Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm

Battaglia, David E.; Koehler, James K.; Klein, Nathan; Tucker, Michael

doi:10.1016/s0015-0282(97)81486-0

Cited by 123 publications

(94 citation statements)

References 16 publications

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“…To rescue cases that encounter problems in oocyte activation immediately following sperm-oocyte fusion, certain artificial activation procedures have been recommended: mechanical activation by a modified ICSI technique [8,9], electrical activation [10,11], chemical compounds such as strontium chloride [12,13], ionomycin [14], and calcium ionophores [15], and sequential treatment with calcium ionophore and puromycin [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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Purpose Fertilization failures have occurred repeatedly in reproductive centers after intracytoplasmic sperm injection (ICSI) and artificial oocyte activation (AOA) has been used to prevent it. This study was performed to investigate whether spermatozoan origin influences clinical outcomes of AOAwith a calcium ionophore. Methods A total of 185 ICSI cycles with a history of no or low fertilization was included in this retrospective study. The outcomes of AOA after ICSI were compared with ejaculatednormal, ejaculated-oligo-astheno-terato or extracted-testicular spermatozoa. Results There were significant differences between the previous standard ICSI cycles and AOA cycles in the rate of fertilization and clinical outcomes among cases with different sperm origins. Thirty-eight healthy babies (20 singles and 18 twins, 29 cycles) were successfully delivered, and no congenital birth defects were observed. Conclusions Most patients with a no or low fertilization history obtained an increased fertilization rate and a positive clinical outcome with AOA regardless of the origin of spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Yoon

Bae

Kim

et al. 2013

J Assist Reprod Genet

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“…Transmission electron micrographs of spermatozoon from a globozoospermic man demonstrate the typical round head, large vacuolated regions within the nucleus, absence of acrosome and an abnormal mid-piece and tail (Stone et al, 2000, Dam et al, 2007. Nuclear damage was also described with abnormal chromatin packaging (Larson et al, 2001) and mitochondria abnormalities (Battaglia et al, 1997). Morphological defects in human sperm cells raise suspicion for further anomalies within the sperm cell and could have implications in clinical practice.…”

Section: Treatment For Globozoospermiamentioning

confidence: 99%

“…Thus, ICSI is an only treatment option for these patients, but poor fertilization and only few baby births have been reported since the first live-birth from globozoospermia was reported in 1995 (Trokoudes et al, 1995). This poor fertilization rate can probably be explained by abnormal oocyte activation (Battaglia et al, 1997;Nardo et al, 2002). Low fertilization rates after ICSI indicate that these sperm do not have an ability to activate the oocyte.…”

Section: Treatment For Globozoospermiamentioning

confidence: 99%

Advances in Fertility Options of Azoospermic Men

Wu¹,

Gelety²,

Shi³

2012

Advances in Embryo Transfer

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“…The reasons for this phenomenon was considered to be a partial or complete inability of the spermatozoa to activate the oocytes, deficiency of sperm protamine, or the inability of the oocytes to decondense spermatozoa [1][2][3]. When the oocytes were activated using electroporation [4,5], calcium ionophore [6][7][8][9][10][11][12][13][14], or calcium ionophore and puromycin [15,16], followed by ICSI in women whose oocytes failed to fertilize in previous IVF cycles, some of them could form pronuclei. Recently, several studies were reported that strontium chloride (SrCl 2 ) treatment on infertile patients resulted in successful pregnancies and deliveries [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Live birth after SrCl2 oocyte activation in previous repeated failed or low fertilization rates after ICSI of frozen-thawed testicular spermatozoa: case report

Kim

Choi

Yang

et al. 2012

J Assist Reprod Genet

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Purpose To report a live birth resulting after strontium chloride (SrCl 2 ) oocyte activation in a couple with complete fertilization failure or low fertilization rates following intracytoplasmic sperm injection (ICSI) of frozen-thawed testicular spermatozoa. Methods The couple underwent ICSI of frozen-thawed testicular spermatozoa. After ICSI, the oocytes were artificially activated by SrCl 2 because the results of fertilization were not satisfactory in the previous cycles. The main outcome measures were fertilization, pregnancy, and birth. Results In the first and second cycles performed previously at another clinic, fertilization rates were 9.1 % and 0.0 %, respectively. In the third cycle, 31 metaphase II oocytes were retrieved. After sperm injection, all of the oocytes were stimulated using SrCl 2 for activation. Sixteen oocytes were fertilized (51.6 %), and a single embryo was transferred into the uterus on Day 3. A healthy girl weighing 2750 g was born at 40 weeks of gestation by caesarean section. Conclusions This result suggests that SrCl 2 could be useful for oocyte fertilization in case of repeated complete fertilization failure or low fertilization rates following ICSI of frozen-thawed testicular spermatozoa.

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Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm

Cited by 123 publications

References 16 publications

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

Advances in Fertility Options of Azoospermic Men

Live birth after SrCl2 oocyte activation in previous repeated failed or low fertilization rates after ICSI of frozen-thawed testicular spermatozoa: case report

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