False Positive Immunostaining ofLocustaNeurosecretory Cells with a Variety of Preimmune Sera

Tips, Ann; Schoofs, Liliane; Paemen, Liesbet; Hendrickx, Koen; Loof, Arnold De

doi:10.1006/gcen.1996.6869

Cited by 4 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This, however, is no guarantee for the real occurrence of LMCI‐I because false positive staining could have occurred. No preimmunesera were used in their experiment and it is known that a large number of preimmunesera raised in rabbits against peptides isolated from locust brains, stain cell populations and fibers in the central nervous system due to preexisting antibodies of the IgG class [60]. Our experiments, however, unequivocally prove the presence of LMCI‐I in the neurosecretory cells of the PI and in the CC.…”

Section: Discussionmentioning

confidence: 59%

Peptidomics of the pars intercerebralis–corpus cardiacum complex of the migratory locust, Locusta migratoria

Clynen¹,

Baggerman²,

Veelaert³

et al. 2001

European Journal of Biochemistry

Self Cite

147

View full text Add to dashboard Cite

The pars intercerebralis±corpora cardiaca system (PI±CC) of insects is the endocrinological equivalent of the hypothalamus±pituitary system of vertebrates. Peptide profiles of the pars intercerebralis and the corpora cardiaca were characterized using simple sampling protocols in combination with MALDI-TOF and electrospray ionization double quadrupole time of flight (ESI-Qq-TOF) mass spectrometric technologies. The results were compared with earlier results of conventional sequencing methods and immunocytochemical methods. In addition to many known peptides, several m/z signals corresponding to putative novel peptides were observed in the corpora cardiaca and/or pars intercerebralis. Furthermore, for a number of peptides evidence was provided about their localization and MALDI-TOF analysis of the released material from the corpora cardiaca yielded information on the hormonal status of particular brain peptides.

show abstract

Section: Discussionmentioning

confidence: 59%