"Fast-Breeding" in Apple: A Strategy to Accelerate Introgression of New Traits Into Elite Germplasm

Volz, Richard K.; Rikkerink, Erik H. A.; Austin, P.; Lawrence, T.; Bus, Vincent G. M.

doi:10.17660/actahortic.2009.814.20

Cited by 22 publications

(13 citation statements)

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“…Consequently, we used SSR markers to assess the amount of Evereste genome in each BC 0 1 seedling carrying the BpMADS4 transgene and the Fb_E locus. The proportion of SSR loci, whose alleles could be traced back unambiguously to one of the two grandparents, either Evereste or T1190, was 51% (40 out of 79), which is close to the proportion found by Volz et al (2009) in their SSR-based whole genome selection of the F1 progeny Royal Gala 9 A689-24 (65 out of 108, 60%). However, this percentage is lower than we expected considering that the majority of the SSR loci used here were reported to be polymorphic (63 out of 79) or even highly polymorphic (i.e.…”

Section: Acceleration Of the Introgression Of The Fb_e Locus From Evesupporting

confidence: 54%

“…Recently, optimal growing conditions in a controlled environment were utilized to accelerate the onset of flowering in apple, including high temperatures and relative humidity (26-30°C and 85%, respectively), high irradiance ([900 lmol m -2 s -1 ), long photoperiod (18 h), high red:far-red ratio, elevated CO 2 concentration (*1,800 ppm CO 2 ) and non-limiting fertigation ). None of these technical approaches was able to shorten the juvenile phase to less than 18 months Meilan 1997;Volz et al 2009). Alternative transgenic approaches have been tested to more drastically shorten the juvenile phase in apple.…”

Section: Introductionmentioning

confidence: 99%

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Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from cultivar Evereste

et al. 2011

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The long juvenile phase of Malus spp. has always been a major drawback for the rapid introgression of agronomically relevant traits (e.g. disease resistances) from wild apples into domestic apple cultivars (M. 9 domestica Borkh.). Several agrotechnical approaches have been investigated but none was able to reduce the juvenile phase to less than 18 months. Recently, an early flowering transgenic line named T1190 was obtained by over-expressing the BpMADS4 gene from silver birch (Betula pendula Roth.) in the apple cultivar Pinova. In this study, we report on the acceleration of the first two introgression cycles (F1 and BC 0 1) of the highly efficacious fire blight resistance locus Fb_E from the ornamental apple cultivar Evereste, using the BpMADS4-transgenic line T1190. A background selection based on simple sequence repeats (SSR) markers regularly distributed over the apple genome was applied to the 24 BC 0 1 seedlings carrying the BpMADS4 transgene and the Fb_E locus. Two early flowering BC 0 1 seedlings estimated to carry less than 15% of the genome of Evereste were identified. They are currently (July 2011) being used in reciprocal crosses with the apple cultivar Royal Gala to continue the introgression of the Fb_E locus. Additionally, the strong phenotypic effect of the Fb_E locus from Evereste was confirmed by artificially inoculating a T1190 9 Evereste F1 progeny with the causal agent of fire blight, Erwinia amylovora. Possible ways of enhancing the fast introgression of disease resistance genes in domestic apple using the transgenic line T1190 are discussed.

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Section: Acceleration Of the Introgression Of The Fb_e Locus From Evesupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from cultivar Evereste

et al. 2011

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“…This was the basis of a 'whole genome selection' strategy proposed by Volz et al (2009), which essentially maximises 'genomic similarity' of selection candidates to the recipient line while ensuring introgression of the gene of interest. They proposed the selection of seedlings from backcross families using a two-step approach.…”

Section: Gs For Disease-resistance Traitsmentioning

confidence: 99%

Towards genomic selection in apple (Malus × domestica Borkh.) breeding programmes: Prospects, challenges and strategies

Kumar

Bink

Volz

et al. 2011

Tree Genetics & Genomes

104

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The apple genome sequence and the availability of high-throughput genotyping technologies have initiated a new era where SNP markers are abundant across the whole genome. Genomic selection (GS) is a statistical approach that utilizes all available genome-wide markers simultaneously to estimate breeding values or total genetic values. For breeding programmes, GS is a promising alternative to the traditional marker-assisted selection for manipulating complex polygenic traits often controlled by many smalleffect genes. Various factors, such as genetic architecture of selection traits, population size and structure, genetic evaluation systems, density of SNP markers and extent of linkage disequilibrium, have been shown to be the key drivers of the accuracy of GS. In this paper, we provide an overview of the status of these aspects in current applebreeding programmes. Strategies for GS for fruit quality and disease resistance are discussed, and an update on an empirical genomic selection study in a New Zealand applebreeding programme is provided, along with a foresight of expected accuracy from such selection.

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“…The genetics of the Rvi16 scab resistance was studied on three families across two generations, where the first generation family (173 seedlings) was raised from seed of a 'Splendour'×MIS OP 93.051 G07-098 cross made in 1998. A 'fast-breeding' approach was applied to two selections from this family, AK617 and AK653, in order to accelerate flowering (Austin et al 2006;Volz et al 2009), and both were crossed in 2000 with 'Scired' to develop secondgeneration families consisting of 244 and 210 seedlings, respectively. Both 'Splendour' and 'Scired' are susceptible to apple scab.…”

Section: Plant Materials and Phenotypingmentioning

confidence: 99%

Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

Bus

Bassett

Bowatte

et al. 2010

Tree Genetics & Genomes

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Apple is host to a wide range of pests and diseases, with several of these, such as apple scab, powdery mildew and woolly apple aphid, being major causes of damage in most areas around the world. Resistance breeding is an effective way of controlling pests and diseases, provided that the resistance is durable. As the gene pyramiding strategy for increasing durability requires a sufficient supply of resistance genes with different modes of action, the identification and mapping of new resistance genes is an ongoing process in breeding. In this paper, we describe the mapping of an apple scab, a powdery mildew and a woolly apple aphid gene from progeny of openpollinated mildew immune selection. The scab resistance gene Rvi16 was identified in progeny 93.051 G07-098 and mapped to linkage group 3 of apple. The mildew and woolly aphid genes were identified in accession 93.051 G02-054. The woolly aphid resistance gene Er4 mapped to linkage group 7 to a region close to where previously the genes Sd1 and Sd2, for resistance to the rosy apple leaf-curling aphid, had been mapped. The mildew resistance gene Pl-m mapped to the same region on linkage group 11 where Pl2 had been mapped previously. Flanking markers useful for markerassisted selection have been identified for each gene.

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"Fast-Breeding" in Apple: A Strategy to Accelerate Introgression of New Traits Into Elite Germplasm

Cited by 22 publications

References 0 publications

Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from cultivar Evereste

Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from cultivar Evereste

Towards genomic selection in apple (Malus × domestica Borkh.) breeding programmes: Prospects, challenges and strategies

Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated Mildew Immune Selection

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