FaMYB44.2, a transcriptional repressor, negatively regulates sucrose accumulation in strawberry receptacles through interplay with FaMYB10

Wei, Lingzhi; Mao, Wendong; Jia, Meiru; Xing, Sinian; Ali, Usman; Zhao, Yaoyao; Chen, Yating; Cao, Minglin; Dai, Zhengrong; Zhang, Kai; Dou, Zhechao; Jia, Wensuo; Li, Bingbing

doi:10.1093/jxb/ery249

Cited by 82 publications

(52 citation statements)

References 73 publications

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“…8f). Beside of the above morphological phenotypes, we also assessed the transcriptional level of downstream genes which indicated fruit ripening, including the softeningrelated genes polygalacturonase (PG), and beta-xylosidase1 (XYL1); the anthocyanin biosynthesis genes dihydroflavonol 4-reductase (DFR) and UDPglucose flavonoid 3-O-glycosyltransferase (UFGT); and the sugar-related genes hexokinase (HXK2) [68][69][70][71]. QRT-PCR analysis showed that the transcript levels of PG, XYL1, DFR, UFGT, and HXK2 were significantly downregulated in the FvPME-RNAi fruits, but upregulated in the overexpressor fruits, compared with the control (Fig.…”

Section: Fvpme38 and Fvpme39 Are Involved In Regulation Of Fruit Softmentioning

confidence: 99%

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

et al. 2020

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Background: Pectin methylesterase (PME) is a hydrolytic enzyme that catalyzes the demethylesterification of homogalacturonans and controls pectin reconstruction, being essential in regulation of cell wall modification. During fruit ripening stage, PME-mediated cell wall remodeling is an important process to determine fruit firmness and softening. Strawberry fruit is a soft fruit with a short postharvest life, due to a rapid loss of firm texture. Hence, preharvest improvement of strawberry fruit rigidity is a prerequisite for extension of fruit refreshing time. Although PME has been well characterized in model plants, knowledge regarding the functionality and evolutionary property of PME gene family in strawberry remain limited. Results: A total of 54 PME genes (FvPMEs) were identified in woodland strawberry (Fragaria vesca 'Hawaii 4'). Phylogeny and gene structure analysis divided these FvPME genes into four groups (Group 1-4). Duplicate events analysis suggested that tandem and dispersed duplications effectively contributed to the expansion of the PME family in strawberry. Through transcriptome analysis, we identified FvPME38 and FvPME39 as the most abundantexpressed PMEs at fruit ripening stages, and they were positively regulated by abscisic acid. Genetic manipulation of FvPME38 and FvPME39 by overexpression and RNAi-silencing significantly influences the fruit firmness, pectin content and cell wall structure, indicating a requirement of PME for strawberry fruit softening. Conclusion: Our study globally analyzed strawberry pectin methylesterases by the approaches of phylogenetics, evolutionary prediction and genetic analysis. We verified the essential role of FvPME38 and FvPME39 in regulation of strawberry fruit softening process, which provided a guide for improving strawberry fruit firmness by modifying PME level.

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Section: Fvpme38 and Fvpme39 Are Involved In Regulation Of Fruit Softmentioning

confidence: 99%

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

et al. 2020

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“…The transcription factor AtMYB44 is involved in embryogenesis, seed maturation, responses to pathogens, insects and environmental stresses in Arabidopsis and functions in association with the ethylene signalling pathway (Liu et al ., ). In strawberry, FaMYB44.2 negatively regulates the accumulation of soluble sugar and malic acid content, and may be involved in regulating the production of aroma compounds (Wei et al ., ). MdMYB44 has also been shown to act as a negative regulator in response to cold and salt stress in apple calli (Wu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Genetic control of α‐farnesene production in apple fruit and its role in fungal pathogenesis

et al. 2019

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Terpenes are important compounds in plant trophic interactions. A meta-analysis of GC-MS data from a diverse range of apple (Malus 3 domestica) genotypes revealed that apple fruit produces a range of terpene volatiles, with the predominant terpene being the acyclic branched sesquiterpene (E,E)-a-farnesene. Four quantitative trait loci (QTLs) for a-farnesene production in ripe fruit were identified in a segregating 'Royal Gala' (RG) 3 'Granny Smith' (GS) population with one major QTL on linkage group 10 co-locating with the MdAFS1 (a-farnesene synthase-1) gene. Three of the four QTLs were derived from the GS parent, which was consistent with GC-MS analysis of headspace and solvent-extracted terpenes showing that cold-treated GS apples produced higher levels of (E,E)-a-farnesene than RG. Transgenic RG fruit downregulated for MdAFS1 expression produced significantly lower levels of (E,E)-a-farnesene. To evaluate the role of (E,E)-a-farnesene in fungal pathogenesis, MdAFS1 RNA interference transgenic fruit and RG controls were inoculated with three important apple post-harvest pathogens [Colletotrichum acutatum, Penicillium expansum and Neofabraea alba (synonym Phlyctema vagabunda)]. From results obtained over four seasons, we demonstrate that reduced (E,E)-a-farnesene is associated with decreased disease initiation rates of all three pathogens. In each case, the infection rate was significantly reduced 7 days post-inoculation, although the size of successful lesions was comparable with infections on control fruit. These results indicate that (E,E)-a-farnesene production is likely to be an important factor involved in fungal pathogenesis in apple fruit.

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“…Transcriptional regulation of CHS was achieved by a complex of R2R3-MYB and basic helix-loop-helix (bHLH) transcription factors (TFs) [49]. In strawberry, FaMYB10 regulates FaCHS [40,50]. Considering this regulatory role, we evaluated an effector-reporter system with FaMYB10 as the effector and GUS driven by FaCHS promoter as the reporter to study cellular signal transduction in strawberry fruits.…”

Section: Discussionmentioning

confidence: 99%

“…Besides protoplast-based transient gene expression, transient expression has been increasingly reported in the fruits of many plant species, among which strawberry may be the one that was most extensively studied [6,21,[39][40][41]. We previously conducted a comprehensive study on transient gene expression to optimize and standardize this technique in strawberry fruits [39].…”

Section: Introductionmentioning

confidence: 99%

An effector-reporter system to study cellular cellular signal transduction in strawberry fruit (Fragaria ananassa)

Zeng

Wang

et al. 2020

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Background: An effector-reporter system is a powerful tool used to study cellular signal transduction; however, this technique has been traditionally adopted in protoplasts. A similar system to study cellular signal transduction in fruits has not yet been established. In this paper, we aim to establish an effector-reporter system for strawberry fruit, a model non-climacteric fruit.Results: We compared four different reporters, including GUS, GFP, FLuc, and RLuc, as well as combination of two pairs of reporters, including GUS/GFP and RLuc/FLuc, by statistically analyzing the pattern of reporter activities exhibited among individual fruits. Among the reporters examined, GUS was the best choice. Further sampling indicated that a minimum of five mixed specimens with five fruits each is necessary for an assay. With the so-established system, FaCHS promoter demonstrated a sensitive response to FaMYB10 effector as well as to the external and internal cues implicated in the regulation of fruit ripening.Conclusion: A step-by step protocol was established to study cellular signal transduction in strawberry fruits. This finding will contribute to the molecular investigation of fruit ripening.

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FaMYB44.2, a transcriptional repressor, negatively regulates sucrose accumulation in strawberry receptacles through interplay with FaMYB10

Abstract: FaMYB44.2 is a novel transcriptional repressor that modulates both ripening-related and jasmonic acid-related sucrose accumulation in strawberry receptacles.

Cited by 82 publications

References 73 publications

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

Genetic control of α‐farnesene production in apple fruit and its role in fungal pathogenesis

An effector-reporter system to study cellular cellular signal transduction in strawberry fruit (Fragaria ananassa)

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FaMYB44.2, a transcriptional repressor, negatively regulates sucrose accumulation in strawberry receptacles through interplay with FaMYB10

Abstract: FaMYB44.2 is a novel transcriptional repressor that modulates both ripening-related and jasmonic acid-related sucrose accumulation in strawberry receptacles.

Cited by 82 publications

References 73 publications

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

Genetic control of α‐farnesene production in apple fruit and its role in fungal pathogenesis

An effector-reporter system to study cellular cellular signal transduction in strawberry fruit (Fragaria ananassa)﻿

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An effector-reporter system to study cellular cellular signal transduction in strawberry fruit (Fragaria ananassa)