Failure to detect Borna disease virus infection in peripheral blood leukocytes from humans with psychiatric disorders

Ja, Richt; Rc, Alexander; Herzog, S.; Dc, Hooper; Kean, Rhonda; Spitsin, Sergei; Bechter, Karl; Schüttler, R.; Feldmann, H.; Heiske, Andreas; Fu, ZF; Dietzschold, B; Rott, R.; Koprowski, Hilary

doi:10.3109/13550289709015807

Cited by 57 publications

(32 citation statements)

References 12 publications

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“…Recently, Kubo et al (1997), using the identical primers, also demonstrated no association between BDV infection and psychiatric disorders among Japanese patients. In addition, three other groups have similarly failed to detect BDV-speci®c RNA in PBMC and/or brain biopsy samples from patients with schizophrenia and affective disorders (Sierra-Honigmann et al, 1995;Lieb et al, 1997;Richt et al, 1997). Neither infectious virus nor BDV-speci®c RNA was detectable in PBMC of psychiatric patients who exhibited serum antibodies reactive to the p24 and/ or p38 antigens of BDV (Sierra-Honigmann et al, 1995;Richt et al, 1997).…”

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confidence: 97%

“…By contrast, genomic analyses of BDV in psychiatric patients have yielded con¯icting results. Whereas researchers in Germany and Japan have detected BDV nucleic acid in peripheral blood mononuclear cells (PBMC) of patients with schizophrenia and affective disorders (Bode et al, 1995;Kishi et al, 1995;Sauder et al, 1996;Igata-Yi et al, 1996), other investigators have been unsuccessful in detecting BDV genomic sequences in brain tissue, cerebrospinal¯uid or PBMC (Sierra-Honigmann et al, 1995;Richt et al, 1997). Using nested reverse transcription-polymerase chain reaction (RT ± PCR), we analyzed RNA extracted from PBMC of Korean patients with schizophrenia, bipolar affective disorders and major depression for a 391-nucleotide, highly conserved region of the p24 protein-encoding ORF II of BDV.…”

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Failure to demonstrate Borna disease virus genome in peripheral blood mononuclear cells from psychiatric patients in Korea

Kim¹,

Kim²,

Choi³

et al. 1999

J Neurovirol

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confidence: 97%

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confidence: 99%

Failure to demonstrate Borna disease virus genome in peripheral blood mononuclear cells from psychiatric patients in Korea

Kim¹,

Kim²,

Choi³

et al. 1999

J Neurovirol

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“…Richt et al reported that they failed to detect BDV transcripts in peripheral blood cells from psychiatric patients. 21 Similarly, Kubo et al found no association of BDV infection and psychiatric disorders in the Japanese population while investigating the BDV-specific antibodies and BDV transcripts among psychiatric patients and normal controls. 22 Lieb et al also reported that they were unable to detect BDVspecific RNA in blood from psychiatric patients from Germany.…”

Section: Introductionmentioning

confidence: 99%

High seroprevalence of Borna virus infection in schizophrenic patients, family members and mental health workers in Taiwan

Chen

Yl²,

Fc³

et al. 1999

Mol Psychiatry

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Borna disease virus (BDV), a negative-strand RNA virus, has been reported to be associated with severe psychiatric disorders. The association is mainly based on the findings that patients with schizophrenia and depression have a higher seroprevalence rate of BDV-specific antibodies than controls. In addition, psychiatric patients were also found to have a higher detection rate of BDV transcripts in their blood than controls. By using an improved Western blot analysis, we first demonstrated that Chinese schizophrenic patients from Taiwan also have a higher seroprevalence of BDV-specific antibodies than controls (12.1% vs 2.9%, P Ͻ 0.001), providing support to the positive association between BDV and psychiatric disorders in our population. Because of the contagious nature of viral infection, we further examined patients' family members and mental health workers, who have close contact with patients. We found that both groups also have a higher seroprevalence of BDV-specific antibodies, 12.1% and 9.8%, respectively, than controls. This finding provides some evidence for a possible human-to-human transmission of Borna disease virus. Our finding needs further independent verification from other research groups and the clinical relevance of this preliminary observation deserves further study.

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“…Nevertheless, the role of BDV in human mental disorder is not without controversy, several groups presented contradictory results and indicated the possible methodological defects in previous serological and molecular biological studies. [16][17][18] Hence, the association of BDV and human mental disorders needs further clarification.…”

Section: Introductionmentioning

confidence: 99%

Detection of Borna disease virus RNA from peripheral blood cells in schizophrenic patients and mental health workers

Yl²,

et al. 1999

Mol Psychiatry

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Accumulating evidence suggests that Borna disease virus (BDV), a neurotropic, negativestranded RNA virus, might be associated with certain human mental disorders. Several research groups reported that psychiatric patients had a significantly higher prevalence of BDV serum antibodies than normal controls. In addition, a significantly higher presence of BDV RNA from peripheral blood cells was identified in mental patients than in controls. In our previous study, we first identified the presence of BDV serum antibodies in a cohort of Chinese schizophrenic patients from Taiwan, and we also demonstrated a significantly higher seroprevalence of BDV antibodies among schizophrenic patients than in non-psychiatric controls. Prompted by the positive seroepidemiological result, we set out to investigate the detection of BDV RNA from the peripheral blood cells of our schizophrenic patients. By using the reverse transcription-polymerase chain reaction (RT-PCR) method, 10 out of 74 Chinese schizophrenic patients from Taiwan were found to have BDV RNA in their blood cells, whereas only one out of 69 controls was positive. The BDV RNA detection rate among schizophrenic patients was significantly higher than that in controls (14% vs 1.4%, P Ͻ 0.01). Furthermore, we studied the BDV RNA detection rate among mental health workers, and seven out of 45 mental health workers were found to have positive results. The prevalence rate was significantly higher than that in normal controls (15% vs 1.4%, P Ͻ 0.001), which lends further support to our previous finding that mental health workers have a significantly higher presence of BDV serum antibodies. In summary, our data support the finding that BDV infection might be a contributory factor to the pathogenesis of schizophrenia in the Chinese population.

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Failure to detect Borna disease virus infection in peripheral blood leukocytes from humans with psychiatric disorders

Cited by 57 publications

References 12 publications

Failure to demonstrate Borna disease virus genome in peripheral blood mononuclear cells from psychiatric patients in Korea

Failure to demonstrate Borna disease virus genome in peripheral blood mononuclear cells from psychiatric patients in Korea

High seroprevalence of Borna virus infection in schizophrenic patients, family members and mental health workers in Taiwan

Detection of Borna disease virus RNA from peripheral blood cells in schizophrenic patients and mental health workers

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