Fast and accurate identification by <scp>MALDI‐TOF</scp> of the zoonotic serovar E of <i>Vibrio vulnificus</i> linked to eel culture

Boonstra, Mirjam; Fouz, Belén; Gelderen, Betty van; Dalsgaard, Inger; Madsen, Lone; Jansson, Eva; Amaro, Carmen; Haenen, O.L.M.

doi:10.1111/jfd.13756

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…separately" as keywords in the Google Scholar search engine (Figure 2). The bar graph in Figure 2 (Haider et al, 2023) and Anguilliformes species (eels) (Boonstra et al, 2023), respectively. V. anguillarum, another important vibriosis causing bacterium, was identified from Dicentrarchus labrax (European sea bass) and Sparus aurata (sea bream) by MALDI-TOF MS with an average mass score of 2.12-2.50 (Kazazić et al, 2019b;Jansson et al, 2020;Mougin et al, 2021).…”

Section: Vibrio Spp and Other Fish Pathogensmentioning

confidence: 99%

“…Aquaculture, a rapidly growing sector, suffers tremendous financial losses every year due to fish deaths caused by disease outbreaks and treatment expenses (Woo & Bruno, 2011). There are numbers of gram-negative, halophilic, flagellated and facultative anaerobic Vibrio species in Vibrionaceae family such as V. parahaemolyticus, V. vulnificus and V. cholerae cause * Corresponding author E-mail address: tulaycagatay@akdeniz.edu.tr (İ. T. Çağatay) diarrheal disease, septicaemia and serious wound infections in humans (Malainine et al, 2013;Burbick et al, 2018;Boonstra et al, 2023). V. alginolyticus, V. anguillarum, V. parahaemolyticus, V. harveyi, V. splendidus and V. ordalii are also known to be the causative agent of Vibriosis that causes symptoms of haemorrhagic septicaemia in various marine fish and freshwater fish (salmon, rainbow trout) (Silva-Rubio et al, 2008) as well as shellfish, crustaceans and bivalves (Tanrikul, 2007;Mougin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

Gökdağ,

Çağatay

2024

Marine Science and Technology Bulletin

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Aquaculture is developing rapidly and plays an important role in providing animal protein to the world’s growing population. However, increasing mortality from bacterial disease outbreaks in important species poses a challenge to production progress in this sector. In order to reduce the impact of these diseases, rapid and accurate pathogen identification is essential for disease management, early detection and the continued health of aquaculture. The aim of this review is to summarise studies on the identification and diagnosis of Vibrio pathogens in aquatic organisms by MALTI-TOF MS (Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry), a rapid identification method based on protein profiling of bacteria. The profiles of bacterial protein obtained are compared with a global microbial protein library for identification. This study demonstrates the potential of using MALDI-TOF MS for the detection of Vibrio pathogens in aquaculture in studies published between 2015 and 2024. While purchasing a time-of-flight mass spectrometer is expensive when compared to conventional and molecular identification methods. It also appears to be much more efficient in terms of time spent on identification. MALDI-TOF MS has been shown to be simple to use in fish identification laboratories.

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Section: Vibrio Spp and Other Fish Pathogensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

Gökdağ,

Çağatay

2024

Marine Science and Technology Bulletin

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“…If antibiotic therapies are delayed, the risk of death from the infection significantly increases (7,8). While many effective detection methods targeting this pathogen have been designed, most of these methods are time-consuming, complicated, or expensive (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

The Usage of an AMCA-Modified Aptamer Facilitates the Rapid Detection of Vibrio vulnificus

Cai,

Lian,

Liu

et al. 2024

Jundishapur J Microbiol

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Background: Vibrio vulnificus can cause serious infections in human beings associated with the consumption of raw oysters or cuts exposed to seawater. The traditional method for culturing V. vulnificus is time-consuming and has a high failure rate. Objectives: This study aims to detect V. vulnificus using an AMCA-modified specific DNA aptamer. Methods: Common pathogenic microorganisms present in the seawater of the Fujian Sea area were collected, cultured, and identified. The samples were found to contain mainly V. vulnificus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, V. alginolyticus, V. parahaemolyticus, and V. cholerae. AMCA was conjugated with 5’ ends of the aptamer using N-hydroxysuccinimide ester (NHS ester) to target V. vulnificus and produce a fluorescent signal upon binding. The aptamer was screened and optimized for rapid detection of V. vulnificus. We collected the 50 bacterial strains isolated from clinical secretion samples and used a fluorescence microscope to determine whether the sample contained V. vulnificus or not. We compared these results with those obtained from VITEK MS (considered the gold standard) to test the sensitivity and specificity of the AMCA-modified aptamer using IBM SPSS Statistics 22. Results: In this experiment, the sensitivity and specificity of the modified aptamer for detecting V. vulnificus were determined to be 100% [95% CI (0.39, 1)] and 93.4% [95% CI (0.81, 0.98)], respectively. The positive predictive value was 57% [95% CI (0.20, 0.88)], and the negative predictive value was 100% [95% CI (0.89, 1)]. These findings indicate that V. vulnificus specimens can be rapidly detected via fluorescence reaction within 30 minutes. Conclusions: Our results suggest that this modified DNA aptamer has the potential to be used for diagnosing V. vulnificus. Further research is needed to explore the application of aptamers in pathogen infections.

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“…Currently, this pathogen is identified by detection of specific genes and their amplification by polymerase chain reaction (Roig et al, 2022;Sanjuán & Amaro, 2007; https://doi.org/10.26434/chemrxiv-2023-t63jq ORCID: https://orcid.org/0000-0002-9899-1409 Content not peer-reviewed by ChemRxiv. License: CC BY-NC 4.0 Wright et al, 1995), or by analysis of its total proteins by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight) mass spectrometry and comparison with reference patterns (Dieckmann et al, 2010;Boonstra et al, 2023). All these methodologies require centralized laboratories with expensive and heavy equipment and trained personnel, which limits their accessibility in developing countries.…”

Section: Introductionmentioning

confidence: 99%

Vibrio vulnificus marine pathogen detection with thin-film impedance biosensors

Roig

Ibarlucea

Amaro

et al. 2023

Preprint

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Vibrio vulnificus (Vv) is a marine pathogen that can cause rapid death by septicemia (vibriosis) in humans and several fish species. This pathogen is considered a biomarker of climate change, as both its presence and vibriosis incidence in coastal environments are increasing because of global warming. Currently, gold-standard methods for Vv detection are all PCR-based, requiring expensive equipment and skilled personnel, which hinders their use on a global scale. The aim of this work was to design and test a more affordable method that could be used worldwide for both vibriosis diagnosis and pathogen monitoring in water. To this end, we functionalized thin film microelectrodes with thiolated single-stranded DNA sequences complementary to the species-specific genetic marker, the gene vvha, and monitored the impedance changes upon hybridization. We tested the biosensor specificity with synthetic and natural DNA samples (from cultures of Vv and V. cholerae, a closely related species) and determined the detectable concentration range. The results obtained showed that this biosensor was specific for Vv, achieving detection down to 1 pM DNA, which corresponds approximately to 3x102 bacteria/mL. Consequently, this biosensor could be used on a global scale for vibriosis diagnostics, health risk studies and climate change monitoring, with potential application for in situ detection.

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Fast and accurate identification by MALDI‐TOF of the zoonotic serovar E of Vibrio vulnificus linked to eel culture

Cited by 5 publications

References 42 publications

Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

The Usage of an AMCA-Modified Aptamer Facilitates the Rapid Detection of Vibrio vulnificus

Vibrio vulnificus marine pathogen detection with thin-film impedance biosensors

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