Fasciola hepatica : Specificity of a coproantigen ELISA test for diagnosis of fasciolosis in faecal samples from cattle and sheep concurrently infected with gastrointestinal nematodes, coccidians and/or rumen flukes (paramphistomes), under field conditions

Kajugu, P.-E.; Hanna, R.E.B.; Edgar, H.W.J.; McMahon, Connor; Cooper, M. G.; Gordon, Alan; Barley, J.P.; Malone, F.E.; Brennan, Gerard; Fairweather, Ian

doi:10.1016/j.vetpar.2015.07.018

Cited by 31 publications

(20 citation statements)

References 34 publications

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“…Fluke eggs were differentiated via the golden and clear colour of F. hepatica and C. daubneyi eggs, respectively (Kajugu et al. 2015 ), before the numbers of eggs counted were divided with the weight of feces tested to calculate the eggs per gram (EPG) values.…”

Section: Methodsmentioning

confidence: 99%

Rumen fluke (Calicophoron daubneyi) on Welsh farms: prevalence, risk factors and observations on co-infection withFasciola hepatica

et al. 2016

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SUMMARYReports of Calicophoron daubneyi infecting livestock in Europe have increased substantially over the past decade; however, there has not been an estimate of its farm level prevalence and associated risk factors in the UK. Here, the prevalence of C. daubneyi across 100 participating Welsh farms was recorded, with climate, environmental and management factors attained for each farm and used to create logistic regression models explaining its prevalence. Sixty-one per cent of farms studied were positive for C. daubneyi, with herd-level prevalence for cattle (59%) significantly higher compared with flock-level prevalence for sheep (42%, P = 0·029). Co-infection between C. daubneyi and Fasciola hepatica was observed on 46% of farms; however, a significant negative correlation was recorded in the intensity of infection between each parasite within cattle herds (rho = −0·358, P = 0·007). Final models showed sunshine hours, herd size, treatment regularity against F. hepatica, the presence of streams and bog habitats, and Ollerenshaw index values as significant positive predictors for C. daubneyi (P < 0·05). The results raise intriguing questions regarding C. daubneyi epidemiology, potential competition with F. hepatica and the role of climate change in C. daubneyi establishment and its future within the UK.

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Section: Methodsmentioning

confidence: 99%

Rumen fluke (Calicophoron daubneyi) on Welsh farms: prevalence, risk factors and observations on co-infection withFasciola hepatica

et al. 2016

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“…The MM3-COPRO test, based on the MM3 MoAb that binds to both CatL1 and CatL2 proteases (Mezo, Gonz alez-Warleta, Carro, & Ubeira, 2004), is commercialized by BIO X Diagnostics (La Jemelle, Belgium). Kajugu et al (2012Kajugu et al ( , 2015 showed that this test has a high diagnostic specificity (100%), and showed no cross reaction when authors showed the higher sensitivity of the molecular technique.…”

Section: Diagnosismentioning

confidence: 96%

“…Kajugu et al. (, ) showed that this test has a high diagnostic specificity (100%), and showed no cross reaction when tested with soluble fractions of homogenates from Paramphistomum cervi and Taenia hydatigena but also in co‐infections with paramphistome, coccidian and/or gastrointestinal nematodes. However, the sensitivity of this ELISA can sometimes be compromised by the high variability in the concentration of cathepsin proteinases in faecal samples and by differences in the between‐batch performance of peroxidase‐labelled anti‐mouse IgG polyclonal antibodies (Martínez‐Sernández, Orbegozo‐Medina, González‐Warleta, Mezo, & Ubeira, ).…”

Section: Diagnosismentioning

confidence: 99%

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Beesley

Caminade

Charlier

et al. 2017

Transbound Emerg Dis

157

111

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Summary Fasciola hepatica is a trematode parasite with a global distribution, which is responsible for considerable disease and production losses in a range of food producing species. It is also identified by WHO as a re‐emerging neglected tropical disease associated with endemic and epidemic outbreaks of disease in human populations. In Europe, F. hepatica is mostly associated with disease in sheep, cattle and goats. This study reviews the most recent advances in our understanding of the transmission, diagnosis, epidemiology and the economic impact of fasciolosis. We also focus on the impact of the spread of resistance to anthelmintics used to control F. hepatica and consider how vaccines might be developed and applied in the context of the immune‐modulation driven by the parasite. Several major research gaps are identified which, when addressed, will contribute to providing focussed and where possible, bespoke, advice for farmers on how to integrate stock management and diagnosis with vaccination and/or targeted treatment to more effectively control the parasite in the face of increasing the prevalence of infection and spread of anthelmintic resistance that are likely to be exacerbated by climate change.

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“…A coproantigen detection ELISA, based on a monoclonal antibody to fluke cathepsins (Mezo and others 2004), is offered by several diagnostic laboratories. Validation studies in naturally infected sheep suggest the coproantigen ELISA detects infection at a similar time to FECs (Gordon and others 2012) and, importantly, there is no evidence that it cross reacts with Calicophoron daubneyi , the rumen fluke, which is increasingly common in sheep and cattle in the UK (Kajugu and others 2015). The coproantigen ELISA can be used for individual sheep, but there are few data to support its use for composite samples (Flanagan and others 2011).…”

Section: Diagnosismentioning

confidence: 99%

Update on liver fluke in sheep

Williams¹

2020

In Practice

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Background:Fasciola hepatica is a common parasite of grazing livestock, which causes considerable economic losses through high levels of morbidity, mortality and reduced productivity. Its prevalence has increased in recent years, probably due to environmental changes.Aim of the article: This article focuses on sheep, highlighting some current issues and offering new insights into how fasciolosis can be better managed in this species.

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Fasciola hepatica : Specificity of a coproantigen ELISA test for diagnosis of fasciolosis in faecal samples from cattle and sheep concurrently infected with gastrointestinal nematodes, coccidians and/or rumen flukes (paramphistomes), under field conditions

Cited by 31 publications

References 34 publications

Rumen fluke (Calicophoron daubneyi) on Welsh farms: prevalence, risk factors and observations on co-infection withFasciola hepatica

Rumen fluke (Calicophoron daubneyi) on Welsh farms: prevalence, risk factors and observations on co-infection withFasciola hepatica

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Update on liver fluke in sheep

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