Farm to table: colistin resistance hitchhiking through food

Talat, Absar; Miranda, Carla; Poeta, Patrícia; Khan, Asad

doi:10.1007/s00203-023-03476-1

Cited by 6 publications

(2 citation statements)

References 187 publications

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“…The increase in the usage of colistin accompanied by its uncontrolled use in agriculture globally have contributed to the dissemination of colistin-resistant Gram-negative pathogens worldwide [ 5 ]. Colistin resistance is mainly attributed to the decrease in the affinity of colistin to its target, either by the phosphoethanolamine modification of LPS by the two-component system, pmrAB , [ 6 ] or by the loss of LPS due to mutations in lipid biosynthesis genes [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Colistin Resistance in Acinetobacter baumannii and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon

Abou Fayad,

Haraoui,

Sleiman

et al. 2024

Microorganisms

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(1) Background: Infections with pan-drug-resistant (PDR) bacteria, such as A. baumannii, are becoming increasingly common, especially in healthcare facilities. In this study, we selected 15 colistin-resistant clinical A. baumannii isolates from a hospital in Beirut, Lebanon, to test combination therapies and determine their sequence types (STs) and the mechanism of colistin resistance using whole-genome sequencing (WGS). (2) Methods: Antimicrobial susceptibility testing via broth microdilution against 12 antimicrobials from different classes and growth rate assays were performed. A checkerboard assay was conducted on PDR isolates using six different antimicrobials, each in combination with colistin. Genomic DNA was extracted from all isolates and subjected to WGS. (3) Results: All isolates were resistant to all tested antimicrobials with the one exception that was susceptible to gentamicin. Combining colistin with either meropenem, ceftolozane–tazobactam, or teicoplanin showed synergistic activity. Sequencing data revealed that 67% of the isolates belonged to Pasteur ST2 and 33% to ST187. Furthermore, these isolates harbored a number of resistance genes, including blaOXA-23. Mutations in the pmrC gene were behind colistin resistance. (4) Conclusions: With the rise in antimicrobial resistance and the absence of novel antimicrobial production, alternative treatments must be found. The combination therapy results from this study suggest treatment options for PDR ST2 A. baumannii-infected patients.

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Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Colistin Resistance in Acinetobacter baumannii and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon

Abou Fayad,

Haraoui,

Sleiman

et al. 2024

Microorganisms

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“…This includes in animals where there has been appreciable use of colistin as a growth promoter in recent years [30], with studies now showing that 96% or more of total worldwide use of colistin is still in poultry and pig farming [31,32]. Concerns with the overuse of colistin including both animals and humans, and resultant resistance development via zoonotic gene transfers, coupled with its importance in treating resistant gramnegative infections to reduce morbidity and mortality, has resulted in the World Health Organization and others classifying colistin as an antibiotic of very high importance for use in humans with its use reserved [21,[33][34][35][36][37][38][39]. Alongside this, many countries now ban the use of colistin as a growth promoter in animal feeds and prophylactically to prevent bacterial infections [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future

Sarker,

Neeloy,

Habib

et al. 2024

Preprint

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Colistin is a last-resort antimicrobial for treating multidrug-resistant Gram-negative bacteria. Phe-notypic colistin resistance is highly associated with plasmid-mediated mobile colistin resistance (mcr) genes. mcr-bearing Enterobacteriaceae have been detected in many countries, with the emergence of colistin-resistant pathogens a global concern. This study assessed the distribution of mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 genes with the phenotypic colistin resistance in isolates from diarrheal infants and children in Bangladesh. Bacteria were identified using the API-20E biochemical panel and 16s rDNA gene sequencing. Polymerase chain reac-tions detected mcr gene variants in the isolates. Their susceptibilities to colistin were determined by agar dilu-tion and E-test by minimal inhibitory concentration (MIC) measurements. Over 30.0% (69/225) of isolates showed colistin resistance by agar dilution assessment (MIC> 2.0 μg/mL). Overall, 15.5% of isolates carried mcr genes (7, mcr-1; 17, mcr-2; 13, mcr-3; and co-occurrence occurred in 2 isolates). Clinical breakout MIC val-ues (≥ 4 μg/mL) were associated with 91.3% of mcr-positive isolates. The mcr-positive pathogens include twenty Escherichia spp., five Shigella flexneri, five Citrobacter spp., two Klebsiella pneumoniae, and three Pseudo-monas parafulva. mcr-genes appeared to be significantly associated with phenotypic colistin resistance phe-nomena (p=0.000), with 100% colistin-resistant isolates showing MDR phenomena. Age and sex of patients showed no significant association with detected mcr variants. Overall, mcr-associated colistin-resistant bacte-ria have emerged in Bangladesh, which warrants further research to determine their spread and instigate ac-tivities to reduce resistance.

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Association of Bacillus subtilis and Bacillus amyloliquefaciens: minimizes the adverse effects of necrotic enteritis in the gastrointestinal tract and improves zootechnical performance in broiler chickens

Rodrigues,

Silva,

Brugnera

et al. 2024

Poultry Science

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Farm to table: colistin resistance hitchhiking through food

Cited by 6 publications

References 187 publications

Molecular Characteristics of Colistin Resistance in Acinetobacter baumannii and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon

Molecular Characteristics of Colistin Resistance in Acinetobacter baumannii and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon

Mobile Colistin-Resistant Gene; mcr-1, mcr-2, mcr-3 Identified in Diarrheal Pathogens among Infants, Children, and Adults in Bangladesh: Implications for the Future

Association of Bacillus subtilis and Bacillus amyloliquefaciens: minimizes the adverse effects of necrotic enteritis in the gastrointestinal tract and improves zootechnical performance in broiler chickens

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