Fascin promotes filopodia formation independent of its role in actin bundling

Zanet, Jennifer; Jayo, Asier; Plaza, Serge; Millard, Tom H.; Parsons, Maddy; Stramer, Brian

doi:10.1083/jcb.201110135

Cited by 86 publications

(130 citation statements)

References 35 publications

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“…6D,E; supplementary material Movies 10, 11). These results are consistent with a correlation between the activity, accumulation and phosphorylation state of Sn (Nagel et al, 2012;Zanet et al, 2012).…”

Section: S52asupporting

confidence: 79%

“…However, in in vivo systems such as Drosophila, phosphorylation on S52 inactivates sn activity in certain conditions but not in others (Zanet et al, 2009). Furthermore, although it is widely accepted that fascins act as actin-bundling proteins, a recent report proposed a bundling-independent activity of fascins that promote filopodia formation (Zanet et al, 2012).…”

Section: Molecular Requirements For Sn During Tracheal Formationmentioning

confidence: 99%

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Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis

Okenve-Ramos

Llimargas

2014

Development

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A key challenge in normal development and in disease is to elucidate the mechanisms of cell migration. Here we approach this question using the tracheal system of Drosophila as a model. Tracheal cell migration requires the Breathless/FGFR pathway; however, how the pathway induces migration remains poorly understood. We find that the Breathless pathway upregulates singed at the tip of tracheal branches, and that this regulation is functionally relevant. singed encodes Drosophila Fascin, which belongs to a conserved family of actin-bundling proteins involved in cancer progression and metastasis upon misregulation. We show that singed is required for filopodia stiffness and proper morphology of tracheal tip cells, defects that correlate with an abnormal actin organisation. We propose that singed-regulated filopodia and cell fronts are required for timely and guided branch migration and for terminal branching and branch fusion. We find that singed requirements rely on its actin-bundling activity controlled by phosphorylation, and that active Singed can promote tip cell features. Furthermore, we find that singed acts in concert with forked, another actin cross-linker. The absence of both cross-linkers further stresses the relevance of tip cell morphology and filopodia for tracheal development. In summary, our results on the one hand reveal a previously undescribed role for forked in the organisation of transient actin structures such as filopodia, and on the other hand identify singed as a new target of Breathless signal, establishing a link between guidance cues, the actin cytoskeleton and tracheal morphogenesis.

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Section: S52asupporting

confidence: 79%

Section: Molecular Requirements For Sn During Tracheal Formationmentioning

confidence: 99%

Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis

Okenve-Ramos

Llimargas

2014

Development

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“…Transcriptional profiling studies suggest that Drosophila Kc167 cells are plasmatocytes (Cherbas et al 2011), a subclass of hemocytes involved in immune surveillance that may further differentiate into lamellocytes, podocytes, or macrophages (Stofanko et al 2010). To examine whether ecdysone tRNA levels and protein translation www.rnajournal.org 1809 treatment has an effect on the differentiation state of Kc167 cells, we analyzed expression markers for these relevant cell types at 0, 3, and 48 h. Up-regulation of Croquemort, a macrophage receptor for apoptotic cells (Franc et al 1996(Franc et al , 1999, Singed (Fascin), an actin-bundling protein important for macrophage migration (Zanet et al 2012), and Schnurri, previously shown to regulate hemocyte-to-macrophage differentiation (Kelsey et al 2012), together suggest that ecdysone induces plasmatocyte-to-macrophage differentiation in this cell line (Fig. 3A).…”

Section: Transcriptional and Proteomic Response To Steroidhormone Sigmentioning

confidence: 99%

Integrated tRNA, transcript, and protein profiles in response to steroid hormone signaling

Bortle

Nichols

Ramos

et al. 2015

RNA

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The accurate and efficient transfer of genetic information into amino acid sequences is carried out through codon-anticodon interactions between mRNA and tRNA, respectively. In this way, tRNAs function at the interface between gene expression and protein synthesis. Whether tRNA levels are dynamically regulated and to what degree tRNA abundance influences the cellular proteome remains largely unexplored. Here we profile tRNA, transcript and protein levels in Drosophila Kc167 cells, a plasmatocyte cell line that, upon treatment with 20-hydroxyecdysone, differentiates into macrophages. We find that high abundance tRNAs associate with codons that are overrepresented in the Kc167 cell proteome, whereas tRNAs that are in low supply associate with codons that are underrepresented. Ecdysone-induced differentiation of Kc167 cells leads to changes in mRNA codon usage in a manner consistent with the developmental progression of the cell. At both early and late time points, ecdysone treatment concomitantly increases the abundance of tRNAThr(CGU), which decodes a differentiation-associated codon that becomes enriched in the macrophage proteome. These results together suggest that tRNA levels may provide a meaningful regulatory mechanism for defining the cellular proteomic landscape.

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“…For domain analysis, UAS-GFPmoesin (Dutta et al, 2002) or UASLifeActGFP (Zanet et al, 2012) was driven in haemocytes to label actin, and cells were visualised for 2 hours at 1-minute intervals. The time-lapse series was then thresholded within ImageJ (NIH) and flattened with an average intensity projection.…”

Section: Domain Analysismentioning

confidence: 99%

Emergence of embryonic pattern through contact inhibition of locomotion

et al. 2012

Self Cite

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SUMMARYThe pioneering cell biologist Michael Abercrombie first described the process of contact inhibition of locomotion more than 50 years ago when migrating fibroblasts were observed to rapidly change direction and migrate away upon collision. Since then, we have gleaned little understanding of how contact inhibition is regulated and only lately observed its occurrence in vivo. We recently revealed that Drosophila macrophages (haemocytes) require contact inhibition for their uniform embryonic dispersal. Here, to investigate the role that contact inhibition plays in the patterning of haemocyte movements, we have mathematically analysed and simulated their contact repulsion dynamics. Our data reveal that the final pattern of haemocyte distribution, and the details and timing of its formation, can be explained by contact inhibition dynamics within the geometry of the Drosophila embryo. This has implications for morphogenesis in general as it suggests that patterns can emerge, irrespective of external cues, when cells interact through simple rules of contact repulsion.

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Fascin promotes filopodia formation independent of its role in actin bundling

Abstract: Mutation of a critical residue of fascin eliminates the protein’s actin-bundling activity but maintains its positive role in filopodia formation

Cited by 86 publications

References 35 publications

Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis

Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis

Integrated tRNA, transcript, and protein profiles in response to steroid hormone signaling

Emergence of embryonic pattern through contact inhibition of locomotion

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