Farmers exposed to pesticides have almost five times more DNA damage: a meta-analysis study

Nascimento, Felipe de Araújo; Silva, Daniela de Melo e; Pedroso, Thays Millena Alves; Ramos, Jheneffer Sonara Aguiar; Parise, Michelle Rocha

doi:10.1007/s11356-021-15573-z

Cited by 11 publications

(13 citation statements)

References 74 publications

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“…Exposed individuals included in this study presented higher DNA damage when compared to non-exposed individuals (25.9 ± 6.0 and 20.0 ± 7.6, respectively; p <0.05). This finding is following studies from our research group that reported an association between DNA toxicity and pesticide exposure (Khayat et al 2013;Ramos et al 2020;Nascimento et al 2021). As presented in Figure 2, exposed individuals presented a negative correlation between % DNA and IFN-γ (p < 0.05) and % DNA and IL-6 (p < 0.05).…”

Section: Resultssupporting

confidence: 83%

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Effects of long-term direct exposure to pesticides towards immune function: a cross-sectional study

Souza

Nascimento

Ramos

et al. 2022

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Exposure to pesticides is an important risk factor for genotoxicity in farmworkers. Once there is a possibility that genotoxicity is related to the autoimmunity process, the present study aimed to characterize genotoxicity and possible immunological alterations related to chronic and direct exposure to a complex mixture of pesticides. It employed Interleukin–6 (IL-6), Interferon-gamma (IFN-γ), IL-12 and IL-10 as biomarkers of cellular immune function. DNA damage was greater in the exposed group (p < 0.05). Both IL-6 and IL-12 were significantly increased in the exposed group (p < 0.05 and < 0.01, respectively); on the other hand, Il-10 levels were decreased (p < 0.01). Evaluation of the association between cytokine levels and epidemiological variables showed a correlation between alcohol consumption and higher levels of IL-12 (p < 0.05). In addition, negative correlation between % DNA and IFN-γ (p < 0.05) as well as % DNA and IL-6 (p < 0.05) and positive correlation was found between IL-6 levels and absolute number of T CD8+ lymphocytes (p < 0.05) as well as between IL-10 levels and absolute number of T CD4+ lymphocytes (p < 0.05). No association between all the evaluation parameters was found in the non-exposed group. The observed alterations related to chronic and direct exposure to a complex mixture of pesticides may allow the monitoring of these workers to minimize the negative impact caused on their health.

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Section: Resultssupporting

confidence: 83%

“…In addition, it is important to note that pesticides present different mechanisms of action and the consequences of the exposure could differ according to the pattern of pesticide exposure (i.e. the type, amount, variety and mixtures) (Pedlowski et al 2012;Nascimento et al 2021).…”

Section: Resultsmentioning

confidence: 99%

Effects of long-term direct exposure to pesticides towards immune function: a cross-sectional study

Souza

Nascimento

Ramos

et al. 2022

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“…This indicates that TP53 mutations are related, at least to some extent, to carcinogen exposure. Still, it has been shown, in a population chronically exposed to pesticides, that there is accumulation of DNA lesions due to low DNA repair activity even at low doses of exposure (85). We found mutations in BRCA1/2 and PALB2 genes to be significantly associated with TMB in exposed patients.…”

Section: Discussionmentioning

confidence: 58%

“…The authors reported that, although possible confounding variables were not considered-such as age-and the difficulty to infer causality, there is evidence of an increase in genotoxic biomarkers in pesticide-exposed workers (84). A very recent meta-analysis highlights a significant impact of DNA damage for the pesticideexposed farmers, regardless of gender, age, pesticide type, or use of personal protective equipment (85). It is important to state that 1/3 of the products recently registered in Brazil contain active substances not approved, or even banned, by the European Commission and that the maximum residue level (i.e., pesticide concentration) considered acceptable in Brazil is higher than that allowed in the United States, Canada, European Union, and other BRICS countries (86).…”

Section: Discussionmentioning

confidence: 99%

Somatic DNA Damage Response and Homologous Repair Gene Alterations and Its Association With Tumor Variant Burden in Breast Cancer Patients With Occupational Exposure to Pesticides

et al. 2022

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Homologous recombination is a crucial pathway that is specialized in repairing double-strand breaks; thus, alterations in genes of this pathway may lead to loss of genomic stability and cell growth suppression. Pesticide exposure potentially increases cancer risk through several mechanisms, such as the genotoxicity caused by chronic exposure, leading to gene alteration. To analyze this hypothesis, we investigated if breast cancer patients exposed to pesticides present a different mutational pattern in genes related to homologous recombination (BRCA1, BRCA2, PALB2, and RAD51D) and damage-response (TP53) concerning unexposed patients. We performed multiplex PCR-based assays and next-generation sequencing (NGS) of all coding regions and flanking splicing sites of BRCA1, BRCA2, PALB2, TP53, and RAD51D in 158 unpaired tumor samples from breast cancer patients on MiSeq (Illumina) platform. We found that exposed patients had tumors with more pathogenic and likely pathogenic variants than unexposed patients (p = 0.017). In general, tumors that harbored a pathogenic or likely pathogenic variant had a higher mutational burden (p < 0.001). We also observed that breast cancer patients exposed to pesticides had a higher mutational burden when diagnosed before 50 years old (p = 0.00978) and/or when carrying BRCA1 (p = 0.0138), BRCA2 (p = 0.0366), and/or PALB2 (p = 0.00058) variants, a result not found in the unexposed group. Our results show that pesticide exposure impacts the tumor mutational landscape and could be associated with the carcinogenesis process, therapy response, and disease progression. Further studies should increase the observation period in exposed patients to better evaluate the impact of these findings.

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“…Due to the strong evidence about the association between pesticide exposure and DNA damage (Ali et al, 2018; Butinof et al, 2019; da Silva et al, 2008; Hilgert Jacobsen‐Pereira et al, 2018; Martínez‐Valenzuela et al, 2017; Nascimento et al, 2022; Perumalla Venkata et al, 2017; Shaham et al, 2001; Singh et al, 2011; Želježić & Garaj‐Vrhovac, 2001; Zúñiga et al, 2007), our goal was to determine if PON1 polymorphisms appear as susceptibility predictors for DNA damage in people who work and/or live in agricultural areas with intensive OP use.…”

Section: Introductionmentioning

confidence: 99%

DNAdamage in a Chilean population exposed to pesticides and its association withPON1(Q192RandL55M) susceptibility biomarker

Zúñiga-Venegas

Pancetti

2022

Environ and Mol Mutagen

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The active ingredients in pesticides are known to be genotoxic that can cause mutations, chromosomal aberrations, or other types of DNA damage. Early detection of genotoxicity reduces the risk of developing diseases such as cancer or suffering from reproductive disorders. In turn, the genotoxic risk depends on the intrinsic capability of the individual to metabolize and eliminate the xenobiotic from the organism. This study aimed to determine if two polymorphisms of paraoxonase-1 (PON1), which is involved in the metabolism of several organophosphate (OP) pesticides, are predictors of susceptibility to DNA damage in agricultural workers and inhabitants of rural areas chronically exposed to pesticides. A cross-sectional study was made considering three groups: agricultural workers (occupational exposure, OE, n = 85), rural inhabitants (environmental exposure, EE, n = 60), and an unexposed group conformed by people living far from agricultural areas (U, n = 33). The level of individual DNA damage was measured using the comet assay, and genotyping was done to determine the PON1 Q192R and L55M polymorphisms. Acetylcholinesterase and butyrilcholinesterase activities were also measured to determine exposure to OP. Individuals belonging to EE and OE groups displayed higher levels of DNA damage compared with U group (p < .001). OP exposure was the main predictor of genotoxicity (β = 16.19; 95% CI: 1.85, 30.52), instead of PON1 polymorphisms (β = À12.20; 95% CI: À27.87, 3.48). These results confirm the genotoxic effects of pesticide exposure and suggest that the catalytic efficiency of PON1 to metabolize OP pesticides becomes negligible in individuals with a history of long-term environmental or occupational exposure to these substances.

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Farmers exposed to pesticides have almost five times more DNA damage: a meta-analysis study

Cited by 11 publications

References 74 publications

Effects of long-term direct exposure to pesticides towards immune function: a cross-sectional study

Effects of long-term direct exposure to pesticides towards immune function: a cross-sectional study

Somatic DNA Damage Response and Homologous Repair Gene Alterations and Its Association With Tumor Variant Burden in Breast Cancer Patients With Occupational Exposure to Pesticides

DNAdamage in a Chilean population exposed to pesticides and its association withPON1(Q192RandL55M) susceptibility biomarker

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