Faecal carriage of multidrug-resistant and extended-spectrum β-lactamase-producing Enterobacterales in people living with HIV in Yaoundé, Cameroon

Dimani, Brice Davy; Founou, Raspail Carrel; Zemtsa, Jessica Ravalona; Mbossi, Aurélia; Koudoum, Patrice Landry; Founou, Luria Leslie; Kouanfack, Charles; Sone, Lucien Honoré Etame

doi:10.1016/j.jgar.2023.07.021

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Extended-spectrum β-lactamase producing-E. coli (ESBL-Ec) has already been described as a public health issue that needs to be urgently addressed in humans (10,11,13) and animals in Cameroon (14,15). This study aims at determining the resistance genes and virulence factors of ESBL-Ec isolated from childhood diarrhoea during cholera outbreak in Yaoundé, Cameroon.…”

Section: Discussionmentioning

confidence: 99%

“…The genomic DNA of ESBL-Ec was extracted using a method previously described (13). Ampli cation of resistant genes including bla CTX−M, bla TEM, bla SHV, bla OXA−48, tet(A) and tet(B) were performed by conventional and multiplex methods using a thermal cycler BIO-RAD T100 (Bio-Rad Laboratories, Marnes-la-Coquette, France).…”

Section: Polymerase Chain Reaction Methods (Pcrs)mentioning

confidence: 99%

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Occurrence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli isolated from childhood diarrhoea in Yaoundé, Cameroon

Saleh,

ZANGUE,

Founou

et al. 2024

Preprint

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Introduction: Extended-spectrum β-lactamase (ESBL)-producing pathogenic E. coli is a global public health issue, especially in sub-Saharan African countries such as Cameroon. It contributes to increase hospital length of stay, morbidity, mortality, and economic costs because of treatment failures. This study aims at determining the resistance and virulence profiles of ESBL-E. coli among childhood diarrhoea during the cholera outbreak in Yaoundé, Cameroon. Materials and Methods: During a four-month period from March 1st to June 30th, 2023, stool samples were collected from 90 under five children presenting clinical signs of gastroenteritis and attending four hospitals in Yaoundé, Cameroon. Bacterial identification was done using API20E and antimicrobial susceptibility test was performed using the Kirby-Bauer disc diffusion method. After extraction, genomic DNA was subjected to conventional and multiplex polymerase chain reaction (PCR) for detection of resistance and virulence genes. Statistical analysis was performed using Epi infoTM (7.2.5.0). Statistical significance was considered at a p-value <0.05. Results: Out of 150 patients contacted, 90 patients were enrolled, 84 samples were collected, 52.38%(44/84) and 3.57%(03/84) were confirmed as extended-spectrum β-lactamase and carbapenemase-producing E. coli respectively. The risk factors were analyzed, and children who drank natural fruit juice (OR: 0.4, p-value: 0.03) were found to be significantly associated with ESBL-Ec. The ESBL-Ec isolates showed a high level of resistance to amoxicillin-clavulanic acid, cefotaxime, ceftazidime, cefepime, colistin, and tetracycline. The blaCTX-M was more prevalent ß-lactamase resistance gene. The tetracycline resistance genes tet(A) and tet(B) were also detected. The most important virulence genes detected were FimH (81.81%) and papA (79.54%). Conclusion: These findings suggest implementing routine surveillance and screening for antimicrobial resistance. Antimicrobial stewardship strategies (ASP) need to be implemented to curb the emergence and dissemination of ESBL-Ec. In addition, a national surveillance program for antimicrobial resistance needs to be implemented at local and regional levels in order to reduce morbidity in Cameroon.

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Section: Discussionmentioning

confidence: 99%

Section: Polymerase Chain Reaction Methods (Pcrs)mentioning

confidence: 99%

Occurrence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli isolated from childhood diarrhoea in Yaoundé, Cameroon

Saleh,

ZANGUE,

Founou

et al. 2024

Preprint

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“…Genomic DNA was extracted from all ESBL-E. coli and K. pneumoniae isolates by boiling method as previously described [11]. Detection of bla CTX−M, bla TEM and bla OXA−48 genes was carried out by multiplex-PCR method using a BIO-RAD thermal cycler (Bio-Rad Laboratories, Mames-la coquette, France).…”

Section: Conventional and Multiplex Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

High prevalence of multidrug resistant and extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in West region, Cameroon.

Bayaba,

Founou,

Tchouangueu

et al. 2024

Preprint

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Background Antimicrobial resistance remains a worldwide health problem with serious societal and economical repercussions. Multidrug resistant and Extended-Spectrum β-Lactamase producing-Enterobacterales (ESBL-E) are pathogens of critical public health priority that urgently require the research and development of new drugs. This study aims to determine the prevalence and assess the genes conferring resistance to β-lactams among Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infections (UTIs) in the West region, Cameroon.Methods A cross-sectional study was conducted among two healthcare facilities during a four-month period from February to May, 2023. All mid-stream urine samples were collected from UTIs patients. The Escherichia coli and K. pneumoniae strains were identified using Enterosystem 18R kit following the manufacturer instructions. The antimicrobial susceptibility test (AST) was performed using the Kirby-Bauer disk diffusion method. The screening of ESBL production was done using ESBL ChromAgar medium combined with the double-disk synergy test (DDT). Antimicrobial resistance genes were detected using polymerase chain methods. The data analysis was performed using Excel 2016 and IBM SPSS version 20.Results A total of 215 urine samples were collected and analyzed during the study period. A 31.62% (68/215) prevalence of Enterobacterales was detected with prevalence of 79.41% (54/68) and 14.70% (10/68) for Escherichia coli and Klebsiella pneumoniae respectively. The overall prevalence of ESBL-Enterobacterales was 64.70% (44/68). About 82% (36/44) of isolates were MDR and high antimicrobial resistance was observed for amoxicillin + clavulanic acid and ceftazidime. The resistance genes detected were blaCTX−M, blaTEM, tet(B) and tet(A), respectively.Conclusion The findings of this study highlight the high burden of MDR and ESBL-E. coli and K. pneumoniae isolates from UTIs. The study emphasizes the necessity of routine screening and monitoring of antimicrobial resistance in healthcare facilities and community settings. It is critical to implement antimicrobial stewardship programs in the country and infection prevention and control (IPC) measures in hospital settings.

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High prevalence and genetic diversity of multidrug-resistant and extended-spectrum ß-lactamase-producing Escherichia coli and Klebsiella pneumoniae in mothers and neonates in a Cameroonian labor ward

Njeuna,

Founou,

Founou

et al. 2024

American Journal of Infection Control

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Faecal carriage of multidrug-resistant and extended-spectrum β-lactamase-producing Enterobacterales in people living with HIV in Yaoundé, Cameroon

Cited by 5 publications

References 23 publications

Occurrence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli isolated from childhood diarrhoea in Yaoundé, Cameroon

Occurrence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli isolated from childhood diarrhoea in Yaoundé, Cameroon

High prevalence of multidrug resistant and extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in West region, Cameroon.

High prevalence and genetic diversity of multidrug-resistant and extended-spectrum ß-lactamase-producing Escherichia coli and Klebsiella pneumoniae in mothers and neonates in a Cameroonian labor ward

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