Fast and non–derivative method based on high–performance liquid chromatography–charged aerosol detection for the determination of fatty acids from Agastache rugosa (Fisch. et Mey.) O. Ktze. seeds

Abstract

This study utilised response surface methodology to optimise the conditions for the extraction of A. rugosa seeds oil (ARO). Single-factor experiment and response surface methodology (RSM) were performed to identify the extraction time, liquid-solid ratio and extraction temperature that provided the highest yield of ARO. The optimal extraction time, liquid-solid ratio and extraction temperature were 8 h, 4:1 mL/g and 55 °C. The fatty acids (FAs) content and oil yield obtained through the optimised impregnation-extraction process were 19.67 mg/g and 32.1%. These values matched well with the predicted values. Linolenic acid was identified to be the main active ingredient of ARO. The high-performance liquid chromatography-charged aerosol detection method presented here is fast and does not require derivatisation. Therefore, it could be used to quantitatively analyse the FAs present in ARO and applied to detect compounds with low or no ultraviolet response.

Agastache Species: A Comprehensive Review on Phytochemical Composition and Therapeutic Properties

“…Known under the popular name of Korean mint, A. rugosa is native to East Asian countries, including China, Japan, and Korea. A. rugosa has empirically been used to treat cholera, vomiting, miasma, and other gastrointestinal issues [ 2 , 5 , 6 ].…”