Failure to detect eye muscle membrane specific autoantibodies in Graves' ophthalmopathy.

Sikorska, Hanna; Wall, Jack R.

doi:10.1136/bmj.291.6495.604-b

Cited by 10 publications

(4 citation statements)

References 1 publication

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“…Circulating autoantibodies directed against various retroocular tissue preparations (sometimes called ophthalmic Igs) have been described previously (8)(9)(10)(11) indentifiable cellular population in the ocular tissue preparations and cross-react with antigens from other tissues (11). One report of human eye muscle membranespecific antibodies in serum of patients with active severe GO (10) has been questioned by other investigators (18). The possible existence of circulating antibodies to skin fibroblasts was shown by the isolation of a monoclonal antibody to the TSH receptor from the peripheral lymphocytes of a patient with GO (19) which stimulated collagen synthesis by skin fibroblasts.…”

Section: Discussionmentioning

confidence: 96%

Human Retroocular Fibroblastsin Vitro: A Model for the Study of Graves' Ophthalmopathy

Bahn

Gorman

Woloschak

et al. 1987

The Journal of Clinical Endocrinology & Metabolism

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Orbital connective tissue and extraocular muscles are histologically abnormal in patients with Graves' ophthalmopathy (GO). Although extraocular muscles have been extensively studied as a target for autoimmune attack in the disease, no system has been available to study retroocular fibroblasts in GO. We developed a technique to isolate fibroblasts from minced explants of connective tissue removed in the course of transantral orbital decompression surgery for GO. Postmortem specimens from individuals without evidence of thyroid disease were obtained from the same anatomical site. Confluent cells could be passaged at least 10 times with stable morphology and frozen with greater than 75% viability. An enzyme-linked immunosorbent assay was used to study the in vitro binding of serum immunoglobulin G (IgG) to these cells. We found no difference in the degree of specific binding between GO cells and cells from normal individuals. However, there was significantly greater binding of IgG from GO sera than of that from normal serum to retroocular GO fibroblasts. These results suggest that serum IgG autoantibodies against normal retroocular fibroblasts are present in some GO patients.

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Section: Discussionmentioning

confidence: 96%

Human Retroocular Fibroblastsin Vitro: A Model for the Study of Graves' Ophthalmopathy

Bahn

Gorman

Woloschak

et al. 1987

The Journal of Clinical Endocrinology & Metabolism

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“…Many investigators have searched for and found, antibodies to extraocular muscle cell membranes in patients with Graves' ophthalmopathy. The pathogenic potential of anti extraocular muscle antibodies was demonstrated by Wang et al [13] and Hiromatsu et al [14] but they did not Graves' ophthalmopathy 27 report testing their antibody in an ELISA or a RIA with a membrane preparation.…”

Section: Antibodies To Extraocular Muscle Antigensmentioning

confidence: 99%

“…Other authors [5,6,8,9,10,12] resorted to the use of extraocular muscles of non-human origin to avoid aspecific binding of anti-human immunoglobulin antibodies. Sikorska et al [27,28] demonstrated that a human eye muscle membrane preparation reacted with an anti-human Ig antiserum.…”

Section: Specificity Of the Antibodies To Extraocular Muscle Antigensmentioning

confidence: 99%

Graves' ophthalmopathy: What is the evidence for extraocular muscle specific autoantibodies

Gaag¹,

Vernimmen²,

Fiebelkorn³

et al. 1990

Int Ophthalmol

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Graves' ophthalmopathy is thought to be an autoimmune disease. Whether this disease is organ specific or one of the manifestations of the closely linked Graves' thyroid disease is still a matter of speculation. Many papers describe autoimmunity to orbital antigens, in particular to extraocular muscles, in patients with Graves' ophthalmopathy. In this paper we have tried to give an overview of the evidence for circulating antibodies against extraocular muscle antigens and to discuss their significance in relation to the immunopathogenesis of the disease.

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“…The methods used have been enzyme-linked immunosorbent assay (Kodama et al, 1982;Kendall-Taylor et al, 1984;Ahmann et al, 1987), staphylococcal protein A binding procedure (Faryna et al 1985), or immunofluorescence technique (Mengistu et al, 1986). However, difficulties in reproducing some of these findings have been reported (Sikorska & Wall, 1985;Wall & Kuroki, 1985).…”

mentioning

confidence: 99%

CORRELATION OF ORBITAL COMPUTED TOMOGRAPHY AND ANTIBODIES IN PATIENTS WITH HYPERTHYROID Graves'DISEASE

Chang

Huang

Chang

et al. 1990

Clinical Endocrinology

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The purpose of this study was to elucidate the incidence of orbital changes in computed tomography, and the relationships of orbital changes with eye-muscle antibody (EMAb), thyrotrophin-binding inhibitor immunoglobulin (TBII), antithyroglobulin antibody (TgAb) and anti-thyroid microsomal antibody (MsAb), in 55 consecutive patients with hyperthyroid Graves' disease. EMAb was demonstrated by an immunoperoxidase technique. The results showed that the prevalence of orbital fat increase, extraocular muscle enlargement, lacrimal gland enlargement and optic nerve swelling were 54.5, 41.8, 21.8 and 16.5% respectively. There were 32.7% of patients without any orbital changes. The patients with extraocular muscle enlargement, lacrimal gland enlargement and optic nerve swelling had higher titres of EMAb than the patients without these orbital changes. The patients with lacrimal gland enlargement had a lower TBII index than those without. There was no difference in TgAb or MsAb between the patients with extraocular muscle enlargement, lacrimal gland enlargement, optic nerve swelling or orbital fat increase and those patients without these orbital changes. The patients with extraocular muscle enlargement, optic nerve swelling and orbital fat increase had a higher degree of exophthalmos than the patients without these orbital changes. EMAb was negatively correlated with TBII. However, there was no correlation between EMAb and TgAb or MsAb. The degree of exophthalmos was positively correlated with EMAb and negatively correlated with age. There was no correlation between exophthalmos and TBII, TgAb or MsAb. In conclusion, although the pathogenesis of orbital changes and hyperthyroidism is different, a close link between these two entities is suggested by the high incidence of orbital changes in hyperthyroid Graves' disease.(ABSTRACT TRUNCATED AT 250 WORDS)

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Failure to detect eye muscle membrane specific autoantibodies in Graves' ophthalmopathy.

Abstract: and successful treatment cannot always be guaranteed.We would therefore suggest that patients who have radical chemotherapy for P vivax should be warned of the possibility of relapse up to two years after leaving an endemic area.

Cited by 10 publications

References 1 publication

Human Retroocular Fibroblastsin Vitro: A Model for the Study of Graves' Ophthalmopathy

Human Retroocular Fibroblastsin Vitro: A Model for the Study of Graves' Ophthalmopathy

Graves' ophthalmopathy: What is the evidence for extraocular muscle specific autoantibodies

CORRELATION OF ORBITAL COMPUTED TOMOGRAPHY AND ANTIBODIES IN PATIENTS WITH HYPERTHYROID Graves'DISEASE

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