Faecal virome of healthy chickens reveals a large diversity of the eukaryote viral community, including novel circular ssDNA viruses

Lima, Diane Alves de; Cibulski, Samuel Paulo; Finkler, Fabrine; Teixeira, Thaís Fumaco; Varela, Ana Paula Muterle; Cerva, Cristine; Loiko, Márcia Regina; Scheffer, Camila Mengue; Santos, Helton Fernandes dos; Mayer, Fabiana Quoos; Roehe, Paulo Michel

doi:10.1099/jgv.0.000711

Cited by 52 publications

(36 citation statements)

References 86 publications

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“…On the other hand, metagenomics analysis in this study showed that only chicken astroviruses were common to chickens that had been diagnosed with RSS via pathological lesions in the crypts of the small intestine. In addition, chicken astrovirus was not identified in the fecal virome of healthy chickens in Brazil [31]. Chicken astrovirus has been suggested as an etiological agent for RSS, but it could be detected in chickens without microscopic lesions.…”

Section: Discussionmentioning

confidence: 93%

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Kim

Kwon

Jang

et al. 2020

Virol J

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Background and aims: Runting-stunting syndrome (RSS) in chickens, also known as malabsorption syndrome, which is characterized by mild to severe enteritis and diagnosed through typical histopathologic examination as well as clinical signs, results in considerable economic losses. Despite the many studies carried out over decades to determine the etiologic agents of RSS involved in the disease, several outbreaks remained without the elucidation of, potentially multiple, etiologies involved. Methods: We performed comparative analysis of viral metagenomes from four chicken flocks affected with RSS using next-generation sequencing. Primers for the detection of chicken enteric viruses were designed from the sequencing data obtained with metagenomics. Multiplex reverse transcription-polymerase chain reaction (PCR) and PCR were performed to detect a variety of etiological agents previously described in natural cases of RSS.Results: The most abundant viral families identified in this study were Astroviridae, Picornaviridae, Parvoviridae, Caliciviridae, Reoviridae and Picobirnaviridae. Chicken astrovirus sequences were present in all four samples, suggesting an association between chicken astrovirus and RSS and chicken astrovirus as a candidate pathogen responsible for RSS. Picobirnavirus and the newly identified chapparvovirus were found in chickens in the Republic of Korea for the first time, and the genetic diversity of enteric viruses and viral communities was showed. Conclusions: Chicken astrovirus was consistently detected in broilers affected with RSS and the result of this study may contribute to knowledge of enteric diseases and viruses in chickens.

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Section: Discussionmentioning

confidence: 93%

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Kim

Kwon

Jang

et al. 2020

Virol J

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“…The presence of a parvoviral genome has also been reported in clinically healthy chicken and turkey flocks (2-17 weeks old). Occurrence of these viruses in the healthy avian gut and its association is unclear (Domanska-Blicharz et al, 2012Devaney et al, 2016;Lima et al, 2017). Histologically, the small intestine has acute catarrhal enteritis and moderate to severe distension of crypts with associated eosinophilic intranuclear inclusions within epithelial cells.…”

Section: Clinical Features Pathology and Pathogenesismentioning

confidence: 99%

“…Sequence-independent techniques, such as next generation sequencing, have allowed the detection of avian viral genome diversity from healthy and affected birds Kapgate et al, 2015). The comparative metagenomics studies revealed ChPV genomic sequence diversity identified from RSSaffected and unaffected broilers (Devaney et al, 2016;Lima et al, 2017), and divergent turkey parvovirus sequences from turkey faeces (Reuter et al, 2014). The characterization of the virome will provide a baseline for future studies comparing viral populations in healthy and diseased flocks.…”

Section: Diagnosismentioning

confidence: 99%

Avian parvovirus: classification, phylogeny, pathogenesis and diagnosis

et al. 2018

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Poultry parvoviruses identified during the early 1980s are found worldwide in intestines from young birds with enteric disease syndromes as well as healthy birds. The chicken parvovirus (ChPV) and turkey parvovirus (TuPV) belong to the Aveparvovirus genus within the subfamily Parvovirinae. Poultry parvoviruses are small, non-enveloped, single-stranded DNA viruses consisting of three open reading frames, the first two encoding the non-structural protein (NS) and nuclear phosphoprotein (NP) and the third encoding the viral capsid proteins 1 (VP1 and VP2). In contrast to other parvoviruses, the VP1-unique region does not contain the phospholipase A2 sequence motif. Recent experimental studies suggested the parvoviruses to be the candidate pathogens in cases of enteric disease syndrome. Current diagnostic methods for poultry parvovirus detection include PCR, real-time PCR, enzyme linked immunosorbent assay using recombinant VP2 or VP1 capsid proteins. Moreover, sequenceindependent amplification techniques combined with next-generation sequencing platforms have allowed rapid and simultaneous detection of the parvovirus from affected and healthy birds. There is no commercial vaccine; hence, the development of an effective vaccine to control the spread of infection should be of primary importance. This review presents the current knowledge on poultry parvoviruses with emphasis on taxonomy, phylogenetic relationship, genomic analysis, epidemiology, pathogenesis and diagnostic methods. ARTICLE HISTORY

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“…Catarina, in addition to previously reported isolates of FAdV-I and ChPV from the state of Rio Grande do Sul (LIMA et al, 2017). CAstV was found in samples from Minas Gerais, and the other member of the family, Astroviridae (ANV), was found in samples from Ceara, Paraiba, Pernambuco, Bahia, Minas Gerais, and São Paulo, increasing the previously reported distribution of astroviruses in the states of Rio Grande do Sul, Parana, and Mato Grosso (ESPINOZA et al, 2016a(ESPINOZA et al, , 2016b.…”

Section: Phylogenetic Analysissupporting

confidence: 55%

>b<Detection and molecular characterization of enteric viruses in commercial birds >/b<

Duque¹

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Ao meu querido filho Emilio José, que com seu espírito me motiva todos os dias para superar os desafios que a vida me reserva, procurando sempre o sucesso para compartilhá-lo com ele. A todos que eu amo, minha esposa Cristina, meus pais Wilson e Lidia, meus irmãos Consuelo e Wilmer, meus sobrinhos e sobrinhas Katrin, Brenda, Jéssica, Aaron, Jaidé, Michu e Samy, para quem compartilho meu esforço e aliás mostro que a perseverança pode fazer com que todos os objetivos sejam alcançados, independentemente dos obstáculos que temos em nossas vidas. AGRADECIMENTOS A Deus que sempre me guiou no melhor caminho em busca da felicidade. Aos professores Antonio y Claudette, pela amizade e apoio académico, emocional e familiar. Aos meus pais que sempre se esforçaram para me dar o melhor em todas as fases da minha vida, por seus sacrifícios, compreensão, paciência e amor, especialmente nos momentos mais difíceis da minha vida. A minha esposa Cristina, que esteve do meu lado incondicionalmente em todos os momentos da nossa aventura fora do país. À Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación (SENESCYT). Ao meu amigo e professor Luis que confiou em mim desde o início dos meus estudos e que abriu as portas de sua casa quando eu precisei. Aos meus colegas do laboratório Ruy, Mauricio, Alexander, Anderson, pela convivência.

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Faecal virome of healthy chickens reveals a large diversity of the eukaryote viral community, including novel circular ssDNA viruses

Cited by 52 publications

References 86 publications

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Avian parvovirus: classification, phylogeny, pathogenesis and diagnosis

>b<Detection and molecular characterization of enteric viruses in commercial birds >/b<

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