Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.

Phosphorylation of the N‐terminal domain of Xenopus TATA‐box binding protein by DNA‐dependent protein kinase depends on the C‐terminal core domain

“…Clearly, this domain is not required for basic promoter function and for the assembly with pol I-, pol II-and pol III-specific TBP-associated factors (TAFs) [29][30][31]. However, while some studies found that the N-terminal domain is also dispensable for activated transcription [32], others reported a requirement for the N-terminal domain for activated pol II transcription and for transcription from TATA-containing pol III promoters [33,34].…”

Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA‐binding protein and a novel transcriptional activator, TIP120

“…1B), as do other known TBP-binding proteins (Zhou et al 1993), suggesting that the C-terminal domain of TBP may be a common target for most TBP-interactable proteins. We do not know the reason why we could not identify known TBP-associated factors (TAFs) in our TIP preparations.…”

Multimerization of the mouse TATA-binding protein (TBP) driven by its C-terminal conserved domain

“…Plasmids and DNAs An NdeI site was created at translation initiation site of the mouse TBP cDNA (26) 10 ng/,u poly(dG-dC). Electrophoresis proceeded as previously reported (31) using a 5% polyacrylamide gel.…”

“…Hence, TBP includes two characteristics: DNA (TATA-box) binding and protein binding. In contrast to ordinary transcription regulators, TBP can interact with a number of proteins such as TBPassociated factors consisting of SLI, TFIID and TFIIIB complexes (4)(5)(6)(7)(8)(9)(10), general transcription factors (TFIIB, TFIIA and a subunit of RNA polymerase 11) (11)(12)(13)(14), as well as transcription regulatory factors (sequence-specific DNA-binding proteins) and other regulators (15)(16)(17)(18)(19)(20)(21)(22).…”