Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development

Fu, Xinyu; Yang, Hannah; Pangestu, Febriana; Nikolau, Basil J.

doi:10.1104/pp.19.01162

Cited by 25 publications

(36 citation statements)

References 90 publications

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“…Because acetaldehyde showed a completely different labelling pattern compared with acetic acid, we concluded that the biosynthesis of acetic acid in the chloroplast did not involve pyruvate and acetaldehyde, as recently proposed by Fu et al . (2020) and others (e.g. Lin & Oliver, 2008) but needed another biosynthetic origin (point 3; Fig.…”

Section: Discussionmentioning

confidence: 99%

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

et al. 2021

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Summary The effect of drought on the interplay of processes controlling carbon partitioning into plant primary and secondary metabolisms, such as respiratory CO2 release and volatile organic compound (VOC) biosynthesis, is not fully understood. To elucidate the effect of drought on the fate of cellular C sources into VOCs vs CO2, we conducted tracer experiments with 13CO2 and position‐specific 13C‐labelled pyruvate, a key metabolite between primary and secondary metabolisms, in Scots pine seedlings. We determined the stable carbon isotope composition of leaf exchanged CO2 and VOC. Drought reduced the emission of the sesquiterpenes α‐farnesene and β‐farnesene but did not affect 13C‐incorporation from 13C‐pyruvate. The labelling patterns suggest that farnesene biosynthesis partially depends on isopentenyl diphosphate crosstalk between chloroplasts and cytosol, and that drought inhibits this process. Contrary to sesquiterpenes, drought did not affect emission of isoprene, monoterpenes and some oxygenated compounds. During the day, pyruvate was used in the TCA cycle to a minor degree but was mainly consumed in pathways of secondary metabolism. Drought partly inhibited such pathways, while allocation into the TCA cycle increased. Drought caused a re‐direction of pyruvate consuming pathways, which contributed to maintenance of isoprene and monoterpene production despite strongly inhibited photosynthesis. This underlines the importance of these volatiles for stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

et al. 2021

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“…A key component of acetate metabolism in plants responsible for the conversion of acetate fermentation products (acetaldehyde, ethanol, and acetate) to acetyl-CoA derived metabolites [ 24 ]. Recently, studies with Arabidopsis double mutants of ACETYL-COA SYNTHETASE (ACS) in plastids and ACETATE NON-UTILIZING1 (ACN1) in peroxisomes (which enables the incorporation of acetate into organic acids and amino acids) showed severe morphological and metabolic phenotypes including delayed growth and sterility associated with hyperaccumulation of cellular acetate and decreased accumulation of acetyl-CoA-derived intermediates of central metabolism [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Are Methanol-Derived Foliar Methyl Acetate Emissions a Tracer of Acetate-Mediated Drought Survival in Plants?

Dewhirst

Lei

Afseth

et al. 2021

Plants

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Upregulation of acetate fermentation in plants has recently been described as an evolutionarily conserved drought survival strategy, with the amount of acetate produced directly correlating to survival. However, destructive measurements are required to evaluate acetate-linked drought responses, limiting the temporal and spatial scales that can be studied. Here, 13C-labeling studies with poplar (Populus trichocarpa) branches confirmed that methyl acetate is produced in plants from the acetate-linked acetylation of methanol. Methyl acetate emissions from detached leaves were strongly stimulated during desiccation, with total emissions decreasing with the leaf developmental stage. In addition, diurnal methyl acetate emissions from whole physiologically active poplar branches increased as a function of temperature, and light-dark transitions resulted in significant emission bursts lasting several hours. During experimental drought treatments of potted poplar saplings, light-dark methyl acetate emission bursts were eliminated while strong enhancements in methyl acetate emissions lasting > 6 days were observed with their initiation coinciding with the suppression of transpiration and photosynthesis. The results suggest that methyl acetate emissions represent a novel non-invasive tracer of acetate-mediated temperature and drought survival response in plants. The findings may have important implications for the future understanding of acetate-mediated drought responses to transcription, cellular metabolism, and hormone signaling, as well as its associated changes in carbon cycling and water use from individual plants to whole ecosystems.

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“…However, identification of acetate esters of phenylpropenes in the leaf essential oils of these species has never been reported in the literature [37,[61][62][63]. Acetic acid is a precursor of acetyl-CoA, which is a metabolic intermediate responsible for the biosynthesis of acetylated metabolites and isoprenoids in plants [64,65]. Hence, we hypothesized that biosynthesis of acetate esters of phenylpropenes is associated with pooling of acetic acid, which is a part of specialized metabolism in P. betle leaves.…”

Section: Hypothesesmentioning

confidence: 97%

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Osman

Lee

Sarbini

et al. 2021

Plants

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The differences in pungency of “sirih” imply the probable occurrence of several variants of Piper betle L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian P. betle variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via 1H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the 1H-NMR spectral data showed quantitative differences in the metabolite profiles of “sirih melayu” and “sirih india” and revealed an ambiguous group of samples with low acetic acid content, which was identified as Piper rubro-venosum hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of P. betle samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into “sirih melayu” and “sirih india” groups. Higher abundance of chavicol acetate was consistently found to be characteristic of “sirih melayu”. The present research has provided preliminary evidence supporting the notion of occurrence of two P. betle variants in Malaysia based on chemical profiles, which may be related to the different genders of P. betle.

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Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development

Cited by 25 publications

References 90 publications

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

Are Methanol-Derived Foliar Methyl Acetate Emissions a Tracer of Acetate-Mediated Drought Survival in Plants?

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

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