False-positive intercellular cement substance antibodies due to group A/B red cell antibodies: frequency and approach

Lee, Frederick J.; Silvestrini, Roger; Fulcher, David A.

doi:10.3109/00313025.2010.508792

Cited by 15 publications

(7 citation statements)

References 25 publications

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“…The specificity has been reported to be 100 % . On the other hand, antibodies against blood group antigens A and B, which are present in approximately 10 % of sera from healthy blood donors, show a virtually identical pattern to pemphigus autoantibodies . By adding soluble A/B antigen or erythrocytes from an AB‐positive donor, such undesirable intercellular reactivity may be eliminated in most sera .…”

Section: Diagnosis Of Pemphigus Vulgaris/pemphigus Foliaceusmentioning

confidence: 99%

S2k guideline for the diagnosis of pemphigus vulgaris/foliaceus and bullous pemphigoid

Schmidt

Goebeler

Hertl

et al. 2015

J Deutsche Derma Gesell

107

130

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Section: Diagnosis Of Pemphigus Vulgaris/pemphigus Foliaceusmentioning

confidence: 99%

S2k guideline for the diagnosis of pemphigus vulgaris/foliaceus and bullous pemphigoid

Schmidt

Goebeler

Hertl

et al. 2015

J Deutsche Derma Gesell

107

130

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“…The use of commercially available BIOCHIP containing only the DSG1 and DSG3 substrate assay would assist in differentiating pemphigus from a non-specific blood group antibody pattern found on standard indirect immunofluorescence on primate oesophageal tissue. 19 Commercial neutralising antibodies have been inefficient in removing this non-specific staining, highlighting the importance of specific antibody testing to DSG1 and DSG3 when this staining pattern is noted on standard monkey oesophagus-based assays. The BIOCHIP also makes available the diagnostic test salt split skin which has not been routinely easily accessible in some parts of Australia.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the EUROIMMUN Dermatology Profile ELISA to the novel BIOCHIP Mosaic 7 for the diagnosis of immunobullous skin disease

Simpson

Scardamaglia

Kok³

et al. 2021

Aust J Dermatology

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Background:The BIOCHIP is an indirect immunofluorescence diagnostic investigation which identifies multiple autoantibodies with a mosaic panel of target antigen-specific substrates in a single incubation field. The EUROIMMUN Dermatology Profile ELISA allows simultaneous investigation of the six most important autoantibodies in bullous autoimmune dermatoses. Evaluation of the BIOCHIP Mosaic 7, compared to that of the EUROIMMUN Dermatology Profile ELISA, when used as a diagnostic investigation in pemphigus and pemphigoid, was undertaken in an Australian cohort. Methods:The serum of 27 patients was analysed including patients with pemphigus vulgaris (n = 10), pemphigus foliaceous (n = 4), bullous pemphigoid (n = 8), mucous membrane pemphigoid (n = 3) and negative controls (n = 2). Results of the BIOCHIP were compared with the EUROIMMUN Dermatology Profile ELISA, as well as with histology, direct immunofluorescence and indirect immunofluorescence.Results: In pemphigus vulgaris, sensitivity & specificity for the BIOCHIP Mosaic 7 were 100% and 94.1%, comparable to that of the EUROIMMUN Dermatology Profile ELISA with 80% sensitivity and 100% specificity. In bullous pemphigoid, sensitivity of the BIOCHIP was 87.5% and sensitivity of the EUROIMMUN Dermatology ELISA profile was 75%, whilst specificities for both diagnostic methods were 100% in our limited cohort. There was substantial or almost perfect concordance between the BIOCHIP Mosaic 7 and EUROIMMUN Dermatology Profile ELISA for pemphigus vulgaris and bullous pemphigoid. Conclusion:The BIOCHIP Mosaic 7 is a rapid, reliable diagnostic investigation in pemphigus and bullous pemphigoid. Results indicate it is comparable to the EUROIMMUN Dermatology Profile ELISA, whilst also providing additional testing with salt split skin, on one field.

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“…Blood group antibodies have been shown to cross-react with antibodies associated with Sjogren syndrome and pemphigus vulgaris, causing false-positive tests for these conditions. 27,28 If O-negative blood type is a novel risk factor for false-positive rapid HIV tests, blood group antibodies may cross-react with the HIV antibodies of the diagnostic test.…”

Section: Discussionmentioning

confidence: 99%

Factors Associated with False-Positive Results from Fingerstick OraQuick ADVANCE Rapid HIV 1/2 Antibody Test

Rifkin

Owens

Greenwald

2012

Journal of the International Association of Physicians in AIDS

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show abstract

False-positive intercellular cement substance antibodies due to group A/B red cell antibodies: frequency and approach

Cited by 15 publications

References 25 publications

S2k guideline for the diagnosis of pemphigus vulgaris/foliaceus and bullous pemphigoid

S2k guideline for the diagnosis of pemphigus vulgaris/foliaceus and bullous pemphigoid

Comparison of the EUROIMMUN Dermatology Profile ELISA to the novel BIOCHIP Mosaic 7 for the diagnosis of immunobullous skin disease

Factors Associated with False-Positive Results from Fingerstick OraQuick ADVANCE Rapid HIV 1/2 Antibody Test

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