Farm and environmental distribution of Campylobacter and Salmonella in broiler flocks

Thakur, Siddhartha; Brake, J.; Keelara, Shivaramu; Zou, Ming; Susick, E.

doi:10.1016/j.rvsc.2012.07.014

Cited by 44 publications

(35 citation statements)

References 49 publications

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“…Antimicrobial-resistant Salmonella could re-circulate in the farms because of litter contamination during different production cycles. Therefore, litter in broiler farms may be an important reservoir of Salmonella , consistent with a speculation in a report from the USA [62]. In summary, we concluded that serious Salmonella contamination occurs in farms during production cycles, as does cross-contamination among farms owned by the same company.…”

Section: Discussionsupporting

confidence: 89%

Distribution and dissemination of antimicrobial-resistant Salmonella in broiler farms with or without enrofloxacin use

Shang¹,

Wei²,

Kang³

2018

BMC Vet Res

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BackgroundSalmonella is a major zoonotic food-borne pathogen that persists on poultry farms, and animals undergo reinfection with endemic strains. The present study aimed to investigate the characteristics and dissemination of antimicrobial-resistant Salmonella within and between broiler farms that used enrofloxacin and those that did not.ResultsCloacal and environmental (litter, feed, and water) samples from two selected flocks in each of 12 farms owned by the same company were collected three times over a 30-day period of two production cycles during 2015–2016. The rate of Salmonella isolation was 7.8% (123/1584). Nine Salmonella serotypes (116 isolates) and seven untypable isolates were identified, and Salmonella Montevideo was the most prevalent serotype. Azithromycin-resistant (17.9%) and colistin-resistant (3.3%) isolates were detected, and multidrug-resistant isolates (43.1%) were also observed. No isolate was resistant to enrofloxacin or ciprofloxacin; however, intermediate resistance to enrofloxacin was significantly higher (P < 0.05) in farms that used enrofloxacin than in those that did not. The rate of multi-drug resistance among litter isolates (25/44, 56.8%) was significantly higher (P < 0.05) than that among cloacal swab (24/67, 35.8%) and feed (4/12, 33.3%) isolates. Pulsed-field gel electrophoresis (PFGE) analysis of strains of the same serotype was conducted to determine their epidemiological relationship. The PFGE types were classified into 31 groups with a 100% correlation cutoff in dendrograms for Salmonella Montevideo isolates, which showed 100% genomic identity based on age, sample type, flock, and production cycle within and between farms.ConclusionThe present study highlights the occurrence of horizontal transmission and cyclic contamination with antimicrobial-resistant Salmonella in broiler farms owned by the same company. Litter may be a good indicator of indoor environmental contamination with antimicrobial-resistant Salmonella on farms. Additionally, enrofloxacin use may be one of the factors promoting resistance towards it in Salmonella.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1590-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Distribution and dissemination of antimicrobial-resistant Salmonella in broiler farms with or without enrofloxacin use

Shang¹,

Wei²,

Kang³

2018

BMC Vet Res

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“…The serotypes Enteritidis and Typhimurium have been reported in other research studies (Abd-Elghany et al, 2015;Suresh et al, 2011;Thakur et al, 2013) as the most common in chickens. This study, however, did not detect any isolates of these serotypes in chicken product or fecal samples.…”

Section: Resultsmentioning

confidence: 52%

DNA Profiles of Salmonella Spp. Isolated from Chicken Products and From Broiler and Human Feces

Tejada

Silva

Lopes

et al. 2016

Rev. Bras. Cienc. Avic.

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Salmonella species have been isolated from various kinds of food and are accountable for outbreaks of foodborne diseases in humans. This study aimed at identifying the similarities between the DNA profiles of Salmonella isolated from chicken feces, chicken products, and human feces in southern Brazil. Six hundred samples were collected (200 from chicken products, 200 from broiler chicken feces, and 200 from human feces) and tested for the presence of Salmonella. Isolates proven to be Salmonella compatible by biochemical and serological tests were tested by the Polymerase Chain Reaction. Their DNA profiles were then analyzed by PFGE and rep-PCR. Salmonella was isolated from 16 out of 600 analyzed samples, with Schwarzengrund serotype presenting the highest incidence, followed by Mbandaka in chicken meat and fecal samples, and Panama in human fecal samples. Some strains isolated from chicken fecal and product samples were indistinguishable by the molecular methods used in the study, suggesting that that the contamination of the broilers on the farm can be transmitted the processed products.

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“…; Thakur et al . ). Although these various results cannot be compared due to differences in sampling and isolation protocols, the prevalence of the two pathogens remains high in both Japanese and foreign broiler farms.…”

Section: Discussionmentioning

confidence: 97%

Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: results of a 2-year distribution and circulation dynamics audit

et al. 2016

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Farm and environmental distribution of Campylobacter and Salmonella in broiler flocks

Cited by 44 publications

References 49 publications

Distribution and dissemination of antimicrobial-resistant Salmonella in broiler farms with or without enrofloxacin use

Distribution and dissemination of antimicrobial-resistant Salmonella in broiler farms with or without enrofloxacin use

DNA Profiles of Salmonella Spp. Isolated from Chicken Products and From Broiler and Human Feces

Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: results of a 2-year distribution and circulation dynamics audit

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