Far-infrared radiation stimulates platelet-derived growth factor mediated skeletal muscle cell migration through extracellular matrix-integrin signaling

Abstract

Despite increased evidence of bio-activity following far-infrared (FIR) radiation, susceptibility of cell signaling to FIR radiation-induced homeostasis is poorly understood. To observe the effects of FIR radiation, FIR-radiated materials-coated fabric was put on experimental rats or applied to L6 cells, and microarray analysis, quantitative real-time polymerase chain reaction, and wound healing assays were performed. Microarray analysis revealed that messenger RNA expressions of rat muscle were stimulated by FIR radiation in a dose-dependent manner in amount of 10% and 30% materials-coated. In 30% group, 1,473 differentially expressed genes were identified (fold change [FC] > 1.5), and 218 genes were significantly regulated (FC > 1.5 and p < 0.05). Microarray analysis showed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and cell migration-related pathways were significantly stimulated in rat muscle. ECM and platelet-derived growth factor (PDGF)-mediated cell migration-related genes were increased. And, results showed that the relative gene expression of actin beta was increased. FIR radiation also stimulated actin subunit and actin-related genes. We observed that wound healing was certainly promoted by FIR radiation over 48 h in L6 cells. Therefore, we suggest that FIR radiation can penetrate the body and stimulate PDGF-mediated cell migration through ECM-integrin signaling in rats.

Far-infrared rays enhance mitochondrial biogenesis andGLUT3expression under low glucose conditions in rat skeletal muscle cells

“…Microarray data extracted from a previous study [ 23 ] were assembled for GO map analysis. We confirmed that pathways related to glucose transport and regulation were stimulated by FIR in rat skeletal muscle ( Fig.…”

“…In our previous study, microarray analysis revealed that mRNA expression in rat muscle cells was stimulated by FIR radiation in a dose-dependent manner at 10% and 30% BMCF [ 23 ]. Additional analysis of genetic data through GO analysis confirmed the possibility that FIR could affect glucose metabolism ( Fig.…”

“…The cells were used between passage 29 and 33. For FIR radiation conditions, 0% and 30% BMCF was attached inside the culture dish at a distance of 1 cm from the cells [ 23 ], followed by incubation at 37°C in 5% CO 2 for 24 and 72 h. The experimental design is shown in Supplementary Fig. 1 .…”

Effects of Far-Infrared Therapy on Foot Circulation Among Hemodialysis Patients With Diabetes Mellitus

“…FIR therapy also enhances HO-1 synthesis, activates eNOS, inhibits vessel endothelium growth, reduces oxidative vessel sclerosis, and improves vessel repair effects (Chang, 2018;Choi et al, 2016;Lin et al, 2016). Chang (2018) and Lee et al (2019) also mentioned that FIR treatment can reduce and delay IL-6 and TNF-a responses, which may improve vascular endothelial cell function and help reduce the inflammatory response. In the present study, we found that DPA pulse strength; DPA blood flow strength; soreness, tingling, and coldness in the foot; right foot pain; and sensitivity to tactility and pressure improved significantly in the experimental group after 6 months of FIR therapy, suggesting that the intervention may have eased the acute-phase inflammatory response or promoted healing of peripheral arterial occlusion.…”

“…Indeed, research has shown that FIR therapy reduces atherosclerosis in humans (Chen, Lee, et al, 2016;Chen, Yang, et al, 2016;Choi et al, 2016;Lo et al, 2019;Yu et al, 2006). Due to FIR's ability to increase vasodilation, improve vascular endothelial cell function, slow the inflammatory response, and reduce endothelial oxidative stress in addition to evidence that it stimulates skeletal muscle cell migration mediated by platelet-derived growth factor and tissue healing (Lee et al, 2019), clinicians have used such treatment widely to treat hemodialysis fistulas (Lee et al, 2019).…”