Factors related to the composition and diversity of wound microbiota investigated using culture-independent molecular methods: a scoping review

Kunimitsu, Mao; Kataoka, Yukie; Nakagami, Gojiro; Weller, Carolina; Sanada, Hiromi

doi:10.5582/ddt.2021.01036

Cited by 4 publications

(5 citation statements)

References 50 publications

(92 reference statements)

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“…The aetiological causes of the diversity reported in DFU are diverse and may have links with hygiene practices, cultural diversity, geographical variations, awareness, antibacterial treatment and so on. [ 69 , 81 , 82 ] Sampling methods are also reported to influence bacterial diversity. [ 8 , 83 , 84 ] Since the abundance of aerobic/anaerobic and Gram-positive/Gram-negative bacteria reside at different sites of DFU, the sampling methods also contribute to bacterial diversity patterns.…”

Section: Discussionmentioning

confidence: 99%

Diabetic Foot Ulcer in India: Aetiological Trends and Bacterial Diversity

Datkhile¹,

Kale²,

Karande³

2023

Indian Journal of Endocrinology and Metabolism

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Diabetes is one of the most prevalent epidemic metabolic disorders, responsible for a significant amount of physical, psychological and economic loss in human society. Diabetic foot ulcer (DFU) is one of the extreme pathophysiological consequences of diabetes. Bacterial infection is the most important cause of chronic DFU. Bacterial species or their biofilms show multidrug resistance, which complicates DFU and consequently leads to amputation of the infected part. Since the Indian population comprises diverse ethnic and cultural groups, this could influence the aetiology of diabetic foot infections and bacterial diversity. We reviewed 56 articles published from 2005 to 2022 on the microbiology of DFU and extracted the data on study location, number of patients analysed in the study, pathophysiological complications, age of the patients, sex of the patient, type of bacteria, type of infection (mono or polymicrobial), predominant bacteria (Gram-positive or Gram-negative), predominant isolates and multiple drug resistance (tested or not). We analysed data and described aetiological trends in diabetic foot infections and bacterial diversity. The study revealed that Gram-negative bacteria are predominant as compared to Gram-positive bacteria in individuals with diabetes with DFU in India. Escherichia coli , Pseudomonas aeruginosa , Klebsiella sp. and Proteus sp. were the most predominant Gram-negative bacteria, while Staphylococcus aureus and Enterococcus sp. were the major Gram-positive bacteria in DFU. We discuss bacterial infections in DFU in the context of bacterial diversity, sampling methods, demography and aetiology.

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Section: Discussionmentioning

confidence: 99%

Diabetic Foot Ulcer in India: Aetiological Trends and Bacterial Diversity

Datkhile¹,

Kale²,

Karande³

2023

Indian Journal of Endocrinology and Metabolism

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“…In this and other investigations ( 34 ) greater alpha-diversity indices were associated with negative biological effects. In contrast, others have associated lower values with disease and disease severity both in horses ( 15 , 43 ) and people ( 42 , 44 ) suggesting that the biological relevance of diversity indices are yet to be fully understood ( 6 ). Recent evidence suggests that rather than diversity, dynamic stability of microbial communities may be indicative of a host’s immune response and play a more relevant biological role ( 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past decade and facilitated by advances in DNA detection and identification, the importance of microbiota in health and disease has increasingly been recognized (1). This progress has also highlighted the role of bacterial communities in chronic wound healing of people (2)(3)(4)(5)(6). Consequently, the potential for topical interventions or products to modulate microbiota and improve outcomes has been explored (7)(8)(9)(10)(11) but determinants of microbial communities remain poorly understood (6).…”

Section: Introductionmentioning

confidence: 99%

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Wound healing of experimental equine skin wounds and concurrent microbiota in wound dressings following topical propylene glycol gel treatment

Labens,

Raidal,

Borgen-Nielsen

et al. 2023

Front. Vet. Sci.

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IntroductionTopical wound treatments rely on carrier formulations with little to no biological impact. The potential for a common vehicle, a propylene glycol (PG) gel, to affect wound healing measures including microbiota is not known. Microbiome characterization, based on next generation sequencing methods is typically performed on tissue or directly obtained wound fluid samples. The utility for primary wound dressings to characterize equine wound microbiota in the context of topical treatments is currently unknown. This investigation reports the topical effect of an 80% PG based gel on wound healing and microbiota in wound dressings.MethodsExperiments were performed in six mature horses utilizing a surgical, distal limb wound model, histology of sequential wound biopsies, photographic wound measurements and microbiota profiling via 16s rRNA sequencing of wound dressing samples. Experimental wounds were surveyed for 42 days and either treated (Day 7, 14, 21 and 28; at 0.03 ml/cm2) or unexposed to the PG gel. Wound surface area, relative and absolute microbial abundances, diversity indices and histologic parameters were analyzed in the context of the experimental group (treatment; control) using qualitative or quantitative methods depending on data characteristics.ResultsCompared to controls, treatment slowed the wound healing rate (17.17 ± 4.27 vs. 18.56 ± 6.3 mm2/day), delayed the temporal decline of polymorphonucleated cells in wound beds and operational taxonomic units (OTU) in wound dressings and lowered alpha-diversity indices for microbiota in primary wound dressing. Relative abundances of OTUs were in line with those previously reported for equine wounds. Clinical outcomes 42 days post wounding were considered similar irrespective of PG gel exposure.DiscussionResults highlight the potential for vehicle exposure to alter relevant wound outcome measures, imposing the need for stringent experimental control measures. Primary wound dressings may represent an alternate sample source for characterization of the wound microbiome alleviating the need for additional interventions. Further studies are warranted to contrast the microbiome in wound dressings against that present on wound surfaces to conclude on the validity of this approach.

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“…On the other hand, pathogenic microorganisms can cause a delayed wound healing response. 111,112 Education is an imperative in wound care, this paper provides the basis for enlightening the clinical readership as to the importance of the peri-wound skin in the processes of wound healing. By gaining this understanding it is hoped that this will translate into good practice and provide better clinical outcomes in terms of wound healing and peri-wound skin care.…”

Section: Dressing Choice To Limit Peri-wound Skin Damagementioning

confidence: 99%

The importance of periwound skin in wound healing: an overview of the evidence

Rippon

Rogers²,

Ousey

et al. 2022

J Wound Care

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The importance of the periwound skin as the ‘organ of healing’ in the skin should not be underestimated. This paper explores the numerous healing processes originating in the periwound skin, without which healing progression would not occur. This paper also explores how damage to the periwound skin is ubiquitous and can occur because of underlying pathology, misdiagnosis, mismanagement or the use of inappropriate treatments. As such, it is imperative that management strategies, including correct assessment of both wound bed and periwound skin, identify the correct treatment choice to lead to better patient/wound outcomes. Declaration of interest: The authors have no conflicts of interest.

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Factors related to the composition and diversity of wound microbiota investigated using culture-independent molecular methods: a scoping review

Cited by 4 publications

References 50 publications

Diabetic Foot Ulcer in India: Aetiological Trends and Bacterial Diversity

Diabetic Foot Ulcer in India: Aetiological Trends and Bacterial Diversity

Wound healing of experimental equine skin wounds and concurrent microbiota in wound dressings following topical propylene glycol gel treatment

The importance of periwound skin in wound healing: an overview of the evidence

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