Fasciclin II and Beaten path modulate intercellular adhesion in<i>Drosophila</i>larval visual organ development

Holmes, Amy L.; Heilig, Joseph S.

doi:10.1242/dev.126.2.261

Cited by 17 publications

(1 citation statement)

References 73 publications

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“…Functionally, Beat has been implicated in axon defasciculation at choice points, whereas Side was shown to attract motor axons. Besides motor nerves, Beat is also involved in development of the Bolwig’s nerve and its target, the Bolwig’s organ, which together constitute the larval visual system ( Holmes and Heilig, 1999 ). Overexpression experiments in developing muscles confirmed that Beat has profound effects on the migration and defasciculation of axons ( Fambrough and Goodman, 1996 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into the axon guidance molecules Sidestep and Beaten path

et al. 2022

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The transmembrane protein Sidestep (Side) functions as a substrate-bound attractant for motor axons in Drosophila. Outgrowing motor axons recognize Side via Beaten path Ia (Beat) and migrate along Side-expressing tissues. Here, we report a structure-function analysis of these guidance molecules using a variety of mutant lines and transgenic constructs. Investigation of Side mutants shows that the exchange of a single amino acid (L241H) in the second immunoglobulin domain disturbs Side function and subcellular localization. Overexpression of Side and Beat deletion constructs in S2 cells and muscles demonstrate that the first Ig domains of both proteins are necessary for their interaction. Furthermore, subcellular distributions of several Beat constructs identify functional domains and suggest a potential posttranslational processing step in ER compartments. In fact, fusing full-length Beat at both the N- and C-terminus with GFP and mCherry, respectively, shows that the N-terminal domain is transported to the plasma membrane and exposed on the cell surface, while the C-terminal domain accumulated in the nucleus. Taken together, these results give insights into the interaction of Side and Beat and imply that Beat might be subject to proteolytic cleavage during maturation.

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Section: Introductionmentioning

confidence: 99%

Molecular insights into the axon guidance molecules Sidestep and Beaten path

et al. 2022

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N‐cadherin regulates primary motor axon growth and branching during zebrafish embryonic development

Brusés

2011

J of Comparative Neurology

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N-cadherin is a classical type I cadherin that contributes to the formation of neural circuits by regulating growth cone migration and the formation of synaptic contacts. This study analyzed the role of N-cadherin in primary motor axons growth during development of the zebrafish (Danio rerio) embryo. After exiting the spinal cord, primary motor axons migrate ventrally through a common pathway and form the first neuromuscular junction with the muscle pioneer cells located at the horizontal myoseptum, which serves as a choice point for cell-type specific pathway selection. Analysis of N-cadherin mutants (cdh2hi3644Tg) and embryos injected with N-cadherin antisense morpholinos showed primary motor axons extending aberrant axonal branches at the choice point in ~40% of the somitic hemisegments, and an ~150% increase in the number of branches per axon length within the ventral myotome. Analysis of individual axons trajectories showed that the caudal (CaP) and rostral (RoP) motor neurons axons formed aberrant branches at the choice point which abnormally extended in the rostrocaudal axis and ventrally to the horizontal myoseptum. Expression of a dominant-interfering N-cadherin cytoplasmic domain in primary motor neurons caused some axons to abnormally stall at the horizontal myoseptum and to impair their migration into the ventral myotome. However, in N-cadherin depleted embryos the majority of primary motor axons innervated their appropriate myotomal territories indicating that N-cadherin regulates motor axon growth and branching without severely affecting the mechanisms that control axonal target selection.

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Different Isoforms of Fasciclin II Are Expressed by a Subset of Developing Olfactory Receptor Neurons and by Olfactory-Nerve Glial Cells during Formation of Glomeruli in the Moth Manduca sexta

Higgins

Gibson

Eckholdt

et al. 2002

Developmental Biology

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During development of the primary olfactory projection, olfactory receptor axons must sort by odor specificity and seek particular sites in the brain in which to create odor-specific glomeruli. In the moth Manduca sexta, we showed previously that fasciclin II, a cell adhesion molecule in the immunoglobulin superfamily, is expressed by the axons of a subset of olfactory receptor neurons during development and that, in a specialized glia-rich "sorting zone," these axons segregate from nonfasciclin II-expressing axons before entering the neuropil of the glomerular layer. The segregation into fasciclin II-positive fascicles is dependent on the presence of the glial cells in the sorting zone. Here, we explore the expression patterns for different isoforms of Manduca fasciclin II in the developing olfactory system. We find that olfactory receptor axons express transmembrane fasciclin II during the period of axonal ingrowth and glomerulus development. Fascicles of TM-fasciclin II+ axons target certain glomeruli and avoid others, such as the sexually dimorphic glomeruli. These results suggest that TM-fasciclin II may play a role in the sorting and guidance of the axons. GPI-linked forms of fasciclin II are expressed weakly by glial cells associated with the receptor axons before they reach the sorting zone, but not by sorting-zone glia. GPI-fasciclin II may, therefore, be involved in axon-glia interactions related to stabilization of axons in the nerve, but probably not related to sorting.

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Fasciclin II and Beaten path modulate intercellular adhesion inDrosophilalarval visual organ development

Cited by 17 publications

References 73 publications

Molecular insights into the axon guidance molecules Sidestep and Beaten path

Molecular insights into the axon guidance molecules Sidestep and Beaten path

N‐cadherin regulates primary motor axon growth and branching during zebrafish embryonic development

Different Isoforms of Fasciclin II Are Expressed by a Subset of Developing Olfactory Receptor Neurons and by Olfactory-Nerve Glial Cells during Formation of Glomeruli in the Moth Manduca sexta

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