Fast and quantitative determination of saxitoxin and neosaxitoxin in urine by ultra performance liquid chromatography-triple quadrupole mass spectrometry based on the cleanup of solid phase extraction with hydrophilic interaction mechanism

Xu, Xia; Huang, Baifen; Xu, Jiaojiao; Cai, Zengxuan; Zhang, Jing; Chen, Qing; Han, Jiaguang

doi:10.1016/j.jchromb.2017.11.032

Cited by 17 publications

(2 citation statements)

References 27 publications

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“…SOAE is a water based extract and hence it is expected to has polar components of SO. The hilic based HPLC column has been reported as best suitable column for the separation polar compounds [43–44], hence we have analyzed SOAE samples on HILIC (PerkinElmer, MA, USA) column, but it did not work well for the current application. Later, the separation of components tested on different types of reversed phase HPLC columns available in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Deme

Narasimhulu

Parthasarathy

2018

Journal of Chromatography B

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We previously reported that sesame oil (SO) has anti-inflammatory, anti-atherosclerotic and lipid lowering properties in vivo. Our recent studies have shown that, an aqueous extract of sesame oil (SOAE) has also anti-inflammatory and anti-atherosclerotic properties but with no lipid lowering effects. The extent of reduction in atherosclerosis led us to identify components of SOAE and evaluate their anti-inflammatory properties in vitro. Liquid chromatography mass spectrometric method was used to detect and identify components of SOAE. Methoxyphenol derivatives, short and long chain carboxylic acids, dicarboxylic acids, hydroxy and oxo- carboxylic acids were detected. To our surprise, sesamol and its derivatives (lignans), were not present in the SOAE. Among the identified, a combination of methoxy phenol compounds were selected and tested their ability to reduce LPS induced inflammatory gene expression. Monocyte derived macrophages/RAW 264.7 macrophages were pre-treated with these compounds for 2 h, followed by LPS stimulation for 24 h and pro-inflammatory gene expressions were analyzed. These methoxyphenol derivatives showed potent anti-inflammatory properties. In conclusion, the anti-inflammatory molecules associated with SO may contribute the anti-inflammatory and anti-atherosclerotic properties. Also, our results shed light for the development of SOAE based non-pharmacological therapeutics, nutritional supplements and health products for various inflammatory diseases in the future.

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Section: Discussionmentioning

confidence: 99%

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Deme

Narasimhulu

Parthasarathy

2018

Journal of Chromatography B

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“…Structural characterization of the aflatoxins was performed using the information-dependent acquisition (IDA) method. [17][18][19][20] The IDA method with dynamic background subtraction (DBS) was configured to trigger a sensitive enhanced product ion (EPI) scan when the survey scan signal exceeded the defined criteria. [21][22][23] An EPI spectrum library was constructed and can be used for aflatoxin compound screening.…”

Section: Introductionmentioning

confidence: 99%

Quantification and confirmation of four aflatoxins using a LC–MS/MS QTRAP system in multiple reaction monitoring, enhanced product ion scan, and MS³ modes

Lv¹,

Wang²,

Ge³

et al. 2019

Eur J Mass Spectrom (Chichester)

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A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of aflatoxins in peanuts and its derivative products or bean sauce. Samples (5 g) were extracted with acetonitrile/water/formic acid (79:20:1, v/v). After centrifugation and dilution, the extracts were separated on a C18 analytical column by gradient elution (acetonitrile/0.2% formic acid) and analyzed by UPLC–MS/MS. External calibration was used for qualification. The developed multiple reaction monitoring–information-dependent acquisition–enhanced product ion method enabled quantification and confirmation of the analytes in a single run. Enhanced product ion mode was used for qualitative analysis, while multiple reaction monitoring mode was used for quantitative analysis. An in-house library was constructed for identification. Calibration curves showed good linearity with correlation coefficients (r) higher than 0.994. Limits of detection were determined to be below 0.26 µg kg−1 for most analytes. The recoveries for those substances were in the acceptable range of 80.2%–119.1%. A new LC–MS3 method was established for further confirmation. One pickled pepper peanut was found to contain aflatoxins B1, B2, and G1 with contents of 90.93, 26.64, and 1.92 µg kg−1, respectively.

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l‐Cysteine‐modified magnetic microspheres for extraction and quantification of saxitoxin in rat plasma with liquid chromatography and tandem mass spectrometry

Zhu

Yang

et al. 2020

J of Separation Science

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Saxitoxin, which is one of the most typical paralytic shellfish poisoning toxins, ranks the highest intoxication rate of marine biological poisoning cases globally. Efficient clean‐up and extraction of saxitoxin from complex biological matrices are imperative for the analysis and concentration monitoring of the toxin when correlative poisoning cases happen. Herein, l‐cysteine‐modified magnetic microspheres based on metal‐organic coordination were synthesized by a facile approach and applied for magnetic solid‐phase extraction of saxitoxin from rat plasma samples before liquid chromatography–tandem mass spectrometry detection. Parameters, including adsorbent amount, extraction time, desorption solution, and desorption time that could affect the extraction efficiency, were respectively investigated. The developed method demonstrated good linearity in the range of 5–300 ng/mL (R2 = 0.9985) with a limit of quantification of 5 ng/mL and a limit of detection of 0.5 ng/mL, acceptable accuracy. and precision of within‐run and between‐run.

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Fast and quantitative determination of saxitoxin and neosaxitoxin in urine by ultra performance liquid chromatography-triple quadrupole mass spectrometry based on the cleanup of solid phase extraction with hydrophilic interaction mechanism

Cited by 17 publications

References 27 publications

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Quantification and confirmation of four aflatoxins using a LC–MS/MS QTRAP system in multiple reaction monitoring, enhanced product ion scan, and MS³ modes

l‐Cysteine‐modified magnetic microspheres for extraction and quantification of saxitoxin in rat plasma with liquid chromatography and tandem mass spectrometry

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Fast and quantitative determination of saxitoxin and neosaxitoxin in urine by ultra performance liquid chromatography-triple quadrupole mass spectrometry based on the cleanup of solid phase extraction with hydrophilic interaction mechanism

Cited by 17 publications

References 27 publications

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Identification and evaluation of anti-inflammatory properties of aqueous components extracted from sesame ( Sesamum indicum ) oil

Quantification and confirmation of four aflatoxins using a LC–MS/MS QTRAP system in multiple reaction monitoring, enhanced product ion scan, and MS3 modes

l‐Cysteine‐modified magnetic microspheres for extraction and quantification of saxitoxin in rat plasma with liquid chromatography and tandem mass spectrometry

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Quantification and confirmation of four aflatoxins using a LC–MS/MS QTRAP system in multiple reaction monitoring, enhanced product ion scan, and MS³ modes