Faecalibacterium prausnitzii phylotypes in type two diabetic, obese, and lean control subjects

Abstract

Faecalibacterium prausnitzii is one of the main butyrate producers in the healthy human gut. Information on its genetic diversity is lacking, although two genetic phylotypes have been differentiated. In the present study, F. prausnitzii phylotypes were examined in faeces of obese and type two diabetes with similar eating behaviour compared to a lean control group. The purpose of the study was to analyse if an excessive butyrate production induced by different F. prausnitzii phylotypes discriminates between obese developing type two diabetes or not. The faecal samples were analysed for the total abundance of F. prausnitzii 16S rRNA copies, fragment lengths polymorphism, high resolution melt curve analysis (HRM) and the butyryl-CoA:acetate CoA-transferase gene copies and melt curve variances. The diabetic group was found to differ significantly from the lean control group in the results of qPCR, butyryl-CoA:acetyate CoA-transferase gene melt curve, and HRM. F. prausnitzii phylotypes differed in obese with and without developed diabetes type two. Different phylotypes of F. prausnitzii may lead to differences in the inflammatory genesis in the host. F. prausnitzii phylotypes may have an influence on developing type two diabetes and might also act as starting points for prevention and therapy of obesity associated disease.

Prebiotics Reduce Body Fat and Alter Intestinal Microbiota in Children Who Are Overweight or With Obesity

“…Functional differences between genetic phylotypes of F prausnitzii with different capacities for butyrate production have also been observed with lean individuals having a genetic variant with a more moderate capacity for butyrate production compared with individuals with obesity and T2D. 57 In accordance with this, children with obesity had higher stool concentrations of butyrate compared with normal-weight controls. 58 There are some limitations to our study, including a reduced generalizability of our findings because of the primarily white and middle to high socioeconomic status of our participants.…”

Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations

“…Literature data are conflicting about the level of F. prausnitzii in obesity, with studies showing positive (Balamarugan et al, 2010), negative (Borgo et al, 2016) or no association (Feng et al, 2014). These contradictory results may be due to experimental factors such as small cohort sizes or the use of different primer sets, or may be explained by the existence of multiple F. prausnitzii phylotypes (Louis et al, 2009;Hippe et al, 2016). Indeed, Hippe and colleagues (2016) suggested that the two identified phylotypes display different physiological properties and seem to produce different amounts of butyrate in the gut.…”

Interactions between host genetics and gut microbiome in diabetes and metabolic syndrome

“…No universal microbe has been identified leading to the difference in weight gain, but, in various studies, associations of weight gain with different individual bacterial taxa, including Akkermansia muciniphila , Faecalibacterium prausnitzii [14], [59], [60], [61] and Lactobacillus reuteri and Roseburia intestinalis [57] have been observed. Some of the complexity in determining cause and effect is illustrated by a recent study by Goodrich and colleagues [62b], which demonstrated a strong association of BMI in humans with microbiota of the Christensenellaceae family.…”