Fast cyclical-decellularized trachea as a natural 3D scaffold for organ engineering

Giraldo-Gómez, David M.; García-López, Sandra Julieta; Tamay-de-Dios, Lenin; Sánchez-Sánchez, Roberto; Villalba-Caloca, Jaime; Sotres-Vega, Avelina; Prado-Audelo, María Luisa Del; Gómez-Lizárraga, K.; Garciadiego-Cázares, David; Piña-Barba, María C.

doi:10.1016/j.msec.2019.110142

Cited by 34 publications

(43 citation statements)

References 65 publications

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“…With the successful application of tissue engineering decellularization technology in clinical tracheal transplantation cases, 5,9,15 methods to accelerate the preparation of tracheal acellular matrix are being continuously developed by scholars. 23–25 In this study, we investigated the effect increasing the DNase concentration in DEM on reducing the number of preparation cycles for rabbit acellular tracheal matrix and evaluated the changes in the main structure, immunogenicity, mechanical properties, ECM components, and biocompatibility of the tracheal matrix before and after acellularization. The experimental results showed that it took only 4 days from the acquisition of the trachea to final decellularized scaffold formation and good biocompatibility and mechanical properties of the tracheal matrix were maintained.…”

Section: Discussionmentioning

confidence: 99%

“…The immunological rejection of tracheal grafts has been the biggest obstacle to the success of tracheal replacement, and its immunogenicity is mainly derived from the epithelial and mucosal lamina propria. 25,27,28 Decellularization technology can effectively dissolve epithelial and mucosal cells in natural trachea through detergents and enzymes, remove the immunogenicity of the matrix, and eliminate the need for immunosuppressive agents to reduce rejection after surgery. 9,10 Studies have shown that nonchondral cells, such as those in the epithelium, interstitium, and muscle of the tracheal matrix of rabbits, are completely eliminated by seven DEM cycles, and MHC-II antigens are removed.…”

Section: Discussionmentioning

confidence: 99%

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Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

et al. 2020

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Objective: To shorten the preparation time of rabbit decellularized tracheal matrix through a modified detergent-enzymatic method with higher concentration of DNase (50 kU/mL), providing an experimental and theoretical basis for clinical decellularization technology. Methods: The control group was a natural trachea, and the experimental group was a tracheal matrix subjected to two and four decellularization cycles. The performance of each group of samples was evaluated by histology and immunohistochemical staining, scanning electron microscopy, biomechanical property testing, inoculation and cytotoxicity tests, and allograft experiments. Results: The results showed that the nuclei of the nonchondral areas of the tracheal stroma were essentially completely removed and MHC-I and MHC-II antigens were removed after two decellularization cycles. Histological staining and scanning electron microscopy showed that the extracellular matrix was retained and the basement membrane was intact. Cell inoculation and proliferation tests confirmed that the acellular tracheal matrix had good biocompatibility, and the proliferation capacity of bone mesenchymal stem cells on the matrix was increased in the experimental group compared with the control group ( p < 0.05). Histological staining and CD68 molecular marker analysis after the allograft experiment showed that the inflammatory response of the acellular tracheal matrix was weak and the infiltration of surrounding macrophages was reduced. Conclusion: A modified detergent-enzymatic method with an increased DNase (50 kU/mL) concentration requires only two cycles (4 days) to obtain a decellularized rabbit tracheal matrix with a short preparation time, good biocompatibility, suitable mechanical properties, and reduced preparation cost.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

et al. 2020

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“…• Sealing of tube can be difficult Vascular Niklason et al, 2001;Shen et al, 2003;L'Heureux et al, 2006;Pricci et al, 2009;Konig et al, 2009;Gauvin et al, 2010;Rayatpisheh et al, 2014;Jung et al, 2015;Gui et al, 2016;Zhao et al, 2018;Wang et al, 2018Intestine Grikscheit et al, 2002, 2004 • High degree of customization and control over scaffold production Xi-Xun et al, 2008;Yang et al, 2009;Neff et al, 2011;Lee et al, 2012Intestine Totonelli et al, 2012Trachea Johnson et al, 2016Butler et al, 2017;Ghorbani et al, 2017;Zhong et al, 2019;Batioglu-Karaaltin et al, 2019;Giraldo-Gomez et al, 2019;Wang et al, 2020 the properties and/or structure of the scaffold. The process of electrospinning, particularly for scaffold production, has been covered extensively in previous reviews (Pham et al, 2006;Rocco et al, 2014).…”

Section: Electrospinningmentioning

confidence: 99%

“…In recent years, research has been targeted toward improving in vitro preparation methods such as optimizing decellularization through enzymatic, detergent (Zhong et al, 2019), vacuum-assisted (Butler et al, 2017), and chemicalbased techniques (Batioglu-Karaaltin et al, 2019). For example, enhanced enzymatic approaches have drastically reduced tracheal decellularization time (Giraldo-Gomez et al, 2019;Wang et al, 2020). Importantly, the reduced preparation time had no adverse effects on tracheal ECM structure or biomechanical properties and evaded immunogenic or inflammatory responses when implanted in vivo.…”

Section: Tracheal Systemsmentioning

confidence: 99%

Building Scaffolds for Tubular Tissue Engineering

Boys

Barron

Tilev

et al. 2020

Front. Bioeng. Biotechnol.

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Hollow organs and tissue systems drive various functions in the body. Many of these hollow or tubular systems, such as vasculature, the intestines, and the trachea, are common targets for tissue engineering, given their relevance to numerous diseases and body functions. As the field of tissue engineering has developed, numerous benchtop models have been produced as platforms for basic science and drug testing. Production of tubular scaffolds for different tissue engineering applications possesses many commonalities, such as the necessity for producing an intact tubular opening and for formation of semi-permeable epithelia or endothelia. As such, the field has converged on a series of manufacturing techniques for producing these structures. In this review, we discuss some of the most common tissue engineered applications within the context of tubular tissues and the methods by which these structures can be produced. We provide an overview of the general structure and anatomy for these tissue systems along with a series of general design criteria for tubular tissue engineering. We categorize methods for manufacturing tubular scaffolds as follows: casting, electrospinning, rolling, 3D printing, and decellularization. We discuss state-of-the-art models within the context of vascular, intestinal, and tracheal tissue engineering. Finally, we conclude with a discussion of the future for these fields.

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“…This process implies the removal of the allogeneic or xenogeneic antigens from cells in the source tissue or organ that lead to an immune response in the host body (59,60). Thus, decellularization produces a natural three-dimensional scaffold containing the main elements of the ECM, which can be repopulated with host cells (61). One of the main advantages of decellularized matrices is the preservation of native architecture of the source tissue or organ in contrast to purified-component processes, e.g., we have obtained a human-decellularized artery with well-preserved elastin (Figure 3).…”

Section: Decellularized Cell Scaffolds Containing Elastinmentioning

confidence: 99%

Recent Advances in Elastin-Based Biomaterials

et al. 2020

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Elastin is one of the main components of the extracellular matrix; it provides resistance and elasticity to a variety of tissues and organs of the human body, besides participating in cellular signaling. On the other hand, elastin-derived peptides are synthetic biopolymers with a similar conformation and structure to elastin, but these possess the advantage of solubility in aqueous mediums. Due to their biological activities and physicochemical properties, elastin and related peptides may be applied as biomaterials to develop diverse biomedical devices, including scaffolds, hydrogels, and drug delivery systems for tissue engineering. Likewise, the combination of elastin with natural or synthetic polymers has demonstrated to improve the mechanical properties of biomedical products and drug delivery systems. Here we comprehensively describe the physicochemical properties and physiological functions of elastin. Moreover, we offer an overview of the use of elastin and its derivative polymers as biomaterials to develop scaffolds and hydrogels for tissue engineering. Finally, we discuss some perspectives on the employment of these biopolymers to fabricate new biomedical products.

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Fast cyclical-decellularized trachea as a natural 3D scaffold for organ engineering

Cited by 34 publications

References 65 publications

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

Building Scaffolds for Tubular Tissue Engineering

Recent Advances in Elastin-Based Biomaterials

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