Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis.

Abstract

Abstract. Immunodominant antigens of an approximate molecular mass of 27 kD were obtained from an excretory-secretory product of adult Fasciola gigantica by a continuous-elution method. An indirect ELISA using the antigens obtained by this relatively simple procedure was developed for detecting specific antibodies from patients infected with F. gigantica. Sera from patients with other parasitic infections, healthy volunteers, and cholangiocarcinoma were also analyzed. The sensitivity, specificity, and positive and negative predictive values for this ELISA using the fractionated antigens were 100%. The data indicated a possible correlation of antibodies to F. gigantica with cholangiocarcinoma.

Immunodetection of Fasciola gigantica Circulating Antigen in Sera of Infected Individuals for Laboratory Diagnosis of Human Fascioliasis

“…Several Fasciola antigens have been detected as circulating antigens in serum or as coproantigens in feces and were successfully used in immunodiagnosis of human fascioliasis (8,11,16,34,40). Of these, one antigenic component with an approximate molecular mass of 27 kDa was found to give a consistent reaction with human fascioliasis sera (14,18,(41)(42)(43). Of note, all serologic tests based on the 27-kDa antigen were developed and optimized with an emphasis on the detection of antibodies to F. hepatica (12,13,44,45).…”

Evaluation of Immunoglobulin G Subclass Antibodies against RecombinantFasciola giganticaCathepsin L1 in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Fasciolosis

“…Despite recent studies involving the diagnosis of human fasciolosis using native F. gigantica excretory-secretory antigen (20,26,27), native F. gigantica cysteine proteinase (35) or recombinant F. gigantica cathepsin L1 (rCTL1) (37), specific IgG subclass antibodies to F. gigantica rCTL1 antigens as targets for an ELISA have not been studied sufficiently and need further investigation. In the present study, we evaluated four IgG subclass antibodies (IgG1, IgG2, IgG3, and IgG4) against F. gigantica rCTL1 in a cystatin capture ELISA for the serodiagnosis of human fasciolosis.…”

“…Serological tests for the diagnosis of fasciolosis have been developed as standard assays using native F. hepatica cathepsin L1 (31,32,34,36) or recombinant cathepsin L1 as marker antigens (5, 7, 31) as well as selected B-cell epitopes from F. hepatica cathepsin L1 (7,8). The development of an enzymelinked immunosorbent assay (ELISA) for the diagnosis of human fasciolosis based on the detection of serum immunoglobulin G4 (IgG4) antibody reactive to native or recombinant F. hepatica cathepsin L1 showed excellent potential (31,32,36).Despite recent studies involving the diagnosis of human fasciolosis using native F. gigantica excretory-secretory antigen (20,26,27), native F. gigantica cysteine proteinase (35) or recombinant F. gigantica cathepsin L1 (rCTL1) (37), specific IgG subclass antibodies to F. gigantica rCTL1 antigens as targets for an ELISA have not been studied sufficiently and need further investigation. In the present study, we evaluated four IgG subclass antibodies (IgG1, IgG2, IgG3, and IgG4) against F. gigantica rCTL1 in a cystatin capture ELISA for the serodiagnosis of human fasciolosis.…”

Molecular Confirmation that Fasciola gigantica Can Undertake Aberrant Migrations in Human Hosts

“…The spontaneous emergence of the fluke from the knee was 4 months after the appearance of the first diagnostic signals in the liver and 1 month after the appearance of the lesion on the knee. Serum collected when the fluke emerged yielded a titer of 1:12,800 by a standard enzyme-linked immunosorbent assay protocol (20) with native excreted-secreted protein antigen produced from Fasciola gigantica (by use of a slight modification of a previously described method [4,11]). After the emergence of the worm, all clinical symptoms, on the knee and elsewhere, disappeared and the patient remains completely healthy.…”