Far-red light inhibits germination through DELLA-dependent stimulation of ABA synthesis and ABI3 activity

Piskurewicz, Urszula; Turečková, Veronika; Lacombe, Éric; Lopez‐Molina, Luis

doi:10.1038/emboj.2009.170

Cited by 129 publications

(153 citation statements)

References 40 publications

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“…1 G and I, and 2 A and B). RGA enhances RGL2 function and they are both known to stimulate ABA biosynthesis (50), but it remains to be established whether this is through a direct effect on ABA1 expression. We propose that the low germination rates of spt mutant Ler seeds (Fig.…”

Section: Resultsmentioning

confidence: 99%

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

Vaistij

Gan

Penfield

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

115

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Freshly matured seeds exhibit primary dormancy, which prevents germination until environmental conditions are favorable. The establishment of dormancy occurs during seed development and involves both genetic and environmental factors that impact on the ratio of two antagonistic phytohormones: abscisic acid (ABA), which promotes dormancy, and gibberellic acid, which promotes germination. Although our understanding of dormancy breakage in mature seeds is well advanced, relatively little is known about the mechanisms involved in establishing dormancy during seed maturation. We previously showed that the SPATULA (SPT) transcription factor plays a key role in regulating seed germination. Here we investigate its role during seed development and find that, surprisingly, it has opposite roles in setting dormancy in Landsberg erecta and Columbia Arabidopsis ecotypes. We also find that SPT regulates expression of five transcription factor encoding genes: ABA-INSENSITIVE4 (ABI4) and ABI5, which mediate ABA signaling; REPRESSOR-OF-GA (RGA) and RGA-LIKE3 involved in gibberellic acid signaling; and MOTHER-OF-FT-AND-TFL1 (MFT ) that we show here promotes Arabidopsis seed dormancy. Although ABI4, RGA, and MFT are repressed by SPT, ABI5 and RGL3 are induced. Furthermore, we show that RGA, MFT, and ABI5 are direct targets of SPT in vivo. We present a model in which SPT drives two antagonistic "dormancy-repressing" and "dormancy-promoting" routes that operate simultaneously in freshly matured seeds. Each of these routes has different impacts and this in turn explains the opposite effect of SPT on seed dormancy of the two ecotypes analyzed here.phytohormone analyses | chromatin immunoprecipitation | transcriptomic analyses

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Section: Resultsmentioning

confidence: 99%

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

Vaistij

Gan

Penfield

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

115

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“…The ABA insensitivity and tolerance to paclobutrazol of ZFP3ox lines could be influenced by reduced transcription of the DELLA factor RGL2 in ZFP3ox seedlings, which is a negative regulator of GA responses (Lee et al, 2002;Tyler et al, 2004). RGL2 was shown to control germination by enhancing ABA-dependent ABI3 and ABI5 activities, which act as final repressors of germination (Piskurewicz et al, 2008(Piskurewicz et al, , 2009. Although the transcript levels of ABI5 and RGL2 were lower in ZFP3ox seedlings, they are probably not direct targets of ZFP3, as their expression was not altered by ZFP3 overexpression after short-term ABA induction (Fig.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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Seed germination is controlled by environmental signals, including light and endogenous phytohormones. Abscisic acid (ABA) inhibits, whereas gibberellin promotes, germination and early seedling development, respectively. Here, we report that ZFP3, a nuclear C2H2 zinc finger protein, acts as a negative regulator of ABA suppression of seed germination in Arabidopsis (Arabidopsis thaliana). Accordingly, regulated overexpression of ZFP3 and the closely related ZFP1, ZFP4, ZFP6, and ZFP7 zinc finger factors confers ABA insensitivity to seed germination, while the zfp3 zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of several ABA-induced genes, such as RESPONSIVE TO ABSCISIC ACID18 and transcription factor ABSCISIC ACID-INSENSITIVE4 (ABI4), in ZFP3 overexpression seedlings suggests that ZFP3 negatively regulates ABA signaling. Analysis of ZFP3 overexpression plants revealed multiple phenotypic alterations, such as semidwarf growth habit, defects in fertility, and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants indicates that ZFP3 enhances red light signaling by photoreceptors other than phytochrome A and additively increases ABA insensitivity conferred by the abi2, abi4, and abi5 mutations. These data support the conclusion that ZFP3 and the related ZFP subfamily of zinc finger factors regulate light and ABA responses during germination and early seedling development.

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“…Mutants deficient in ABA synthesis or signaling in general show reduced dormancy, often accompanied by defects in the seed maturation program, such as reduced reserve accumulation and desiccation tolerance (Nambara et al, 1994). In seeds, the action of ABA is antagonized by that of gibberellin (GA), and numerous studies have shown that an intricate web of crossregulation between ABA and GA levels lies at the heart of the control of seed germination (Seo et al, 2006;Piskurewicz et al, 2008Piskurewicz et al, , 2009. Environmental signals that influence dormancy or germination have been shown to result in the transcriptional regulation of GA and ABA metabolism in the imbibed seed.…”

Section: Introductionmentioning

confidence: 99%

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

et al. 2011

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Summer annuals overwinter as seeds in the soil seed bank. This is facilitated by a cold-induced increase in dormancy during seed maturation followed by a switch to a state during seed imbibition in which cold instead promotes germination. Here, we show that the seed maturation transcriptome in Arabidopsis thaliana is highly temperature sensitive and reveal that low temperature during seed maturation induces several genes associated with dormancy, including DELAY OF GERMINATION1 (DOG1), and influences gibberellin and abscisic acid levels in mature seeds. Mutants lacking DOG1, or with altered gibberellin or abscisic acid synthesis or signaling, in turn show reduced ability to enter the deeply dormant states in response to low seed maturation temperatures. In addition, we find that DOG1 promotes gibberellin catabolism during maturation. We show that C-REPEAT BINDING FACTORS (CBFs) are necessary for regulation of dormancy and of GA2OX6 and DOG1 expression caused by low temperatures. However, the temperature sensitivity of CBF transcription is markedly reduced in seeds and is absent in imbibed seeds. Our data demonstrate that inhibition of CBF expression is likely a critical feature allowing cold to promote rather than inhibit germination and support a model in which CBFs act in parallel to a low-temperature signaling pathway in the regulation of dormancy.

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Far-red light inhibits germination through DELLA-dependent stimulation of ABA synthesis and ABI3 activity

Cited by 129 publications

References 40 publications

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

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