Farnesol-induced hyperbranched morphology with short hyphae and bulbous tips of Coriolus versicolor

Abstract

As the first fungal quorum sensing molecule, farnesol-induced morphological transition is usually studied in dimorphic fungi, but in basidiomycetes the morphological changes regulated by farnesol are rarely investigated. In this study, we found that farnesol made the basidiomycete Coriolus versicolor develop into a hyperbranched morphology with short hyphae and bulbous tips. Farnesol treatment resulted in a significant increase of intracellular oxidative stress level, which influenced the expression of several morphogenesis-related genes, and thereby led to the morphological changes. High oxidative stress level significantly stimulated the expression of laccase genes for improving intracellular laccase biosynthesis. The resulted hyperbranched morphology further accelerated the secretion of intracellular laccase into culture medium. As a result, extracellular laccase production reached a maximum of 2189.2 ± 54.7 U/L in farnesol-induced cultures, which was 6.8-fold greater than that of control cultures. SDS-PAGE and native-PAGE showed that farnesol increased laccase production by promoting the biosynthesis of three laccase isoforms. Together these results provide new opportunities in not only understanding the farnesol-regulated mycelial morphology in basidiomycetes, but also developing novel strategies for enhancing the production of secreted enzymes of biotechnological interest.

Linoleic acid functions as a quorum‐sensing molecule in Monascus purpureus–Saccharomyces cerevisiae co‐culture

“…Some of these inductive molecules are quorum‐sensing molecules that play various roles in the development and metabolism of Monascus (Erkaya et al, 2020; Z. R. Huang et al, 2018; Shi et al, 2022). Quorum‐sensing molecules are related to cell density and regulate morphology and secondary metabolite production in a variety of filamentous fungi (Palonen et al, 2017a, 2017b; Raina et al, 2010; Sorrentino et al, 2010; Wang et al, 2018; Williams et al, 2012).…”

“…Quorum-sensing molecules are related to cell density and regulate morphology and secondary metabolite production in a variety of filamentous fungi (Palonen et al, 2017a(Palonen et al, , 2017bRaina et al, 2010;Sorrentino et al, 2010;Wang et al, 2018;Williams et al, 2012). Some quorum-sensing molecules are not only self-induced and trigger intracellular communication but can also be induced heterogeneously and play a vital role in intercellular communication.…”

Potential Use of Microbial Enzymes for the Conversion of Plastic Waste Into Value-Added Products: A Viable Solution

“…Contrary to popular belief, different fungal consortia have been proven to destroy various polymers, such as PET and PLA, through synergistic action ( Ghosh et al, 2019 ; Nikolaivits et al, 2021 ). Before eventual depolymerization, as well as their potential to release hydrophobin for improved hyphal adhesion to hydrophobic substrates, fungal hyphae are critical in both the initial colonization and the subsequent depolymerization of the fungus ( Wang et al, 2018 ; Quarantin et al, 2019 ). Pretreatment factors such as exposure to light and temperature and acid pretreatment and various additives have been used to enhance fungal biodegradation of plastics ( Montazer et al, 2020 ).…”