Failure of Standard Treatment With Praziquantel in Two Returned Travelers With Schistosoma Haematobium Infection

Alonso, David; Muñóz, José; Gascón, Joaquím; Valls, María Eugenia; Corachán, M

doi:10.4269/ajtmh.2006.74.342

Cited by 125 publications

(89 citation statements)

References 15 publications

(22 reference statements)

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“…In order to prevent relapse and long term sequelae from insufficient treatment, it is important to achieve a laboratory confirmation of treatment success (Rabello et al 1997;Alonso et al 2006;Khurana et al 2005;Lambertucci et al 2000). Patients after therapy as well as patients after a long course of disease with spontaneous healing (''burnt Fig.…”

Section: Resultsmentioning

confidence: 99%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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Searching for a more sensitive and accurate marker for schistosomiasis diagnosis and treatment follow up is a potential necessity. Hereby, we evaluated usefulness of circulating free DNA as a marker for schistosomiasis diagnosis, assessing drug efficacy and monitoring the control interventions impact using SYBR green real-time PCR. A batch of mice were infected by 90 ± 10 Schistosoma mansoni cercariae. Starting from the 2nd day post infection (p.i.), groups of 2 or 3 mice were sacrificed every 3 days until 30 days p.i. The remaining animals were treated by a single dose of 400 mg/kg mefloquine and sacrificed in group at 5, 10, 21 days post treatment (35, 40, 51 days p.i.). Using SYBR green real time qPCR, pooled sera DNA were extracted and amplified. The results showed that, circulating free S. mansoni DNA was detected from the 2nd day post infection (p.i.) onwards with gradual decrease in the cycle threshold value C t which indicates the gradual elevation of the DNA level (Log quantity was 2.6-3.1 IU/ml), As the infection progressed, DNA quantity was increased(Log quantity was 6.29 IU/ml). Initial increase of circulating free DNA was observed 10 days post treatment (40 days p.i.) (Log quantity was 7.38 IU/ ml). That was followed by a progressive decrease in DNA level by the end of 21st day, post treatment (51 p.i.) (Log quantity 4.35 IU/ml). In conclusion, circulating free S. mansoni DNA is a reliable marker in the diagnosis of schistosomiasis and for assessing drug efficacy and monitoring the impact of control interventions.

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Section: Resultsmentioning

confidence: 99%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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“…At present, praziquantel-resistant schistosome populations do not seem to be posing a major problem for chemotherapy of human infections. However, reports of human infections that do not respond to standard praziquantel treatments suggest that resistance may be appearing in the field (Alonso et al 2006).…”

Section: Zoonotic Trematode Infections (A) Fasciolosismentioning

confidence: 99%

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Robinson

Dalton

2009

Phil. Trans. R. Soc. B

148

109

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Zoonotic infections are among the most common on earth and are responsible for .60 per cent of all human infectious diseases. Some of the most important and well-known human zoonoses are caused by worm or helminth parasites, including species of nematodes (trichinellosis), cestodes (cysticercosis, echinococcosis) and trematodes (schistosomiasis). However, along with social, epidemiological and environmental changes, together with improvements in our ability to diagnose helminth infections, several neglected parasite species are now fast-becoming recognized as important zoonotic diseases of humans, e.g. anasakiasis, several fish-borne trematodiasis and fasciolosis. In the present review, we discuss the current disease status of these primary helminth zoonotic infections with particular emphasis on their diagnosis and control. Advances in molecular biology, proteomics and the release of helminth genome-sequencing project data are revolutionizing parasitology research. The use of these powerful experimental approaches, and their potential benefits to helminth biology are also discussed in relation to the future control of helminth infections of animals and humans.

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“…This method does not eliminate transmission and therefore requires regular drug treatment to be sustainable. Although possible drug resistance in schistosomes has been reported (Gryseels et al, 2001;Alonso et al, 2006), it has not become a widespread phenomenon. However, with repeated selection pressure, there is a distinct probability that drug resistance will evolve in these organisms.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of Schistosoma mansoni: A robust, high-throughput method to assess multiple microsatellite markers from individual miracidia

Steinauer

Agola

Mwangi

et al. 2008

Infection, Genetics and Evolution

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Schistosomiasis is one of the major unconquered infectious diseases afflicting people of developing countries, particularly in Africa. A deeper understanding of the epidemiology of schistosomes is complicated by the intravascular location of adult worms which makes them routinely unavailable for study. Their progeny, miracidia, which are hatched from eggs that are passed in feces, are available and can provide valuable insights about human infections, but they are small in size, hindering robust molecular analyses. Here we present a new high-throughput technique to assess the genotypes at 21 previously published microsatellite loci for individual miracidia of S. mansoni. The 21 loci can be amplified in four multiplexed PCR reactions; however, enough template is produced for approximately six PCR reactions, which allows for additional PCR reactions for resampling or obtaining additional data. We validated this technique using a pedigree study employing laboratory crosses of S. mansoni from Kenya to obtain sets of parents and offspring. Of 23 loci examined, 21 loci were found to be reliable: false alleles were rare and missing alleles due to allelic dropout occurred at only two loci in approximately 5% of the offspring. The latter type of error can be further reduced by reamplification which is possible with our method. This technique is amenable to a 96-well format thus facilitating analysis of larger samples of miracidia, allowing more robust molecular epidemiological studies of S. mansoni to infer population size, population structure, gene flow, mating systems, speciation, and host race formation.

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Failure of Standard Treatment With Praziquantel in Two Returned Travelers With Schistosoma Haematobium Infection

Cited by 125 publications

References 15 publications

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Molecular epidemiology of Schistosoma mansoni: A robust, high-throughput method to assess multiple microsatellite markers from individual miracidia

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