False Negative Results in High Viremia Parvovirus B19-Samples Tested with Real-Time PCR

Abstract

Extremely high viremia is observed during some viruses infection, especialy in immunocompromised patients. False negative results of Parvovirus B 19 DNA tests performed with real-time PCR in high viremic samples are reported. The way of fluorescence diagrams analysis and algorithm of positive result confirmation to exclude such phenomenon are proposed.

Multicenter evaluation of a commercial multiplex polymerase chain reaction test for screening plasma donations for parvovirus B19 DNA and hepatitis A virus RNA

“…The evaluating laboratories were able to identify such results by an examination of the amplification results and to resolve the issue by testing the invalid pool in dilution. A dilution resulting in a concentration of less than log 8.48 IU/mL would give a valid result, which allowed further deconstruction of the pool 38 …”

Interesting Presentation of Oral Herpes Simplex Virus in a High-Risk Patient

“…A potential explanation for false-negative test results are inhibitors such as heme factor, hemin, bilirubin, bile salts, lactoferrin, and immunoglobulins, as they interfere with PCR, leading to false-negative results [3]. Alternatively, falsely negative PCR results have been documented in patients with immunocompromised states in the setting of high viral loads [6]. Although HSV is self-limiting, acyclovir is one of the first-line treatments used for symptom resolution of acute flare-ups and chronic management [2].…”