Faltung einer RNA‐Haarnadel in der dicht gedrängten Zelle

Folding of ribonucleic acids (RNAs) is driven by several factors, such as base pairing and stacking, chain entropy, and ion‐mediated electrostatics, which have been studied in great detail. However, the power of background molecules in the cellular milieu is often neglected. Herein, we study the effect of common osmolytes on the folding equilibrium of a hairpin‐structured RNA and, using pressure perturbation, provide novel thermodynamic and volumetric insights into the modulation mechanism. The presence of TMAO causes an increased thermal stability and a more positive volume change for the helix‐to‐coil transition, whereas urea destabilizes the hairpin and leads to an increased expansibility of the unfolded state. Further, we find a strong interplay between water, salt, and osmolyte in driving the thermodynamics and defining the temperature and pressure stability limit of the RNA. Our results support a universal working mechanism of TMAO and urea to (de)stabilize proteins and the RNA.

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Modulation of the Thermodynamic Signatures of an RNA Thermometer by Osmolytes and Salts

Gao

Arns

Winter

2017

Angewandte Chemie

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Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells

Chen

Dai

et al. 2018

Angewandte Chemie

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Because of the absence of methods for tracking RNA G-quadruplex dynamics,e specially the folding and unfolding of this attractive structure in live cells,u nderstanding of the biological roles of RNAG -quadruplexes is so far limited. Herein, we report an ew red-emitting fluorescent probe, QUMA-1,f or the selective,c ontinuous,a nd real-time visualization of RNAG-quadruplexes in live cells.The applications of QUMA-1 in several previously intractable applications, including live-cell imaging of the dynamic folding,u nfolding, and movement of RNAG-quadruplexes and the visualization of the unwinding of RNAG -quadruplexes by RNAh elicase have been demonstrated. Notably,o ur real-time results revealed the complexity of the dynamics of RNAG -quadruplexes in live cells.W eanticipate that the further application of QUMA-1 in combination with appropriate biological and imaging methods to explore the dynamics of RNAGquadruplexesw ill uncover more information about the biological roles of RNAG-quadruplexes.

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Compaction of RNA Duplexes in the Cell**

et al. 2020

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The structure and flexibility of RNA depend sensitively on the microenvironment. Using pulsed electron-electron double-resonance (PELDOR) spectroscopy combined with advanced labeling techniques, we show that the structure of double-stranded RNA (dsRNA) changes upon internalization into Xenopus laevis oocytes. Compared to dilute solution, the dsRNA A-helix is more compact in cells. We recapitulate this compaction in a densely crowded protein solution. Atomic-resolution molecular dynamics simulations of dsRNA capture semi-quantitatively the compaction, and identify non-specific electrostatic interactions between proteins and dsRNA as a possible driver of this effect.

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Faltung einer RNA‐Haarnadel in der dicht gedrängten Zelle

Abstract: + + ]D iese Autoren haben zu gleichen Teilen zu der Arbeit beigetragen.Hintergrundinformationen und ORCIDs der Autoren zu diesem Beitrag sind im WWW unter http://dx.

Cited by 10 publications

References 48 publications

Modulation of the Thermodynamic Signatures of an RNA Thermometer by Osmolytes and Salts

Modulation of the Thermodynamic Signatures of an RNA Thermometer by Osmolytes and Salts

Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells

Compaction of RNA Duplexes in the Cell**

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