Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor

Gao, Rongke; Ko, Juhui; Cha, Kiweon; Jeon, Jun Ho; Rhie, Gi‐eun; Choi, Jonghoon; deMello, Andrew J.; Choo, Jaebum

doi:10.1016/j.bios.2015.05.005

Cited by 88 publications

(59 citation statements)

References 39 publications

(36 reference statements)

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“…The magnetic immunocomplexes were trapped by yoke-type-solenoids embedded on the microchannel where the SERS signals were measured. The assay estimated LOD was 100 pg/ml [186]. Instead of aggregating nanoparticles to create the SERS enhancement, other groups have developed microfluidic devices with nano-rough substrates already incorporated.…”

Section: Nanoparticle Aggregation and Sers Substrates In Microchannelsmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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Section: Nanoparticle Aggregation and Sers Substrates In Microchannelsmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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“…Many different types of biomarkers, including immunoglobulin (IgG) antigens [2,9,10], feline calicivirus (FCV) [5], prostate-specific antigen (PSA) [11], protein A [12], hepatitis B virus [13], and mucin protein (MUC4) [14] have been studied using this SERS-based assay platform. Recently, a rapid SERS-based assay technique using magnetic beads has been developed [15][16][17][18]. This method uses magnetic beads as antibody supporting materials, and the sandwich immunocomplexes are immobilized on the wall of a microtube using a magnetic bar.…”

Section: Introductionmentioning

confidence: 99%

Clinical validation of surface-enhanced Raman scattering-based immunoassays in the early diagnosis of rheumatoid arthritis

et al. 2015

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We assessed the clinical feasibility of conducting immunoassays based on surface-enhanced Raman scattering (SERS) in the early diagnosis of rheumatoid arthritis (RA). An autoantibody against citrullinated peptide (anti-CCP) was used as a biomarker, magnetic beads conjugated with CCP were used as substrates, and the SERS nanotags were comprised of anti-human IgG-conjugated hollow gold nanospheres (HGNs). We were able to determine the anti-CCP serum levels successfully by observing the distinctive Raman intensities corresponding to the SERS nanotags. At high concentrations of anti-CCP (>25 U/mL), the results obtained from the SERS assay confirmed those obtained via an ELISA-based assay. Nevertheless, quantitation via our SERS-based assay is significantly more accurate at low concentrations (<25 U/mL). In this study, we compared the results of an anti-CCP assay of 74 clinical blood samples obtained from the SERS-based assay to that of a commercial ELISA kit. The results of the anti-CCP-positive group (n = 31, >25 U/mL) revealed a good correlation between the ELISA and SERS-based assays. However, in the anti-CCP-negative group (n = 43, <25 U/mL), the SERS-based assay was shown to be more reproducible. Accordingly, we suggest that SERS-based assays are novel and potentially useful tools in the early diagnosis of RA.

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“…[9][10][11][12][13][14][15][16] Magnetic particles are easy to localize using a permanent magnet placed at the side of a vial, at the bottom of a wellplate, near a microfluidic channel, or alternatively using a controllable embedded solenoid. This provides a simple way to exchange buffers, automate assay steps on chip, and reversibly concentrate or aggregate ferric nano-and microparticles.…”

Section: Introductionmentioning

confidence: 99%

“…This provides a simple way to exchange buffers, automate assay steps on chip, and reversibly concentrate or aggregate ferric nano-and microparticles. For example, Jaebum Choo's group has utilized magnetic microbeads (analogous to commercially available TurboBeads) for a wide variety of SERS biosensing applications, including one embodiment using aptamer-coated gold nanoparticles (AuNPs) for detecting thrombin, 9 another using a solenoid microfluidic chip for trapping a AuNP competitive binding assay against the anthrax biomarker polyglutamic acid, 10 and an IgG immunoassay using antibody-coated hollow gold nanoparticles. 11 While these assays were successful and demonstrate the great potential in magneticbased SERS assays, they fail to capitalize on the surface enhancement of the magnetic bead.…”

Section: Introductionmentioning

confidence: 99%

Ferric plasmonic nanoparticles, aptamers, and magnetofluidic chips: toward the development of diagnostic surface-enhanced Raman spectroscopy assays

et al. 2016

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plasmonic nanoparticles, aptamers, and magnetofluidic chips: toward the development of diagnostic surface-enhanced Raman spectroscopy assays," J. Abstract. Conjugation of aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound nanoclusters that cause a measurable change in the colloid's optical properties. The optimization of a surface-enhanced Raman spectroscopy (SERS) competitive binding assay utilizing plasmonic "target" and magnetic "probe" nanoparticles for the detection of the toxin bisphenol-A (BPA) is presented. These assay nanoclusters were housed inside three types of optofluidic chips patterned with magnetically activated nickel pads, in either a straight or array pattern. Both Fe 2 O 3 and Fe 2 CoO 4 were compared as potential magnetic cores for the silver-coated probe nanoparticles. We found that the Ag@Fe 2 O 3 particles were, on average, more uniform in size and more stable than Ag@Fe 2 CoO 4 , whereas the addition of cobalt significantly improved the collection time of particles. Using Raman mapping of the assay housed within the magnetofluidic chips, it was determined that a 1 × 5 array of 50 μm square nickel pads provided the most uniform SERS enhancement of the assay (coefficient of variation ∼25%) within the magnetofluidic chip. Additionally, the packaged assay demonstrated the desired response to BPA, verifying the technology's potential to translate magnetic nanoparticle assays into a user-free optical analysis platform.

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Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor

Cited by 88 publications

References 39 publications

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Clinical validation of surface-enhanced Raman scattering-based immunoassays in the early diagnosis of rheumatoid arthritis

Ferric plasmonic nanoparticles, aptamers, and magnetofluidic chips: toward the development of diagnostic surface-enhanced Raman spectroscopy assays

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