Fasciola hepatica in Snails Collected from Water-Dropwort Fields using PCR

Kim, Hwang‐Yong; Choi, In-Wook; Kim, Yeon-Rok; Quan, Juan-Hua; Ismail, Hassan Ahmed Hassan Ahmed; Cha, Guang-Ho; Hong, Sung-Jong; Lee, Young-Ha

doi:10.3347/kjp.2014.52.6.645

Cited by 9 publications

(10 citation statements)

References 14 publications

(35 reference statements)

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“…In Korea, the prevalence of F. hepatica infection was high in cattle before 2000 [ 15 ]. The infestations are still reported as PCR diagnosis of F. hepatica DNA from the intermediate host snails [ 16 , 17 ]. However, F. hepatica infection in wild animals is rarely identified.…”

Section: Discussionmentioning

confidence: 99%

A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea

et al. 2018

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A total of 44 adult or juvenile nutrias were necropsied for disease survey. A large nodule was found in the liver of a nutria. The histopathological specimen of the hepatic nodule was microscopically examined, and sectional worms were found in the bile duct. The worms showed a tegument with spines, highly branches of vitelline glands and intestine. Finally, we histopathologically confirmed fascioliasis in a wild nutria. In the present study, a case of fascioliasis in a wild nutria is first confirmed in Korea.

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Section: Discussionmentioning

confidence: 99%

A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea

et al. 2018

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“…However, F. hepatica -infected snails were not found at Hoenggye-ri (upper stream), which is located at the upper part of large cattle or sheep farms. The current infection status (1.5%) was lower than the previously reported values of 3.4% in Korea [8], 13.0% in Sweden [12], 7.0% in Switzerland [4], and 26.6% in Poland [5]. In France, the prevalence of natural F. hepatica infection was 1.1% for Lymnaea truncatula and 0.3% for Lymnaea glabra [14].…”

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confidence: 72%

Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea

et al. 2017

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Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 402 samples, F. hepatica 1TS-2 marker was detected in 6 freshwater snails; thus, the overall prevalence in freshwater snails was 1.5%. The prevalence varied between collection areas, ranging from 0.0% at Hoenggye-ri to 2.9% at Suha-ri. However, F. gigantica ITS-2 was not detected in the 6 F. hepatica-positive samples by PCR. The nucleotide sequences of the 6 F. hepatica ITS-2 PCR-positive samples were 99.4% identical to the F. hepatica ITS-2 sequences in GenBank, whereas they were 98.4% similar to F. gigantica ITS-2 sequences. These results indicated that the prevalence of F. hepatica in snail intermediate hosts was 1.5% in Gangwon-do, Korea; however the prevalence varied between collection areas. These results may help us to understand F. hepatica infection status in natural environments.

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“…Highly sensitive PCR tests has been reported for the identification of Fasciola infection in intermediate hosts. 20,21 Recently, Martinez-Valladares and Rojo-Vasquez reported on a conventional PCR test and a LAMP test that showed a LOD of 10 pg/μL of Fasciola DNA and detected 100% of the infections in sheep with a mean 84.4 (±54) eggs/g of stool. 22 Few studies have validated PCR tests to diagnose Fasciola using a large number of human clinical stool samples.…”

Section: Discussionmentioning

confidence: 99%

Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool

Cabada¹,

Malaga²,

Castellanos-González³

et al. 2016

Am J Trop Med Hyg

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is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively. The RPA adapted to a lateral flow platform was more sensitive than gel-based detection of the reaction products. In conclusion, the RPA is a highly sensitive and specific test to diagnose chronic infection using stool samples. The RPA lateral flow has the potential for deployment to endemic areas after further characterization.

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Fasciola hepatica in Snails Collected from Water-Dropwort Fields using PCR

Cited by 9 publications

References 14 publications

A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea

A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea

Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea

Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool

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