Fast detection of bacterial growth by using Portable Microbe Enrichment Unit (PMEU) and ChemPro100i® gas sensor

Hakalehto, Elias; Pesola, Jouni; Heitto, Anneli; Deo, Brajesh Bhanj; Rissanen, Kari; Sankilampi, Ulla; Humppi, Tarmo; Paakkanen, Heikki

doi:10.1016/j.pathophys.2009.03.001

Cited by 13 publications

(8 citation statements)

References 15 publications

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“…For example, septic contaminations by staphylococci, streptococci and enteric bacteria were verified in a few hours only on the basis of the volatiles emitted from the cultures in the PMEU Scentrion. 20 The effects of any antimicrobial substances could be studied also on the basis of the transmitted light or IR radiation in the PMEU Spectrion. 21 The effects of the peptide secretion (sample M3) on the growth curves of Staphylococcus aureus and Salmonella enterica Serovar typhimurium are presented in Figure 4.…”

Section: Biological Activity Studiesmentioning

confidence: 99%

Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria

et al. 2012

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A nano-HPLC-ESI-OrbiTrap study involving HCD and ETD spectra has been carried out to clarify the composition of the skin peptidome of brown Russian frogs Rana temporaria. This approach allowed determinantion of 76 individual peptides, increasing 3-fold the identified portion of the peptidome in comparison to that obtained earlier with FTICR MS. A search for the new bradykinin related peptides (BRPs) was carried out by reconstructing mass chromatograms based on the ion current of characteristic b- and y-ions. Several peptides were reported in the secretion of R. temporaria for the first time. The overall antibacterial activity of the skin secretion in general and of one individual peptide (Brevinin 1Tb) was determined using PMEU Spectrion (Portable Microbe Enrichment Unit) technology. The inhibitory effects of these peptides on Staphylococcus aureus and Salmonella enterica Serovar typhimutium were equal in scale to that reported for some antibiotics.

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Section: Biological Activity Studiesmentioning

confidence: 99%

Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria

et al. 2012

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“…This specific PMEU approach had been provisionally tested in a water distribution network system in Kuopio City, Finland (with 100.000 inhabitants and 350 km of pipelines) (Hakalehto et al, 2011c). Both hygienic indicators and various pathogens, such as Salmonellas, Campylobacteria, Yersinias, Staphylococci, and Bacillus cereus have been effectively monitored (Hakalehto, Pesola, Heitto, Närvänen, & Heitto, 2007;Hakalehto et al, 2009;Pitkänen et al, 2009;Mentu, Keitel, Heitto, & Hakalehto, 2009). For example, a single Salmonella cell produced a detectable signal (infrared light or ion mobility sensors or traditional medium plate confirmation) in about 10 hours (Hakalehto et al, 2011c).…”

Section: Pmeu In Water Microbiologymentioning

confidence: 99%

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Hakalehto¹,

Heitto²

2012

ENRR

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Water purity requires continuous monitoring. PMEU technology (Portable Microbe Enrichment Unit) provides the means for starting the enrichment and analysis immediately after the sampling. The entire procedure can be automated and microbe levels monitored in real-time from the enrichment broth by using IR, optical, UV or volatiles screening. For example, single cells of Escherichia coli have been verified from water samples in less than 10 hours in an official validation project. Also such pathogens as Salmonella, Campylobacter and Yersinia have been detected rapidly from natural, purified and irrigation waters. The PMEU technology has potential in the continuous monitoring within water departments and distribution systems, as well as in environmental studies.Keywords: rapid microbe detection, water hygiene, indicator bacteria, ColilertTM, PMEU, environmental monitoring, pathogens, water distribution BackgroundIn the present manuscript experimental approach and work carried out with the Portable Microbe Enrichment Unit (PMEU) is presented. This device is an ideal tool to investigate micro-organisms life at microscopic and molecular scale. Micro-organisms such as bacteria have a large influence on our lives with impact on economy, various industries, healthcare, agriculture and forestry, as well as in water management. In Finland our team published booklet entitled "Vesien Mikrobiaapinen" (in English: "A Microbiological ABC book of the waters") with the purpose to provide microbiological information to decision makers. As scientists we continuously deal with the public and their representatives: the political decision makers. In order to get funding, we ought to make them understand the importance of environmental protection and conservation of water sources, from the microbiological point of view.One area where micro-organisms have a large influence is the threat and deleterious impact on human and animal health caused by pathogenic micro-organisms. They are potentially distributed via waterways and often disseminated at small concentrations which still pose health hazards. In order to detect these minute concentrations, new tools are needed, also to simulate their biochemical activities. Their detection is not either a single or a simple event but a whole diagnostic process (Figure 1). Focusing on the often neglected step of specimen collection is of crucial importance. Sampling should be carried out considering all the physicochemical parameters, selection of sampling site etc. Usually it is required to transport and store the sample with caution and care. This is the step when most of the microbes present in the sample are often destroyed. They simply cannot survive or do not remain microbiologically viable. In order to avoid these problems, the Portable Microbe Enrichment Unit was developed. Time is saved in the PMEU processing of the samples, since the verification steps could follow sooner compared to traditional microbiology. The basic simple idea is to start the enrichment cultivation right subseq...

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“…Bacterial infections are the fifth highest cause of mortality in the United States, and their economic impact on the industrial, agricultural, and healthcare sector is significant [2][3][4][5]. However, despite their enhanced sensitivity and specificity, these methods are costly and time consuming and need to be performed by trained and experienced personnel [13][14][15][16][17][18]. Therefore, the need to develop fast, sensitive, and reliable detection methods for pathogenic bacteria in fields ranging from clinical diagnostics and monitoring of foodborne pathogens to detection of biological warfare agents is crucial in order to safeguard public health.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, other methods involving polymerase chain reaction and fluorescent immunoassays have been developed. However, despite their enhanced sensitivity and specificity, these methods are costly and time consuming and need to be performed by trained and experienced personnel .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and application of novel tricyanofuran hydrazone dyes as sensors for detection of microbes

Khattab

Gaffer

2016

Coloration Technology

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Acknowledging the need to develop rapid and sensitive bacterial recognition approaches, we functionalised the tricyanofuran hydrazone molecular switch. Of significant interest in relation to the synthesised hydrazones is the formation of two different conjugated structures upon exposure to different pH values. Many bacteria release ammonia gas, which alkalises environments. Herein we report the synthesis of a tricyanofuran hydrazone having the function of a colorimetric pH sensor. The UV-visible absorption and fluorescence spectra exhibit reversible colour changes of the tricyanofuran hydrazone solution in acetonitrile under acidbase conditions. Our results indicate that the tricyanofuran hydrazone probe can identify the bacterial targets quickly with high sensitivity. The infected samples exhibit a significant colour change from orange to blue and in the mean time there is a decrease in fluorescence emission as a function of ammonia and volatile amines released from bacterial metabolites. This tricyanofuran hydrazone chromophore is proposed for use in food packaging with a pH-sensing capability. Coloration TechnologySociety of Dyers and Colourists E T (30)solvent polarity parameter; eextinction coefficient; k max abs , nm -Maximum Absorption Wavelength; k max em , nm -Maximum Emission/ Fluorescence Wavelength.

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Fast detection of bacterial growth by using Portable Microbe Enrichment Unit (PMEU) and ChemPro100i® gas sensor

Cited by 13 publications

References 15 publications

Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria

Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Synthesis and application of novel tricyanofuran hydrazone dyes as sensors for detection of microbes

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