Familial OKT4 epitope deficiency: Studies on antigen density and lymphocyte function

Aozasa, Mieko; Amino, Nobuyuki; Iwatani, Yoshinori; Tamaki, Haruo; Watanabe, Yukihiko; Sato, Haruhiko; Hochito, Kazunori; Umeda, Keiko; Gunji, T.; Yoshizawa, Toshiyuki; Iyama, Shigeru; Miyai, Kiyoshi

doi:10.1016/0090-1229(85)90134-5

Cited by 15 publications

(8 citation statements)

References 7 publications

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“…The biologic significance of this observation is not clear. No gross abnormalities in the function of PBMC from T4 epitopedeficient donors have been identified in assessments of proliferation and IgG production in response to various mitogens (6,14,15). Such systems, however, may be too insensitive to detect more subtle immunologic alterations.…”

Section: Discussionmentioning

confidence: 97%

Correlation between systemic lupus erythematosus and T4 epitope phenotype

Stohl

Singer

1987

Arthritis & Rheumatism

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Three groups of black subjects (systemic lupus erythematosus patients, patients with nonrheumatic disease, and normal subjects) were screened for the expression of the T4 epitope, as recognized by the monoclonal antibody OKT4. We found that the T cell subsets within each group were similar, regardless of the T4 epitope phenotype (intact, intermediate, or deficient). In the subgroup of Jamaican subjects, there was an association between systemic lupus erythematosus and the T4 epitope-intermediate and T4 epitope-deficient phenotypes; this association was not detected in the nonJamaican population.The CD4 (T4) surface molecule is a T cell differentiation antigen expressed on T cells that exhibit helperhnducer function (1). Surface CD4 expresses many different determinants (epitopes) that can be individually recognized by different anti-CD4 monoclonal antibodies (MAb). A heterogeneity in expression of the epitope recognized by the MAb OKT4 (T4 epitope) has been described (2-6), and has given rise to 3 T4 epitope phenotypes (as detected by flow cytometry): T4 epitope-intact, T4 epitope-deficient, and T4 epitope-intermediate (3). This polymorphism is inherited in an autosomal codominant manner (3,4), 36527.with the T4 epitope-intermediate phenotype representing the heterozygous state. The T4 epitope-deficient and T4 epitope-intermediate phenotypes have been described in black subjects (2-6), but not in white subjects.We have previously described 3 black families whose members manifested systemic lupus erythematosus (SLE) in association with the T4 epitope-deficient phenotype (7). It is not known whether a correlation exists between T4 epitope phenotype and SLE in the general black population, or even within certain limited subpopulations. To address this question, the T4 epitope phenotype was determined in 3 groups of black subjects: those with SLE, those with nonrheumatic disorders, and those without apparent disease (normal subjects). We found that the T4 epitopedeficient and T4 epitopeintermediate phenotypes are more prevalent in black Jamaicans with SLE than in either black Jamaican control group. In nowJamaican black subjects, no such correlation between SLE and T4 epitope phenotype could be demonstrated.

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Section: Discussionmentioning

confidence: 97%

Correlation between systemic lupus erythematosus and T4 epitope phenotype

Stohl

Singer

1987

Arthritis & Rheumatism

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“…The percentages of positively staining cells in some intermediate variants were reportedly lower when stained with 0KT4 than with Leu-3a MoAb [4,8,14]. Aozasa et al [13] reported 0 43% of a Japanese study group to be OKT4 epitope-deficient. In 1984 Joffe & Rabson [5] using fluorescent microscopy reported 4 3''/o OKT4 epitope deficiency in 70 black South African children with measles.…”

Section: Introductionmentioning

confidence: 99%

“…Previously in 1984 an incidence of 43% of 70 black South African children with measles had been reported to have OKT4 epitojw deficiency using fluorescent microscopy [5]. Of African Americans 8-2% [4] and 7-4% [17] and of Japanese 043% [13] have been reported to be OKT4 epitope-deficient.…”

Section: Discissionmentioning

confidence: 99%

“…Cases of OKT4 epitope deficieney have previously been reported in Comoran Senegalese [1], Japanese [3,7,13], African American [4,6,8,14], Jamaican [15]. Brazilian [16] and black South African [5] populations.…”

Section: Introductionmentioning

confidence: 99%

“…Two cases in white subjects have been reported to carry this phenotype [12.17]. Family studies have suggested that the inheritance of the OKT4 epitope-deficient phenotype may be autosomal codominant [3,4,8,13,17], autosomal dominant [7] or autosomal recessive [6]. Stohl & Kunkel [8] and Aozaso et al [13] concluded that the inheritance was autosomal co-dominant based on family studies in which a decreased number of 0KT4 epitopes (epitope intermediate phenotyF)e) was manifested by a decrease in peak position of fluorescent intensity.…”

Section: Introductionmentioning

confidence: 99%

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Flow cytometry analysis of OKT4 epitope deficiency in South African black children

Hughes¹,

Goddard²,

Bouic

et al. 1994

Clinical and Experimental Immunology

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SUMMARYA case of OKT4 epitope deficiency referred for investigation with suspected immunodeficiency is described. Flow cytometry analysis of OKT4 epitope deficiency in a study group of healthy black children showed different manifestations of the lack of OKT4 epitope; a complete lack of OKT4*CD4^ peripheral blood lymphocytes (PBL) with normal numbers of OKT4A* and Leu3a+CD4^ PBL, decreased percentage OKT4*CD4^ compared with OKT4A'^ and Leu-3a^CD4* PBL, decreased fiuorescent staining intensity with OKT4 and a biphasic OKT4 staining pattern associated with a reduced OKT4/Leu-3a ratio. The percentage and fluorescent intensity of OKT4+CD4^ PBL in the study group were significantly lower (P < 0 0001) than Leu-3a^CD4+ and OKT4A*CD4^ PBL. There is thus considerable risk of under-estimating the number of CD4* cells in black South Africans if the OKT4 MoAb is used.

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2021

Flow Cytometry of Hematological Malignancies

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Familial OKT4 epitope deficiency: Studies on antigen density and lymphocyte function

Cited by 15 publications

References 7 publications

Correlation between systemic lupus erythematosus and T4 epitope phenotype

Correlation between systemic lupus erythematosus and T4 epitope phenotype

Flow cytometry analysis of OKT4 epitope deficiency in South African black children

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