Fast and efficient extraction of DNA from meat and meat derived products using aqueous ionic liquid buffer systems

Ressmann, Anna K.; Garcia, Eric Gonzalez; Khlan, Diana; Gärtner, Peter; Mach, Robert L.; Krska, Rudolf; Brunner, Kurt; Bica, Katharina

doi:10.1039/c5nj00178a

Cited by 20 publications

(16 citation statements)

References 43 publications

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“…Two works by the group of Bica 306 , 307 reported the use of IL-based SLE to extract genetic material from food matrices, attempting the creation of less laborious, time saving, and cost-effective routes for food quality control. These works included maize- and meat-derived products, and extended screenings of the chemical structures of the ILs (e.g., imidazolium-, cholinium-, and guanidinium-based, among others) were cautiously conducted.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

et al. 2017

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Ionic liquids (ILs) have been proposed as promising media for the extraction and separation of bioactive compounds from the most diverse origins. This critical review offers a compilation on the main results achieved by the use of ionic-liquid-based processes in the extraction and separation/purification of a large range of bioactive compounds (including small organic extractable compounds from biomass, lipids, and other hydrophobic compounds, proteins, amino acids, nucleic acids, and pharmaceuticals). ILs have been studied as solvents, cosolvents, cosurfactants, electrolytes, and adjuvants, as well as used in the creation of IL-supported materials for separation purposes. The IL-based processes hitherto reported, such as IL-based solid–liquid extractions, IL-based liquid–liquid extractions, IL-modified materials, and IL-based crystallization approaches, are here reviewed and compared in terms of extraction and separation performance. The key accomplishments and future challenges to the field are discussed, with particular emphasis on the major lacunas found within the IL community dedicated to separation processes and by suggesting some steps to overcome the current limitations.

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Section: Nucleic Acidsmentioning

confidence: 99%

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

et al. 2017

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“…A set of eight ionic liquids with a choline or 1,3-dialkylimidazolium core structure and variable anions was chosen for this study (Table 1). The selection is based on our previous experience in the direct extraction of DNA, as choline-based ionic liquids were particularly suitable for the extraction of DNA from meat samples 13 . Moreover, choline-based ionic liquids with carboxylate cations are typically considered as environmentally benign and bio-derived ionic liquids with low toxicity 16,17 .…”

Section: Resultsmentioning

confidence: 99%

“…We determined the tolerable concentration of the ILs by adding them to the qPCR reactions in different concentrations. We tested three different buffer systems that were previously reported for similar applications to dissolve and dilute the ILs, namely tris(hydroxymethyl)aminomethane (Tris, 10 mM, pH 8.0); 2-(N-morpholino)ethanesulfonic acid (MES, 50 mM, pH 6.0); and sodium phosphate (50 mM, pH 8.5) 13 . Subsequently, we spiked qPCR reactions containing 10 4 copies of Enterococcus spp.…”

Section: Resultsmentioning

confidence: 99%

Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids

Martzy

Bica-Schröder

Pálvölgyi

et al. 2019

Sci Rep

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The extraction of nucleic acids from microorganisms for subsequent molecular diagnostic applications is still a tedious and time-consuming procedure. We developed a method for the rapid preparation of genomic DNA from bacteria based on hydrophilic ionic liquids (ILs). First, we tested eight ILs in different buffer systems for their inhibitory effects on quantitative PCR. The cell lysis potential of different IL/buffer combinations was assessed by application on Enterococcus faecalis as a model organism for Gram-positive bacteria. The two best ILs, choline hexanoate and 1-ethyl-3-methylimidazolium acetate, were compared with the reference enzymatic method and two commercial DNA extraction kits. All methods were evaluated on four Gram-positive and four Gram-negative bacterial species that are highly relevant for environmental, food, or clinical diagnostics. In comparison to the reference method, extraction yields of the IL-based procedure were within one order of magnitude for most of the strains. The final protocol for DNA extraction using the two ILs is very low-cost, avoids the use of hazardous chemicals and can be performed in five minutes on a simple heating block. This makes the method ideal for high sample throughput and offers the opportunity for DNA extraction from bacteria in resource-limited settings or even in the field.

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“…Using this approach, the samples are incubated with the ILs for several minutes at a given temperature and can be subsequently used for (isothermal) amplification reactions. For example, our group has recently developed an IL-based method to extract DNA from various types of meat [ 19 ]. Biodegradable choline hexanoate in sodium phosphate buffer was used for sample lysis, resulting in a high DNA yield.…”

Section: Case Study: Towards Implementing a Field-applicable Workflowmentioning

confidence: 99%

Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis

et al. 2019

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Molecular diagnostic tools in the field of food and water quality analysis are becoming increasingly widespread. Usually, based on DNA amplification techniques such as polymerase chain reaction (PCR), these methods are highly sensitive and versatile but require well-equipped laboratories and trained personnel. To reduce analysis time and avoid expensive equipment, isothermal DNA amplification methods for detecting various target organisms have been developed. However, to make molecular diagnostics suitable for low-resource settings and in-field applications, it is crucial to continuously adapt the working steps associated with DNA amplification, namely sample preparation, DNA extraction, and visualization of the results. Many novel approaches have been evaluated in recent years to tackle these challenges, e.g., the use of ionic liquids for the rapid isolation of nucleic acids from organisms relevant for food and water analysis or the integration of entire analytical workflows on microfluidic chips. In any event, the future of applications in the field of isothermal amplification will probably lie in ready-to-use cartridges combined with affordable handheld devices for on-site analysis. This trend article aims to make prospective users more familiar with this technology and its potential for moving molecular diagnostics from the laboratory to the field. Graphical abstract

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Fast and efficient extraction of DNA from meat and meat derived products using aqueous ionic liquid buffer systems

Abstract: We present a fast and efficient strategy for DNA extraction from meat based on aqueous-ionic liquid systems that could extract DNA in significantly higher yields compared to the pure phosphate buffer.

Cited by 20 publications

References 43 publications

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids

Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis

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