Failure to observe fluoroscopic contrast agent in mouse hind limb

Terry, Tracee; Smith, Ray D.; Khitha, Jayant; Barlow, Shayne C.

doi:10.1038/laban1105-19

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…5,18 Briefly, the thoracic aorta was ligated with 5.0 silk suture material and cannulated caudal to the ligation using PE50 tubing held in place with a silk ligature. Mice were placed on the ventral detector of an OEC Series 9600 C-arm fluoroscope (OEC Medical Systems) for angiographic imaging.…”

Section: Hind Limb Angiographymentioning

confidence: 99%

Sildenafil Promotes Ischemia-Induced Angiogenesis Through a PKG-Dependent Pathway

Senthilkumar

Smith

Khitha

et al. 2007

ATVB

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Background— Peripheral artery disease (PAD) is a prevalent cardiovascular disorder that results in tissue ischemia which can progress to critical limb ischemia. Restoration of tissue perfusion in the setting of chronic ischemia through stimulation of arteriogenesis and angiogenesis remains a key therapeutic target for PAD. However, experimental therapeutics, including growth factor and gene therapy, have had little clinical success indicating the need for a better understanding of molecular pathways required for therapeutic angiogenesis. Methods and Results— Here we report that phosphodiesterase-5 inhibition by sildenafil significantly increases vascular perfusion, tissue blood flow, and vascular density during chronic ischemia of the mouse hind limb. Importantly, sildenafil therapy did not alter any of these parameters in nonischemic limbs. Sildenafil increased tissue cGMP levels independently of increases in nitric oxide production, and sildenafil therapy stimulated angiogenesis in ischemic limbs of eNOS −/− and iNOS −/− mice. Lastly, sildenafil-mediated angiogenic activity was blocked by inhibition of protein kinase G using the PKG antagonist DT-3. Conclusions— These data demonstrate that sildenafil therapy results in increased angiogenic activity through a PKG-dependent pathway that is independent of nitric oxide production or NOS activity and identify the angiogenic therapeutic potential of sildenafil for critical limb ischemia.

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Section: Hind Limb Angiographymentioning

confidence: 99%