Fascia implantation with fibroblast growth factor on vocal fold paralysis

Evaluation of Autologous Fascia Implantation With Controlled Release of Fibroblast Growth Factor for Recurrent Laryngeal Nerve Paralysis Due to Long-term Denervation

“…An implantation bed was secured under the thyroid lamina in order to ablate around the connective tissue. The implanted materials were planted onto this bed, which was located on the lateral side of the thyroarytenoid (TA) muscle and lateral cricothyroid muscle 8 ( Figure 1).…”

“…The gelatin hydrogel was trimmed into 1 × 1 mm squares, which were then soaked in an aqueous solution containing 0.1 or 1 μg bFGF at room temperature 3 hours before use. 8 Rats were divided into the following 4 groups: a group implanted with fascia and gelatin sheet with 1 μg bFGF (1 μg bFGF + fascia group, n = 5), a group implanted with fascia and gelatin sheet with 0.1 μg bFGF (0.1 μg bFGF + fascia group, n = 5), a group implanted with fascia and gelatin sheet without bFGF (fascia group, n = 5), and a group without implantation (RLN section group, n = 5).…”

Preliminary results of tissue‐engineered injection laryngoplasty material in a rabbit model

“…Given these shortcomings, additional work to optimize injection material is warranted. Recent efforts to move toward a more ideal injectable have focused on either biological enhancement of traditional materials or use of long‐lasting synthetic biopolymers to enhance injection longevity . We hypothesized that we could create long‐lasting biomaterial using tissue‐engineering principles by utilizing a bioscaffold, a cell source, and growth factors (GFs) to create a novel, self‐sustaining biomaterial .…”

“…To promote chondrocytic differentiation, we used chondrocytic GFs in both the culture and injected solution. No previous studies have evaluated the effect of GFs on MACD; however, fibroblast GF has been shown to increase the longevity of fascia implanted into the larynx . We then tested this material using a rabbit model of unilateral vocal fold paralysis and performed IL with three experimental groups: MACD alone (group 1), MACD combined with undifferentiated ADSCs and GFs (group 2), and MACD combined with predifferentiated chondrocytic cells and GFs (group 3).…”

“…Recent efforts to move toward a more ideal injectable have focused on either biological enhancement of traditional materials or use of long-lasting synthetic biopolymers to enhance injection longevity. [5][6][7][8] We hypothesized that we could create long-lasting biomaterial using tissueengineering principles by utilizing a bioscaffold, a cell source, and growth factors (GFs) to create a novel, selfsustaining biomaterial. 9 Our aim was to create a material with cells that had a chondrocytic phenotype to allow for long-lasting medialization of the vocal cord via a transoral approach.…”