Fast and Selective Extraction of Sulfonamides from Honey Based on Magnetic Molecularly Imprinted Polymer

Abstract

A fast and selective method was developed for the determination of sulfonamides (SAs) in honey based on magnetic molecularly imprinted polymer. The extraction was carried out by blending and stirring the sample, extraction solvent and polymers. When the extraction was complete, the polymers, along with the captured analytes, were easily separated from the sample matrix by an adscititious magnet. The analytes eluted from the polymers were determined by liquid chromatography-tandem mass spectrometry. Under the optimal conditions, the detection limits of SAs are in the range of 1.5-4.3 ng g(-1). The relative standard deviations of intra- and interday ranging from 3.7% to 7.9% and from 4.3% to 9.9% are obtained, respectively. The proposed method was successfully applied to determine SAs including sulfadiazine, sulfamerazine, sulfamethoxydiazine, sulfamonomethoxine, sulfadimethoxine, sulfamethoxazole and sulfaquinoxaline in different honey samples. The recoveries of SAs in these samples from 67.1% to 93.6% were obtained.

Synthesis and characterization of the core–shell magnetic molecularly imprinted polymers (Fe3O4@MIPs) adsorbents for effective extraction and determination of sulfonamides in the poultry feed

“…In comparison with antibodies, enzymes or biological receptors, MIPs show inherent advantages: their production is quite simple and economical, moreover the polymers show good physical and chemical stability and applicability in harsh chemical media without loss of binding properties. In recent years, several MIPs for sulfamethoxazole [19][20][21][22][23] and sulfamethazine [22][23][24][25][26][27][28] have been prepared as the stationary phase for HPLC and as solid-phase extracting agents, but few published articles has focused on analysis of sulfonamides in fish, pork, chicken and milk samples using methods based on MIP technology. Most MIPs for sulfonamides have been synthesized in bulk polymerization, followed by a grinding and sieving process to acquire the desired particles; these can suffer from drawbacks in some applications, for example, heterogeneous distribution of the binding sites, poor site accessibility to the target molecules because the template and functionality are totally embedded in the polymer matrices.…”

Photonic and magnetic dual responsive molecularly imprinted polymers: preparation, recognition characteristics and properties as a novel sorbent for caffeine in complicated samples

“…41,42 Compared to the two former methods usually involving complicated preparation processes and long times, suspension polymerization is simpler for preparing magnetic MIPs. So, herein, suspension polymerization was adopted to prepare DR-MIPs (Scheme 1(A)).…”

Emprego de polímeros de impressão molecular em preparo de amostras para análise de compostos orgânicos: aplicações e tendências

“…Ding et al, [115][116][117] em seus trabalhos, propuseram a síntese de polímeros magnéticos para a determinação de resíduos de fármacos em diferentes amostras. Para a determinação de fluoroquinolonas em amostras de água, foi sintetizado um MIP magnético para realizar a extração dos analitos e posterior análise por LC-MS. O ciprofloxacino foi utilizado como MM, além de MAA, EGDMA e Fe 3 O 4 como partícula magné-tica.…”

“…A extração foi feita sob agitação e após se completar, o polímero foi capturado e separado da matriz por atração magnética externa. Os extratos foram analisados por LC-MS e o método proposto permitiu a extração de sulfonamidas e seus análogos em diferentes amostras de mel [116] . Para a análise de tetraciclinas em amostras de tecido e ovo, um MIP magnético foi sintetizado usando oxitetraciclina como MM, MAA, estireno e divinilbenzeno como componente da matriz polimérica e Fe 3 O 4 como componente magnético.…”