Farnesoic acid O‐methyl transferase (FAMeT) isoforms: Conserved traits and gene expression patterns related to caste differentiation in the stingless bee, <i>Melipona scutellaris</i>

Vieira, Carlos Ueira; Bonetti, Ana Maria; Simões, Zilá Luz Paulino; Maranhão, Andréa Queiroz; Costa, Christiane S.; Costa, María; Siquieroli, Ana Carolina Silva; Nunes, Francis Morais Franco

doi:10.1002/arch.20224

Cited by 24 publications

(25 citation statements)

References 26 publications

(29 reference statements)

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“…In this study, the production of MF was shown to increase in relation to increasing amounts of recombinant FAMeT in a radiochemical assay (Gunawardene et al, 2002). However, subsequent studies in other Crustacea (Litopenaeus vannamei, Homarus americanus, Cancer pagurus) and insect species (Ceratitis capitata, Nilaparvata lugens and Melipona scutellaris) did not verify this activity (Holford et al, 2004;Hui et al, 2008;Liu et al, 2010;Ruddell et al, 2003;Vannini et al, 2010;Vieira et al, 2008). Moreover, two recent studies in D. melanogaster have shown that DmFAMeT is not involved in JH biosynthesis (Burtenshaw et al, 2008;Zhang et al, 2010).…”

Section: Introductionmentioning

confidence: 46%

Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

Marchal

Zhang

Badisco

et al. 2011

Insect Biochemistry and Molecular Biology

106

105

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Section: Introductionmentioning

confidence: 46%

Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

Marchal

Zhang

Badisco

et al. 2011

Insect Biochemistry and Molecular Biology

106

105

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“…Since the FAMeT gene seems to be fairly conserved among insects, gene identification based only on its nucleotide or deduced amino acid sequence is difficult. A domains and motif analysis is therefore necessary, as suggested for the M. scutellaris FAMeT (Vieira et al, 2008). As reported for M. scutellaris FAMeT, the Ceratitis sequence shows three CK2 phosphorylation sites, suggesting a similar biosynthetic pathway for both genes.…”

Section: Discussionmentioning

confidence: 90%

“…: AM493718 and AM493719) described by Vieira et al (2008). As observed for the Ceratitis sequence, the isoform 1 shows two DM9 domains (GenBank accession no.…”

Section: Analysis Of Putative-famet Deduced Proteinmentioning

confidence: 82%

“…This supports the role of methyl transferase for the isolated gene in the medfly. Moreover, two DM9 domains are present in the FAMeT isoforms of M. scutellaris (Vieira et al, 2008) as well as in the putative-FAMeT of D. melanogaster (Burtenshaw et al, 2008). Both these genes also shared a CF domain at the N-terminal related to ''metal-binding'' proteins (Vieira et al, 2008), which is lacking in the medfly.…”

Section: Discussionmentioning

confidence: 97%

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The putative‐farnesoic acid O‐methyl transferase (FAMeT) gene of Ceratitis capitata: characterization and pre‐imaginal life expression

Vannini

Ciolfi

Spinsanti

et al. 2010

Arch Insect Biochem Physiol

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Farnesoic acid O-methyl transferase (FAMeT) is the enzyme involved in the penultimate step of insect juvenile hormone (JH) biosynthesis and is thus a key regulator in insect development and reproduction. We report the characterization of the putative-FAMeT in the medfly or Mediterranean fruit fly, Ceratitis capitata. This gene was identified by suppressive subtractive hybridization and completely sequenced by the screening of a medfly cDNA library. The obtained sequence was analyzed for conserved protein domain identification and its expression profile was evaluated by quantitative Real-Time PCR in medfly pre-imaginal life. The tissue expression of the isolated gene was verified by in situ hybridization on third instar larvae sections. The characterization of the isolated gene pointed out several typical features of methyl transferase genes. The pre-imaginal putative-FAMeT expression levels were consistent with JH titer change in Diptera. As recognized in some crustaceans, this gene seems to be widely expressed in the medfly as well. Ceratitis capitata is one of the most relevant agricultural pests against which insecticides and the sterile insect technique (SIT) are extensively used in spite of the well-known limitations of these approaches. Although results are not conclusive for the physiological role of the isolated gene, they suggest the characterization of a new gene in the Mediterranean fruit fly potentially involved in JH biosynthesis and may, therefore, have implications for pest control.

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“…Some reports identified specific functional genes involved in caste development (Corona et al, 1999;Yamazaki et al, 2006;Lattorff et al, 2007;Aamodt 2008;de Azevedo et al, 2008). Others screened clusters of genes that belonged to distinctly different evolutionary and functional groups and pathways (Evans et al, 1999;Patel et al, 2007;Vieira et al, 2008). In this study, differential feeding of female larvae promotes the occurrence of two different phenotypes from identical genotypes.…”

Section: Discussionmentioning

confidence: 78%

Screening of differentially expressed genes induced by water-soluble extracts from pollen during honeybee caste determination

Zhou¹,

Ye²,

Zhang³

et al. 2010

J. Anim. Feed Sci.

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In order to screen differentially expressed genes during caste differentiation induced by pollen, larvae of Apis mellifer ligustica were developed in an incubator under controlled temperature and relative humidity until emergence. Worker bee morphology was induced by addition of water-soluble extracts from pollen in larval diets. After all of the developmental periods, the external characters and developmental degree of ovaries were evaluated to confirm the caste of new adults. Transcripts from larvae of developing queens and workers were profiled by differential-display RT-PCR analysis and differentially expressed fragments were reconfirmed by dot-blot assay. Our results showed that caste differentiation was triggered by the intake of pollen extracts. Six transcript-derived fragments were isolated and three of them are reported for the first time. We conclude that differential display is a feasible approach to identifying differentially expressed genes. The identification of up-regulated caste-differentiation-related genes provided interesting clues about the activation of biochemical steps relevant to this progress.

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Farnesoic acid O‐methyl transferase (FAMeT) isoforms: Conserved traits and gene expression patterns related to caste differentiation in the stingless bee, Melipona scutellaris

Cited by 24 publications

References 26 publications

Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria

The putative‐farnesoic acid O‐methyl transferase (FAMeT) gene of Ceratitis capitata: characterization and pre‐imaginal life expression

Screening of differentially expressed genes induced by water-soluble extracts from pollen during honeybee caste determination

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