False-positive troponin I elevation in a newborn with neonatal encephalopathy

Caredda, Elisabetta; Rosso, Roberto; Capristo, Carlo; Montaldo, Paolo

doi:10.1136/bcr-2019-229217

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…The first report of antibody interference was published in the early 1970s and concerned false positive test results due to endogenous anti-guinea-pig antibodies in human blood donors 5 . Almost 50 years later, new interference reports are still being submitted from all over the world 6–9 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

Bergman

Larsson

Hansson-Hamlin

et al. 2019

Sci Rep

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Immunoassays are widely used for detection and quantification of analytes in biological samples, but are vulnerable to analytical errors caused by interfering sample substances. Of particular interest are endogenous anti-animal antibodies that may bind to the immunoassay antibodies and cause erroneous test results. This phenomenon is a hazard to patient safety in both human and veterinary medicine. Here, we demonstrate that anti-mouse antibodies in dogs bind selectively to different regions of the murine IgG molecule, cross-react with IgG from different species, and consist of all major antibody classes present in canine serum (IgA, IgG and IgM). The antibody characteristics varied among individuals and their prevalence differed between two dog breeds. The selective binding to different IgG regions suggests that the antibodies might not originate from immunization through exposure to mice or other species. These findings show that canine anti-mouse antibodies are highly heterogeneous in nature and therefore require a combination of strategies to be counteracted.

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Section: Introductionmentioning

confidence: 99%

Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

Bergman

Larsson

Hansson-Hamlin

et al. 2019

Sci Rep

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False positive high-sensitivity cardiac troponin I elevation in a newborn

Domenis

Mauro²,

Paviotti³

et al. 2022

Clinica Chimica Acta

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Unexpected high troponin T and I values in a child with hypertrophic cardiomyopathy and acute chest pain: a case report

Avezaath

Nijenhuis

Kobold

2023

European Heart Journal - Case Reports

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Background Elevated troponin T and/or troponin I can be ascribed to multiple causes, mostly resulting from cardiac tissue damage and in lesser numbers resulting from non-cardiac related causes. The presence of macrotroponins is easily overlooked with potentially negative consequences. Case Summary This case report presents a case study of a 12 year old child known to have a MYH7-gene associated hypertrophic cardiomyopathy with acute chest pain combined with an unexpected high troponin T and I. A cardiac cause was deemed unlikely after additional investigation, as these showed no abnormalities. After consulting the laboratory specialist it could be concluded that the high troponin T and I were a result of macrotroponin complexes, a protein complex consisting of circulating protein and endogenous autoantibodies against that protein, resulting in elevated values with misguiding and uncertain clinical significance. Discussion Awareness of the existence of macrotroponins could have prevented costly diagnostics and prolonged hospital admission with grave psychological impact, especially in children.

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False-positive troponin I elevation in a newborn with neonatal encephalopathy

Cited by 4 publications

References 17 publications

Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

False positive high-sensitivity cardiac troponin I elevation in a newborn

Unexpected high troponin T and I values in a child with hypertrophic cardiomyopathy and acute chest pain: a case report

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