Fasciola hepatica: Effects on the antioxidative properties and lipid peroxidation of rat serum

Kołodziejczyk, Lidia; Siemieniuk, Ewa; Skrzydlewska, Elżbieta

doi:10.1016/j.exppara.2005.12.005

Cited by 42 publications

(31 citation statements)

References 44 publications

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“…However, even small changes in oxidant and/or antioxidant levels might disturb that balance. Many parasitic infections, whether single or combined, enhanced reactive oxygen species (ROS) generation that served to combat infection, including superoxide radical, nitric oxide, and hydrogen peroxide (Kolodziejczyk et al 2006). Trichinellosis led to oxidative stress and triggers peroxides in the muscles of mice at different stages of infection (Hadas and Gustowska 1995).…”

Section: Discussionmentioning

confidence: 99%

Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats

Shalaby

Moghazy

Shalaby³

et al. 2010

Parasitol Res

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There is a considerable interest in developing new anthelmintic drugs including those from medicinal plants due to increasing evidence of parasitic resistance against present anthelmintic drugs and decreasing activity against encapsulated larval stages of parasites. This study was carried out to assess, for the first time, the effectiveness of methanolic extract of Balanites aegyptiaca (BAE) fruits against different stages (pre-adult, migrating larvae, and encysted larvae) of Trichinella spiralis in rats compared with commonly used anthelmintic albendazole. Oral administration of BAE at a dose of 1,000 mg/kg b.wt. for five successive days throughout the parasite life cycle led to a marked reduction of migrating and encysted larval rate by 81.7% and 61.7%, respectively, in the muscular tissue. This treatment was less effective against adults in the gut (47.8%). Albendazole treatment at a dose of 10 mg/kg b.wt. for five successive days resulted in a marked eradication of T. spiralis adult worms (94.4%) and less reduction of migrating and encysted larval infections of skeletal muscles (62.2% and 26.4%, respectively). BAE-treated groups showed marked decreases in serum-glucose levels, triglyceride concentrations, aspartate aminotransferase (AST), creatinine phosphokinase (CPK) activities, and lipid peroxide products (malondialdehyde, MDA) as well as an increase in glutathione level in both serum and muscular tissue compared to albendazole-treated- and infected-untreated groups. This result was confirmed by few numbers of living- and dead-encysted larvae and less destruction of the diaphragm and skeletal muscle tissues in BAE-treated groups compared to other treated groups. It can be concluded that the methanolic extract of B. aegyptiaca fruits has high effectiveness against parenteral stages of T. spiralis than albendazole. Albendazole is more effective against enteral stage of T. spiralis than the extract.

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Section: Discussionmentioning

confidence: 99%

Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats

Shalaby

Moghazy

Shalaby³

et al. 2010

Parasitol Res

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“…Since significant increase in their degradation products or precursors occurs (in particular, non-esterified fatty acids), phospholipase is suggested to have an elevated activity. Moreover, concentration of lipid peroxidation products, such as malondialdehyde, 4-hydroxyketonal and conjugated dienes in liver preparations increases, what is accompanied by lowered antioxidant enzymes activities in serum (Kolodziejczyk et al 2005, 2006; Kaya et al 2006). It appears to be the direct evidence for oxidative damage to hepatic lipids in F. hepatica -infected animals (Siemieniuk et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Effect of Fasciola hepatica proteins on the functioning of rat hepatocytes

Wesołowska

Gajewska²,

Smaga-Kozlowska

et al. 2011

Parasitol Res

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Fasciolosis is a hepatic parasitic infection that affects many mammal species and creates a great economic and veterinary problem. Molecular mechanisms of parasite–hepatocyte interactions have not been precisely characterized yet. Therefore, the aim of the study was to investigate alterations in the metabolic activity of rat liver cells exposed to Fasciola hepatica somatic proteins. Hepatocytes were incubated with 0–1 mg/ml of fluke's somatic proteins for various periods of time. Afterward, changes in hepatocytes metabolic activity were determined with MTT and enzyme leakage tests. Hepatocytes' capacity to synthesize albumin was also investigated. It was observed that protein concentration, as well as longevity of their action, influenced metabolic activity of rat liver cells. Diminution of hepatocytes survival rate, an increase in enzyme leakage and altered synthetic capacity after treatment with parasite's proteins were reported. It is concluded that somatic proteins of F. hepatica may play an important role in liver cell damaging.

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“…Lipid peroxidation, an oxidative degeneration of polyunsaturated fatty acids set into motion by free radicals, leads to formation of highly reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), which can bind covalently to proteins resulting in their structural modifications Kamanli et al, 2004;Januszewski, 2005). Much attention has been focused on the formation and role of MDAand HNE-protein adducts in toxic and disease states (Hartley et al, 1999;Khan et al, 1999Khan et al, , 2002Sampey et al, 2003;Kolodziejczyk et al, 2006). However, role of lipid peroxidation, especially the formation and linkage of MDA-and HNE-protein adducts in the induction of autoimmune diseases remain largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Oxidative and nitrosative stress in trichloroethene-mediated autoimmune response

et al. 2007

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Reactive oxygen and nitrogen species (RONS) are implicated in the pathogenesis of several autoimmune diseases. Also, increased lipid peroxidation and protein nitration are reported in systemic autoimmune diseases. Lipid peroxidation-derived aldehydes (LPDAs) such as malondialdehyde (MDA) and 4-hydroxynonenal (HNE) are highly reactive and bind proteins covalently, but their potential to elicit an autoimmune response and contribution to disease pathogenesis remain unclear. Similarly, nitration of protein could also contribute to disease pathogenesis. To assess the status of lipid peroxidation and/or RONS, autoimmune-prone female MRL +/+ mice (5-week old) were treated with trichloroethene (TCE), an environmental contaminant known to induce autoimmune response, for 48 weeks (0.5 mg/ml via drinking water), and formation of antibodies to LPDA-protein adducts was followed in the sera of control and TCE-treated mice. TCE treatment led to greater formation of both anti-MDA-and and-HNE-protein adduct antibodies and higher serum iNOS and nitrotyrosine levels. The increase in TCE-induced oxidative stress was associated with increases in anti-nuclear-, anti-ssDNA-and anti-dsDNA-antibodies. These findings suggest that TCE exposure not only leads to oxidative/nitrosative stress, but is also associated with induction/exacerbation of autoimmune response in MRL +/+ mice. Further interventional studies are needed to establish a causal role of RONS in TCE-mediated autoimmunity.

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Fasciola hepatica: Effects on the antioxidative properties and lipid peroxidation of rat serum

Cited by 42 publications

References 44 publications

Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats

Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats

Effect of Fasciola hepatica proteins on the functioning of rat hepatocytes

Oxidative and nitrosative stress in trichloroethene-mediated autoimmune response

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