False-Positive Carbapenem-Hydrolyzing Confirmatory Tests Due to ACT-28, a Chromosomally Encoded AmpC with Weak Carbapenemase Activity from Enterobacter kobei

Jousset, Agnès B; Oueslati, Saoussen; Bernabeu, Sandrine; Takissian, Julie; Creton, Elodie; Vogel, Anaïs; Sauvadet, Aimie; Cotellon, Garance; Gauthier, L.; Bonnin, Rémy A.; Dortet, Laurent; Naas, Thierry

doi:10.1128/aac.02388-18

Cited by 25 publications

(23 citation statements)

References 32 publications

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“…Very recently, Jousset et al showed that E. cloacae can chromosomally encode an AmpC enzyme of the type ACT-28. Kinetic parameters of purified ACT-28 revealed a slightly increased imipenem hydrolysis compared to that of ACT-1 [177]. CMY-10 was the first reported carbapenemase among plasmidic class C β-lactamases, and this enzyme was also a class C ESBL with extended substrate specificity for extended-spectrum cephalosporins [178].…”

Section: Emerging Ambler Class C Carbapenemasesmentioning

confidence: 96%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

2020

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Carbapenemases are β-lactamases belonging to different Ambler classes (A, B, D) and can be encoded by both chromosomal and plasmid-mediated genes. These enzymes represent the most potent β-lactamases, which hydrolyze a broad variety of β-lactams, including carbapenems, cephalosporins, penicillin, and aztreonam. The major issues associated with carbapenemase production are clinical due to compromising the activity of the last resort antibiotics used for treating serious infections, and epidemiological due to their dissemination into various bacteria across almost all geographic regions. Carbapenemase-producing Enterobacteriaceae have received more attention upon their first report in the early 1990s. Currently, there is increased awareness of the impact of nonfermenting bacteria, such as Acinetobacter baumannii and Pseudomonas aeruginosa, as well as other Gram-negative bacteria that are carbapenemase-producers. Outside the scope of clinical importance, carbapenemases are also detected in bacteria from environmental and zoonotic niches, which raises greater concerns over their prevalence, and the need for public health measures to control consequences of their propagation. The aims of the current review are to define and categorize the different families of carbapenemases, and to overview the main lines of their spread across different bacterial groups.

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Section: Emerging Ambler Class C Carbapenemasesmentioning

confidence: 96%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

2020

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“…Class C β-lactamases, also known as cephalosporinases, have been rarely reported as carbapenemases. To date, only two β-lactamases were described possessing a carbapenemase activity being CMY-10 and more recently ACT-28 ( 109 , 110 ).…”

Section: Minor Class C Carbapenemasesmentioning

confidence: 99%

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

et al. 2021

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Gram-negative bacteria, especially Enterobacterales, have emerged as major players in antimicrobial resistance worldwide. Resistance may affect all major classes of anti-gram-negative agents, becoming multidrug resistant or even pan-drug resistant. Currently, β-lactamase-mediated resistance does not spare even the most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. The dissemination of carbapenemases-encoding genes among Enterobacterales is a matter of concern, given the importance of carbapenems to treat nosocomial infections. Based on their amino acid sequences, carbapenemases are grouped into three major classes. Classes A and D use an active-site serine to catalyze hydrolysis, while class B (MBLs) require one or two zinc ions for their activity. The most important and clinically relevant carbapenemases are KPC, IMP/VIM/NDM, and OXA-48. However, several carbapenemases belonging to the different classes are less frequently detected. They correspond to class A (SME-, Nmc-A/IMI-, SFC-, GES-, BIC-like…), to class B (GIM, TMB, LMB…), class C (CMY-10 and ACT-28), and to class D (OXA-372). This review will address the genetic diversity, biochemical properties, and detection methods of minor acquired carbapenemases in Enterobacterales.

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“…Notably, E. nimipressuralis CIP 104980 (clinical), E. hormaechei ATCC 49162 (sputum of a male patient), E. ludwigii EN-119 (clinical) and E. kobei JCM 8580 (clinical) sequenced in this study encode well-defined T6SSs containing vgrG and hcp proteins as orphan components. Several studies have reported the niche, antibiotic resistance and virulence of E. kobei and E. ludwigii, which are most commonly isolated from human clinical specimens [28][29][30] . Although these strains are most commonly associated with clinical specimens, variations in loci containing regulatory proteins such as prpC or prpK, hcp and vgrG have been observed, showing that these strains encode functional T6SSs.…”

Section: Genome Wide Gene Prediction and Analysis Of Virulent Genes mentioning

confidence: 99%

“…This notable expression was observed in all Ecc-type strains with little variation. The imp operon is a conserved part of almost all T6SSs and actively participates in mediating hcp secretion 9,30 . The high expression of the imp operon and prpC under in vivo mimicking conditions revealed that the imp operon seems to play a very active role in virulence and host-microbe interactions and is fully functional.…”

Section: Genome Wide Gene Prediction and Analysis Of Virulent Genes mentioning

confidence: 99%

Genome-wide Analysis of Four Enterobacter cloacae complex type strains: Insights into Virulence and Niche Adaptation

Mustafa

Ibrahim

Rasheed

et al. 2020

Sci Rep

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Enterobacter cloacae complex (ecc) species are widely distributed opportunistic pathogens mainly associated with humans and plants. In this study, the genomes of clinical isolates including E. hormaechei, E. kobei, and E. ludwigii and non-clinical isolate including E. nimipressuralis were analysed in combination with the genome of E. asburiae by using the reference strain E. cloacae subsp. cloacae ATCC 13047; the Ecc strains were tested on artificial sputum media (ASM), which mimics the host, to evaluate T6SS genes as a case study. All five Ecc strains were sequenced in our lab. Comparative genome analysis of the Ecc strains revealed that genes associated with the survival of Ecc strains, including genes of metal-requiring proteins, defence-associated genes and genes associated with general physiology, were highly conserved in the genomes. However, the genes involved in virulence and drug resistance, specifically those involved in bacterial secretion, host determination and colonization of different strains, were present in different genomic regions. For example, T6SS accessory and core components, T4SS, and multidrug resistance genes/efflux system genes seemed vital for the survival of Ecc strains in various environmental niches, such as humans and plants. Moreover, the ASM hostmimicking growth medium revealed significantly high expression of T6SS genes, including PrpC, which is a regulatory gene of the T6SS, in all tested Ecc strains compared to the control medium. The variations in T6SS gene expression in ASM vs. control showed that the ASM system represents a simple, reproducible and economical alternative to animal models for studies such as those aimed at understanding the divergence of Ecc populations. In summary, genome sequencing of clinical and environmental Ecc genomes will assist in understanding the epidemiology of Ecc strains, including the isolation, virulence characteristics, prevention and treatment of infectious disease caused by these broad-host-range niche-associated species. The Enterobacter genus was reported in 1960 by Hormaeche and Edwards 1. The List of Prokaryotic Names with Standing in Nomenclature (LPSN) reports that 12 species and 2 subspecies are included in this genus. Among them, the species of the Enterobacter cloacae complex (Ecc) are opportunistic and can cause lower respiratory tract infections and bacteraemia 2,3. These bacteria are also notorious for causing nosocomial infections in intensive care units. It has also been reported that The human gastrointestinal tract is the most common endogenous reservoir for these bacteria 4,5. More recently, it has been noted that Ecc bacteria pose a threat to plants by causing internal decay of onion and yellowing disease of papaya and lucerne seeds in China 6. Molecular and biochemical analyses have shown that the Ecc contains 6 species: E. cloacae subsp. cloacae, E. asburiae, E. kobei, E. hormaechei, E. ludwigii and E. nimipressuralis. Little is known about the genome correlation among the Ecc strains and the frequency of diseases cause...

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False-Positive Carbapenem-Hydrolyzing Confirmatory Tests Due to ACT-28, a Chromosomally Encoded AmpC with Weak Carbapenemase Activity from Enterobacter kobei

Cited by 25 publications

References 32 publications

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

Genome-wide Analysis of Four Enterobacter cloacae complex type strains: Insights into Virulence and Niche Adaptation

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