False positives from impurities result in incorrect functional characterization of receptors in chemosensory studies

Schorkopf, Dirk Louis P.; Molnár, Béla Péter; Solum, Marit; Larsson, Mattias C.; Millar, Jocelyn G.; Kárpáti, Zsolt; Dekker, Teun

doi:10.1016/j.pneurobio.2019.101661

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…The use of triple electroantennography reduces the risk of uneven sampling of the olfactory receptor 23,24 . Artefactual responses to impurities have been reported 45,46 , but were refuted here by the GC-EAD analysis of the same compounds.…”

Section: Discussionmentioning

confidence: 74%

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Olfaction in Tephritidae: a balance between detection and discrimination

Ramiaranjatovo,

Charlery de la Masselière,

Dekker

et al. 2024

Preprint

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Phytophagous insects are capable of detecting and locating suitable hosts, which emit volatile compounds. Polyphagous species appear to have a complex olfactory strategy given that their numerous hosts have diverse emission profiles. In particular, their hosts' volatile emissions share some of the same compounds, providing chemical bridges between them. However, the behavioural plasticity observed in insect host selection suggests that other volatiles have a complementary role. Here we explore how polyphagous insect specialization to detect and discriminate between hosts has driven their chemical selectivity. We predicted in silico a functional trade-off between these two functions, using a neuronal model of an olfactory system. The volatile emissions of intact or mechanically damaged fruit from 28 different species were fed into the model and analysed using gas chromatography coupled with mass spectrometry. Using triple point electroantennography and a behavioural assay, we studied the olfactory response of Tephritidae fruit fly species that oviposit on fruit. We observed experimentally two predictions of our model for host discriminating olfactory systems: the olfactory responses of eight species were negatively correlated with the compound's degree of sharedness among fruit emissions; female Bactrocera dorsalis showed a behavioural preference for non-shared over shared fruit compounds. A dose-dependent switch in the fly's preference confirmed the ecological importance of shared fruit compounds. Thus, we propose that insect olfactory systems are chemically tuned to detect suitable hosts and accurately discriminate between them.

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Section: Discussionmentioning

confidence: 74%

“…Artefactual responses to impurities have been reported 45,46 , but were refuted here by the GC-EAD analysis of the same compounds.…”

Section: Discussionmentioning

confidence: 76%

Olfaction in Tephritidae: a balance between detection and discrimination

Ramiaranjatovo,

Charlery de la Masselière,

Dekker

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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“…Another very important (but often neglected) confounding factor in odor sensation studies is the presence of impurities in the sample, which is exacerbated at very high stimulus concentrations. This has been substantially demonstrated in a study which explored the unlikely response of a food volatile-sensitive ORN of Drosophila melanogaster to the sex pheromone of female silk moths, Bombyx mori 44 . Careful chemical analyses and the use of GC coupled to SSR (GC-SSR) demonstrated that a minute (< 1%) content of plant volatiles in the moth pheromone sample was responsible of the unexpected response of D. melanogaster ORNs.…”

Section: Discussionmentioning

confidence: 98%

A closer look at sex pheromone autodetection in the Oriental fruit moth

Pérez-Aparicio

Ammagarahalli²,

Gemeno

2022

Sci Rep

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Female moths emit sex pheromone to attracts males, and although they are not attracted to their own sex pheromone, they appear to detect it as it affects their behavior. In order to elucidate the mechanism of pheromone “autodetection” we compared responses of olfactory receptor neurons (ORNs) of male and female Grapholita molesta, a species with reported pheromone autodetection. Two concentrations of the major (Z8-12:Ac) and minor (E8-12:Ac) sex pheromone components, a plant-volatile blend containing methyl salicylate, terpinyl acetate and (E)-β-farnesene, and the male-produced hair-pencil (i.e., courtship) pheromone (ethyl trans-cinnamate) were tested in 45 male and 305 female ORNs. Hierarchical cluster analysis showed radically different peripheral olfactory systems between sexes that could be linked to their specific roles. In males 63% of the ORNs were tuned specifically to the major or minor female sex pheromone components, and 4% to the plant volatile blend, while the remaining 33% showed unspecific responses to the stimulus panel. In females 3% of the ORNs were specifically tuned to the male hair-pencil pheromone, 6% to the plant volatile blend, 91% were unspecific, and no ORN was tuned their own sex pheromone components. The lack of sex pheromone-specific ORNs in females suggests that they are not able to discriminate pheromone blends, and thus pheromone autodetection is unlikely in this species. We discuss our results in the context of the methodological limitations inherent to odor stimulation studies.

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“…This unspeci c activation could falsely signal the brain the presence of non-pheromone odors which could then trigger behavioral responses that the sex pheromone at natural concentrations would never do. Furthermore, at high stimulus concentrations small impurities present in synthetic compounds may cause false positives, especially if they are more volatile than the target compound [44].…”

Section: Discussionmentioning

confidence: 99%

A Closer Look At Sex Pheromone Autodetection In The Oriental Fruit Moth

Pérez-Aparicio

Ammagarahalli²,

Gemeno

2022

Preprint

View full text Add to dashboard Cite

Female moths emit sex pheromone to attracts males, and although they are not attracted to their own sex pheromone, they appear to detect it because it affects their behavior. In order to elucidate the mechanism of pheromone "autodetection" we compared responses of olfactory receptor neurons (ORNs) of male and female Grapholita molesta, a species with reported pheromone autodetection. Two concentrations of the major (Z8-12:Ac) and minor (E8-12:Ac) sex pheromone components, a plant-volatile blend containing methyl salicylate, terpinyl acetate and (E)-β-farnesene, and the male-produced hair-pencil (i.e., courtship) pheromone (ethyl trans-cinnamate) were tested in 45 male and 305 female ORNs. Hierarchical cluster analysis showed radically different peripheral olfactory systems between sexes that could be linked to their specific ecological roles. In males 63% of the ORNs were tuned specifically to the major and minor female sex pheromone components, and 4% to the plant volatile blend, while the remaining 33% showed unspecific responses. In females 3% of the ORNs were specifically tuned to the male hair-pencil pheromone, 6% to the plant volatile blend, 91% were unspecific, and no ORN was tuned their own sex pheromone components. The lack of sex pheromone-specific ORNs in females suggests that they are not able to discriminate pheromone blends, and thus pheromone autodetection is unlikely in this species.

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False positives from impurities result in incorrect functional characterization of receptors in chemosensory studies

Cited by 10 publications

References 37 publications

Olfaction in Tephritidae: a balance between detection and discrimination

Olfaction in Tephritidae: a balance between detection and discrimination

A closer look at sex pheromone autodetection in the Oriental fruit moth

A Closer Look At Sex Pheromone Autodetection In The Oriental Fruit Moth

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