techniques 41 Gas Chromatography-differential Mobil...mentioning

confidence: 99%

“…As a result, o‐nitrophenol was employed as an indicator for the existence of coliform (containing E. coli ). In this study,4‐methylumbelliferone was utilized as an indicator for the occurrence of E. coli [41] . In the favourable and unfavourable ion channels, detection limits of 4.511 ng (on column) were achieved for ONP [42] .…”

Section: Emerging Techniquesmentioning

confidence: 99%

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Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

et al. 2023

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Escherichia coli is a harmful pathogenic bacterial species causing serious intestinal sickness in humans. This bacterium has been linked to contaminated epidemics that have led to significant mortality and morbidity across the world. E. coli, like most other waterborne infections, is tricky to discover effectively in the water supply. Therefore, there is a demand for advanced E. coli detection methods that can sensitively and rapidly detect these pathogens. This review reveals several approaches used for the detection of E. coli bacteria using conventional methods such as multiple tube fermentation and membrane filtration techniques. The emerging approaches give quite accurate and speedy identification despite the necessity for culturing; nevertheless, they lack precision and necessitate extra lab testing. Because analytical techniques such as GC‐DMS lack specificity, the invention of a sensing device that is simple to use, compact, extremely sensitive, and specific has proven essential in identifying incredibly low concentrations of harmful E. coli in drinking water.

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Section: Emerging Techniques 41 Gas Chromatography-differential Mobil...mentioning

confidence: 99%

Section: Emerging Techniquesmentioning

confidence: 99%

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

et al. 2023

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“…At present, the commonly used methods are as follows: bacteria culture, enzyme‐linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), chromatography, and so on. [ 10–13 ] Bacteria culture, due to its many advantages such as simplicity, accuracy, and cheapness, had been widely used for counting total amounts of bacteria in water or food. ELISA is another bacteria detection method with the advantage of wide dynamic range, high sensitivity, and good specificity.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent

et al. 2020

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Bacterial pathogen control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) was synthesized using the oleylamine reducing method. It was found that Cu/Co/Ni TNA greatly enhanced the chemiluminescence (CL) signal of the hydroxylamine‐O‐sulfonic acid (HOSA)–luminol system. The CL properties of Cu/Co/Ni TNA were investigated systemically. The possible CL mechanism also was intensively investigated. Based on the enhanced CL phenomenon of Cu/Co/Ni TNA, a Cu/Co/Ni TNA, penicillin, and anti‐L. monocytogenes (Listeria monocytogenes) antibody‐based sandwich complex assay for detection of L. monocytogenes was established. In this sandwich CL assay, penicillin was employed to capture and enrich pathogenic bacteria with penicillin‐binding proteins (PBPs) while anti‐L. monocytogenes antibody was adopted as the specific recognition molecule to recognize L. monocytogenes. L. monocytogenes was detected sensitively based on this new Cu/Co/Ni TNA–HOSA–luminol CL system. The CL intensity was proportional to the L. monocytogenes concentration ranging from 2.0 × 102 CFU ml−1 to 3.0 × 107 CFU ml−1 and the limit of detection wa 70 CFU ml−1. The reliability and potential applications of our method was verified by comparison with official methods and recovery tests in environment and food samples.

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Fast detection of coliform bacteria by means of gas chromatography-differential mobility spectrometry

Cited by 2 publications

References 30 publications

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent

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