False-negative results in immunoblot assay of serum IgA antibodies reactive with the 180-kDa bullous pemphigoid antigen: the importance of primary incubation temperature.

Pas, Hendrikus; Kloosterhuis, Guus J.; Jong, M. C. J. M. de; Jonkman, Marcel F.

doi:10.1046/j.1365-2133.2001.04528.x

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…initially reported the presence of an IgA autoimmune response against the 180 kDa antigen. Thus, the failure of previous attempts to detect IgA reacting with the 180 kDa antigen appears to be technical, rather than a true negative immunoreactivity to BP180 21 . IgA reactivity to the 180 kDa antigen may be more readily detectable if the primary incubation temperature is increased to 37 °C 21 …”

Section: Resultsmentioning

confidence: 99%

Linear IgA dermatosis with IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180): evidence for a distinct subtype

et al. 2004

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Section: Resultsmentioning

confidence: 99%

Linear IgA dermatosis with IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180): evidence for a distinct subtype

et al. 2004

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“…Thus, the failure of previous attempts to detect IgA reacting with the 180 kDa antigen appears to be technical, rather than a true negative immunoreactivity to BP180. 21 IgA reactivity to the 180 kDa antigen may be more readily detectable if the primary incubation temperature is increased to 37 °C. 21 In summary, this study, using purified and recombinant proteins, clearly shows that our LAD patient's serum contains IgA and IgG antibodies to BP180.…”

Section: Resultsmentioning

confidence: 99%

“…21 IgA reactivity to the 180 kDa antigen may be more readily detectable if the primary incubation temperature is increased to 37 °C. 21 In summary, this study, using purified and recombinant proteins, clearly shows that our LAD patient's serum contains IgA and IgG antibodies to BP180. These data further confirm that the clinical features of LAD and BP are not dependent solely on the nature of the autoantigen; the immunoglobulin subclass also plays a determining role.…”

Section: Resultsmentioning

confidence: 99%

Granuloma multiforme

et al. 2004

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CASE 1: A 51-year-old woman presented with skin-colored annular lesions on the upper chest, neck, and dorsa of the hands of 1-year duration. The lesions initially started as small papular lesions, which gradually evolved to form large annular and polycyclic lesions. Initially, the lesions were associated with a burning sensation and pruritus. She had no other systemic complaints. Examination revealed 5-6 annular, polycyclic lesions distributed over the upper chest, neck, and dorsa of both hands, varying in size from 1 to 5 cm. All the lesions had a prominent erythematous to skin-colored, irregular, papular border which was firm in consistency (Figs 1 and 2). Central clearing and minimal atrophy were evident in larger lesions. There was no sensory loss or peripheral nerve thickening. Clinical possibilities entertained were granuloma annulare, granuloma multiforme, and annular sarcoid. Routine investigations, including hemogram, renal and liver functions, blood sugar levels, chest X-ray, and urine examination, were within normal limits. A skin biopsy taken from the edge of a lesion revealed foci of collagen degeneration surrounded by an inflammatory infiltrate composed of many histiocytes and multinucleated giant cells. In addition, there were perivascular and periadnexal lymphocytic aggregates. No acid-fast bacilli (AFB) were detected. These findings were consistent with a diagnosis of granuloma multiforme (Fig. 3). CASE 2: A 47-year-old man presented with annular skin-colored lesions associated with a mild burning sensation of 8 months' duration. On examination, 2-3 annular, arciform lesions were distributed over the upper chest and neck. The lesions ranged in size from 2 to 7 cm in diameter, were irregular in shape, and were rimmed by a well-defined raised papular border. Again, there was no sensory loss or peripheral nerve thickening. Histopathology of the skin from the edge of the lesion showed multiple areas of histiocytic granulomas with focal necrobiosis and prominent multinucleated giant cells, findings consistent with granuloma multiforme.

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Analysis of Antigens Targeted by Circulating IgG and IgA Antibodies in Patients With Mucous Membrane Pemphigoid Predominantly Affecting the Oral Cavity

Carrozzo

Cozzani

Broccoletti

et al. 2004

Journal of Periodontology

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False-negative results in immunoblot assay of serum IgA antibodies reactive with the 180-kDa bullous pemphigoid antigen: the importance of primary incubation temperature.

Abstract: Primary incubation at room temperature may result in false-negative results in the IgA-BP180 immunoblot assay.

Cited by 4 publications

References 14 publications

Linear IgA dermatosis with IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180): evidence for a distinct subtype

Linear IgA dermatosis with IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180): evidence for a distinct subtype

Granuloma multiforme

Analysis of Antigens Targeted by Circulating IgG and IgA Antibodies in Patients With Mucous Membrane Pemphigoid Predominantly Affecting the Oral Cavity

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